Kcns3 (NM_173417) Mouse Untagged Clone

CAT#: MC216898

Kcns3 (untagged) - Mouse potassium voltage-gated channel, delayed-rectifier, subfamily S, member 3 (Kcns3), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kcns3
• Synonyms: D12Ertd137e
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216898 representing NM_173417
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGTTTGGTGAGTTTTTCCATCGCCCTGGACAAGATGAGGAACTTGTCAACTTGAACGTGGGGGGCT
  TTAAGCAGTCTGTGGATCAAAGTACACTCCTGCGGTTCCCTCACACACGACTGGGAAAGCTGCTTACCTG
  CCACTCTGAGGAGGCCATTCTGGAGCTGTGTGATGACTACAGCGTGGCAGATAAAGAGTACTACTTTGAT
  CGGAACCCCTTCCTGTTCAGATACGTCTTGAACTTTTATTACACAGGGAAGCTGCATGTGATGGAGGAAC
  TGTGTGTCTTCTCCTTCTGCCAGGAGATCGAGTACTGGGGCATCAATGAGCTCTTCATTGACTCCTGCTG
  TAGCAGTCGGTACCAGGAGCGCAAGGAGGAGAGCCACGACAAGGACTGGGACCAGAAAAGCAACGATGTG
  AGCACAGACTCCTCCTTTGAAGAATCGTCTCTGTTTGAGAAAGAGCTGGAGAAGTTTGATGAGCTGAGAT
  TTGGTCAGCTCCGAAAGAAGATCTGGATTCGAATGGAAAATCCAGCTTACTGCCTGTCGGCCAAGCTCAT
  TGCCATCTCCTCCTTGAGCGTGGTGCTGGCTTCCATAGTGGCCATGTGTGTGCACAGCATGTCGGAATTC
  CAGAACGAGGATGGAGAAGTGGATGACCCTGTGCTGGAAGGTGTGGAGATTGCCTGCATTGCATGGTTTA
  CTGGTGAGCTAGCCATCAGGCTGGTTGCTGCTCCATCACAAAAGAAGTTCTGGAAAAACCCTCTGAACAT
  CATTGACTTTGTTTCTATCATTCCCTTCTATGCCACGTTGGCTGTGGACACCAAGGAAGAAGAGAGTGAG
  GACATTGAGAATATGGGCAAGGTGGTCCAGATCCTTCGGCTCATGAGGATTTTCCGAATTCTGAAGCTTG
  CCCGGCACTCTGTAGGGCTTCGGTCTCTTGGGGCCACACTGAGGCACAGTTACCATGAGGTGGGGCTACT
  GCTTCTCTTCCTTTCTGTGGGCATCTCCATCTTCTCTGTGCTTATCTACTCTGTGGAGAAAGATGAACAC
  AAGTCCAGTCTCACCAGCATCCCCATCTGCTGGTGGTGGGCCACTATCAGTATGACCACAGTGGGCTATG
  GAGACACCCACCCAGTCACCTTAGCTGGGAAAATCATTGCAAGCACATGTATTATCTGTGGAATCTTAGT
  GGTAGCCCTCCCCATTACCATCATCTTCAACAAGTTTTCCAAGTACTACCAGAAGCAGAAAGACATGGAA
  GTGGACCAGTGCAGCGAGGACCCACCAGAGAAGTGCCATGAGCTACCGTACTTTAACATTAGGGACGTTT
  ATGCACAGCAAGTACATGCCTTCATCACCAGTCTGTCTTCCATTGGCATCGTGGTCAGTGATCCTGACTC
  CACAGATGCTTCGAGCGTTGAAGACAATGAGGATGCTTACAACACTGCATCCCTGGAGAACTGTACTGGA
  AAATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_173417
• Insert Size: 1476 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173417.3, NP_775593.1
• RefSeq Size: 2933 bp
• RefSeq ORF: 1476 bp
• Locus ID: 238076
• UniProt ID: Q8BQZ8
• Cytogenetics: 12 5.58 cM
• Gene Summary: Potassium channel subunit that does not form functional channels by itself. Can form functional heterotetrameric channels with KCNB1; modulates the delayed rectifier voltage-gated potassium channel activation and deactivation rates of KCNB1. Heterotetrameric channel activity formed with KCNB1 show increased current amplitude with the threshold for action potential activation shifted towards more negative values in hypoxic-treated pulmonary artery smooth muscle cells.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.