Trim6 (NM_001013616) Mouse Untagged Clone

CAT#: MC216873

Trim6 (untagged) - Mouse tripartite motif-containing 6 (Trim6), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Trim6
• Synonyms: C430046K18Rik; D7Ertd684e
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216873 representing NM_001013616
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTTCAACAGTCTTGGTGGACATCCGAGATGAAGTAACCTGCCCTATCTGCTTGGAGCTCCTGACAG
  AACCCCTGAGCATTGATTGTGGCCATAGCTTCTGCCAGGTCTGCATCATAGGAAACAGTAATAATTCAGT
  GTTCGGCCAAGGAGGGAGGAGCAGCTGTCCTGTGTGCCGGACCTCCTATCAGCCTGGGAACCTCCGTCCT
  AATCGGCACCTGGCCGCCATAGTGAAGAGGCTCAGAGAGGTTGCGTTGTGCCCTGGAAAACAACTCGAGG
  TCATTTTTTGTGCGCTTCATGGAGAGAAACTCCAGCTCTTTTGCAAGGAGGATGGGAAGTTAATTTGCTG
  GCTTTGTGAACGATCTCAGGAGCACCGTGGTCATCACACATTCCTCATGGAGGAGGTGGCCCAGGAGTAC
  CAGGACATGTTCCAGGAGTCTCTGAAGAAATTGAGGAGGGAGCAGCAGGAAGCCGAGAAGCTAAAAGCTC
  TTATCCAGGAGAAGAGGGAATCCTGGAAGAGTCAGGTGGAGCCTGAGAAACGCCGGATCCAGACAGAGTT
  TAAGCAGCTCCGAAGCATCCTGGACAGGGAGGAGCAGCGGGAACTGAAGAAACTGGAAGTGGAAGAGAGG
  AAGGGGCTGAGCATCATAGAAAAGGCCGAGGGTGACCTGATCCACCAGAGCCAGTCACTGAAGGACCTCA
  TCTCAGACCTGGAGCACCGGTGCCAGGGGTCCACCGTAGAACTGCTGCAGGATGTGGGTGATGTTACAAA
  AAGGAGTGAGTTCTGGACCCTGAGGAAGCCCCAAGCTCTCCCCACCAAGCTGAAAAGTTTGTTTCGAGCA
  CCGGATCTGAGGAAGATGCTAAAAGTCTTTAGAGAGCTGACAGATGTCCAAAGCTATTGGGTGGACGTGA
  CACTGAATCCACAGACGGCTAATTTAAATCTTGTCCTGTCGAAAAATCGGAGACAGGTGAGGTTTGTGGG
  TGCCCAGCTGTCCGAGCCATCCAGTCTGGAAGAACATTATGACTGTAGTGTCCTGGGCTCTCAGCACTTC
  TCCTCAGGAAAATACTACTGGGAAGTGGACGTGAGCAAGAAGACGGCATGGATCTTGGGTGTATGCAGTA
  CCCCGGTGGATCCCATGTTCTCTTTCAGCCAGTACTCCAGCAAGCAGGGCGCCTACTCCAGGTATCAGCC
  ACAGTGTGGATACTGGGTGATTGGCTTGCAGTGTAAGCACGAGTACAGAGCCTATGAGGATTCCTCCCCG
  TCCCTGCTCCTCTCCATGACCGTGCCACCTCGACGCATAGGGATTTTCTTAGACTGTGAGGCTGGCACGG
  TCTCCTTTTATAATGTCACAAACCACGGCTTGCCCATCTACACCTTCTCGAAGTATTACTTTCCTTCTGC
  CCTTTGTCCCTATTTTAATCCCTGCAGCTGTATAGTCCCGATGACCCTGCGGCGGCCAACTTCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001013616
• Insert Size: 1467 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001013616.2, NP_001013637.1
• RefSeq Size: 3827 bp
• RefSeq ORF: 1467 bp
• Locus ID: 94088
• UniProt ID: Q8BGE7
• Cytogenetics: 7 55.55 cM
• Gene Summary: E3 ubiquitin-protein ligase which ubiquitinates MYC and inhibits its transcription activation activity, maintaining the pluripotency of embryonic stem cells (PubMed:22328504). Involved in the synthesis of unanchored K48-linked polyubiquitin chains which interact with and activate the serine/threonine kinase IKBKE, leading to phosphorylation of STAT1 and stimulation of an antiviral response (PubMed:24882218).[UniProtKB/Swiss-Prot Function]