Atg14 (NM_172599) Mouse Untagged Clone

CAT#: MC216889

Atg14 (untagged) - Mouse VATG14 autophagy related 14 homolog (S. cerevisiae) (Atg14), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Atg14
• Synonyms: 4832427M01; Atg14L; D14Ertd114e; D14Ertd436e
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216889 representing NM_172599
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCTCCCAGTGGGAAGGGATCTTGGACGCCCGAGGCTCCTGGTTTTGGGCCGCGGGCGCTAGCAC
  GGGACCTGGTGGACTCGGTGGACGACGCCGAGGGCCTTTACGTGGCTGTTGAGCGGTGTCCTCTGTGCAA
  CACCACTCGCCGGCGGTTGACTTGCGCCAAGTGCGTCCAGAGCGGTGATTTCGTCTATTTCGACGGCCGC
  GACCGGGAGAGGTTTATTGACAAGAAGGAAAGACTAAGCCAACTTAAGAACAAGCAAGAAGAATTTCAGA
  AAGAAGTACTAAAAGCTATGGAAGGAAAGCGGCTTACAGATCAGTTGAGATGGAAAATAATGTCATGCAA
  GATGAGGATTGAACAGCTGAAGCAAACAATATGTAAAGGAAATGAGGAAATGAAGAAAAATTCTGAAGGT
  CTCCTCAAGAACAAGGAAAAGAACCAGAAGCTTTACAGCCGAGCACAGCGGCACCAAGAGAAAAAGGAGA
  AGATTCAGCGGCACAACCGCAAGCTTGGGGACCTGGTGGAGAAGAAGACCATTGACTTGAAGAGTCACTA
  TGAGCGGTTGGCGCGGCTTCGAAGGTCACACATCCTAGAGCTCACCTCCATCATATTCCCAATCGACGAA
  GTGAAGACTTCTGGGAGAGACCCTGCAGACGTGTCTTCAGAGACTGACAGTGCCATGACCTCAAGCATGG
  TGAGCAAGCTTGCTGAGGCCCGGAGGACAACTTACCTCTCTGGAAGATGGGTCTGTGATGACCACAATGG
  TGACACCAGCATTAGCATCACAGGCCCGTGGATTAGCCTACCAAACAACGGGGACTACTCTGCTTACTAC
  AATTGGGTAGAAGAGAAGAAAACAACCCAAGGACCTGACATGGAGCATAACAACCCCGCCTACACTATCA
  GCGCCGCGCTGGGCTACGCCACGCAGCTCGTCAACATTGTGTCTCACATACTTGACATCAATCTTCCCAA
  AAAGCTGTGCAACAGCGAGTTCTGTGGCGAAAACCTCAGCAAGCAGAAACTGACACGCGCAGTGAGGAAA
  CTGAACGCAAACATCCTTTACCTTTGTTCTTCTCAGCATGTAAATCTGGATCAGTTGCAACCACTGCACA
  CACTCAGGAACCTGATGCACTTGGTCAGCCCGCGCTCTGAGCACCTAGGCAGGTCAGGACCCTTTGAAGT
  TCGAGCAGACCTCGAGGAGTCCATGGAATTTGTGGACCCTGGAGTTGCTGGAGAATCAGACGCGAGTGGA
  GATGAGCGTGTAAGCGATGAGGAGACTGACCTGGGCACAGACTGGGAGAACCTGCCAAGCCCCCGATTCT
  GTGACATCCCTTCCCAGCCGGTGGAAGTGTCCCAGAGCCAGAGCACCCAGGTGTCCCCACCCATTGCCAG
  CAGCAGCGCTGGTGGGATGATCTCCTCCGCTGCGGCCTCGGTGACCTCTTGGTTCAAAGCTTACACTGGA
  CACCGCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_172599
• Insert Size: 1479 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172599.4, NP_766187.1
• RefSeq Size: 3466 bp
• RefSeq ORF: 1479 bp
• Locus ID: 100504663
• UniProt ID: Q8CDJ3
• Cytogenetics: 14 24.6 cM
• Gene Summary: Required for both basal and inducible autophagy (PubMed:19270696, PubMed:19270693). Determines the localization of the autophagy-specific PI3-kinase complex PI3KC3-C1 (By similarity). Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine (PubMed:19270696, PubMed:19270693). Promotes BECN1 translocation from the trans-Golgi network to autophagosomes (By similarity). Enhances PIK3C3 activity in a BECN1-dependent manner. Essential for the autophagy-dependent phosphorylation of BECN1 (By similarity). Stimulates the phosphorylation of BECN1, but suppresses the phosphorylation of PIK3C3 by AMPK (PubMed:23332761). Binds to STX17-SNAP29 binary t-SNARE complex on autophagosomes and primes it for VAMP8 interaction to promote autophagosome-endolysosome fusion (By similarity). Modulates the hepatic lipid metabolism (PubMed:22992773).[UniProtKB/Swiss-Prot Function]