Dmp1 (NM_016779) Mouse Untagged Clone

CAT#: MC216954

Dmp1 (untagged) - Mouse dentin matrix protein 1 (Dmp1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dmp1
• Synonyms: AG1; AV020965; Dmp; DMP-1; PP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216954 representing NM_016779
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGACTGTCATTCTCCTTGTGTTCCTTTGGGGGCTGTCCTGTGCTCTCCCAGTTGCCAGATACCACA
  ATACTGAATCTGAAAGCTCTGAAGAGAGGACGGGTGATTTGGCTGGGTCACCACCACCACCCACGAACAG
  TGAGTCATCAGAAGAAAGTCAAGCTAGCCCAGAGGGACAGGCAAATAGTGACCACACGGACAGCAGTGAA
  TCTGGAGAGGAGCTGGGCTACGACAGAGGCCAGTACAGACCGGCTGGTGGACTCTCTAAGAGTACGGGGA
  CCGGCGCCGATAAGGAGGATGATGAAGACGACAGTGGAGATGATACCTTTGGCGATGAGGACAATGATCT
  AGGGCCCGAAGAAGGACAGTGGGGAGGACCCTCCAAACTGGACAGTGATGAGGACTCCACAGACACCACA
  CAGTCCAGTGAAGACAGCACCTCTCAAGAAAACAGTGCCCAAGATACCCCCAGCGACAGCAAAGACCACG
  ACAGTGAGGATGAGGCAGACAGCCGGCCTGAGGCAGGCGACTCCACTCAGGACAGTGAGAGTGAGGAACA
  GCGGGTGGGAGGTGGCAGCGAGGGGGAGAGTAGCCACGGGGACGGTTCTGAGTTCGATGATGAAGGGATG
  CAGAGCGACGACCCCGAGAGTACCAGGAGCGATCGAGGCCACGCCAGAATGAGCAGCGCTGGTATCAGGT
  CGGAAGAATCTAAAGGGGACCACGAGCCCACGAGCACTCAGGATTCAGATGACAGCCAGTCTGTGGAATT
  TTCAAGCAGGAAGTCCTTCAGAAGGTCCCACGTCTCTGAGGAAGACTACAGAGGTGAGCTTACTGACAGC
  AACAGCAGGGAAACCCAGAGCGACTCCACGGAGGATACGGCCTCCAAGGAGGAAAGCAGGAGCGAGTCTC
  AGGAGGACACAGCCGAGAGCCAGTCCCAGGAAGATAGCCCAGAGGGGCAAGACCCCAGCAGTGAGTCCAG
  CGAAGAGGCTGGTGAGCCATCCCAGGAAAGCAGCAGCGAATCTCAGGAAGGGGTGACCAGCGAGTCCAGG
  GGTGACAACCCAGATAACACAAGTCAGGCAGGAGACCAAGAAGACAGTGAGTCCAGTGAGGAGGACAGCC
  TGAACACATTCTCCAGCTCAGAAAGCCAGTCCACCGAGGAGCAAGCTGACAGCGAGTCCAACGAGAGCCT
  CAGCCTCTCCGAGGAGAGTCAGGAGTCGGCCCAGGATGGTGACAGCTCCAGCCAGGAAGGCCTGCAGTCC
  CAGAGCGCATCCACTGAGAGCAGGAGCCAGGAGAGCCAGTCTGAGCAGGACAGCCGTTCTGAGGAAGACA
  GTGACTCTCAGGACAGTAGCCGATCCAAAGAAGAGAGCAACTCCACAGGGAGCGCTTCCAGCAGCGAGGA
  GGACATCCGTCCCAAGAACATGGAAGCTGACAGTAGGAAACTAATAGTTGATGCTTACCACAACAAACCC
  ATCGGGGACCAAGATGACAATGACTGTCAGGACGGCTACTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_016779
• Insert Size: 1512 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016779.2, NP_058059.2
• RefSeq Size: 2760 bp
• RefSeq ORF: 1512 bp
• Locus ID: 13406
• UniProt ID: O55188
• Cytogenetics: 5 50.61 cM
• Gene Summary: May have a dual function during osteoblast differentiation. In the nucleus of undifferentiated osteoblasts, unphosphorylated form acts as a transcriptional component for activation of osteoblast-specific genes like osteocalcin. During the osteoblast to osteocyte transition phase it is phosphorylated and exported into the extracellular matrix, where it regulates nucleation of hydroxyapatite (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same protein. Sequence Note: This sequence has been modified as follows: removed 17/38 bp suspected to be vector contamination from the 5'/3' end.