Btbd1 (NM_146193) Mouse Untagged Clone

CAT#: MC216872

Btbd1 (untagged) - Mouse BTB (POZ) domain containing 1 (Btbd1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Btbd1
• Synonyms: 1190005H08Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216872 representing NM_146193
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC
  GCCC
  
  ATGGCCTCGCTCGGGTCAGCCGCGGCCGGGGAGCCGGCGACCGGAGCTGAGGCGGAGCCCGGCCCTCCGG
  CCCCGCCGCCGCCTCCGCCGCCGCCACCGGCTCCCTCGCCGTCCGCTCTCGGGCCGCTGCTGCCCCTGCA
  GCGCGAGCCGCTGTACAACTGGCAGGCCACCAAGGCGTCGCTCAAGGAGCGCTTCGCCTTCCTCTTCAAC
  TCGGAGCTGCTGAGCGATGTGCGTTTCGTGCTGGGCAAGGGCCGAGGCGCGGCGGCCGCCGGGGGCCCGC
  AGCGCATCCCCGCCCACCGCTTCGTGCTGGCGGCCGGCAGCGCGGTCTTCGACGCCATGTTCAACGGCGG
  CATGGCCACGACGTCGGCCGAGATCGAGCTGCCGGACGTGGAGCCCGCCGCCTTCCTGGCGCTGCTGAGA
  TTTTTGTATTCAGACGAAGTTCAAATTGGTCCAGAAACCGTTATGACCACTCTTTATACTGCTAAGAAAT
  ATGCAGTCCCTGCCTTGGAAGCACATTGCGTAGAATTTCTCACCAAACATCTTAGGGCAGATAATGCCTT
  TATGTTGCTTACTCAGGCGCGGTTGTTCGATGAGCCCCAGCTGGCTAGTCTTTGTCTGGACACCATAGAC
  AAGAGCACAGTGGATGCAATCAGTGCGGAGGGGTTTACTGATATTGACATAGATACACTCTGTGCAGTTC
  TAGAAAGAGACACACTGAGTATCCGAGAAAGTCGACTTTTTGGAGCTATTGTACGTTGGGCAGAAGCAGA
  GTGTCAGAGACAACAACTGGCTGTGACATTTGGAAACAAACAGAAAGTTCTAGGAAAAGCACTTTCCTTA
  ATCCGCTTCCCACTGATGACAATTGAGGAGTTTGCAGCAGGTCCTGCTCAATCTGGAATTTTGTCAGACC
  GTGAAGTGGTAAACCTCTTTCTTCATTTTACCGTCAATCCTAAACCCCGAGTAGAGTACATTGATCGGCC
  ACGATGCTGCCTCAGAGGAAAGGAGTGCTGCATCAACCGATTCCAGCAAGTAGAGAGCCGCTGGGGTTAC
  AGTGGGACAAGCGATCGCATCAGGTTCACAGTTAACAGAAGAATCTCTGTAGTTGGATTTGGTTTGTATG
  GATCTATTCACGGGCCCACGGATTATCAAGTGAATATACAGATCATTGAATATGAGAAAAAACAAACCTT
  GGGACAGAATGATACTGGATTTAGTTGTGATGGGACTGCTAACACATTCAGGGTCATGTTCAAAGAACCT
  ATAGAGATCCTGCCCAATGTCTGTTACACAGCGTGCGCAACACTCAAGGGCCCAGATTCTCACTATGGCA
  CAAAAGGACTGAAGAAAGTAGTGCATGAGACCCCTGCTGCAAGCAAGACTGTTTTCCTATTTTTCAGTTC
  CCCTGGCAATAACAATGGCACATCAATAGAAGATGGACAAATACCAGAAATAATATTTTATACATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: AscI-MluI
• ACCN: NM_146193
• Insert Size: 1467 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_146193.2, NP_666305.2
• RefSeq Size: 3020 bp
• RefSeq ORF: 1467 bp
• Locus ID: 83962
• UniProt ID: P58544
• Cytogenetics: 7 D3
• Gene Summary: Probable substrate-specific adapter of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (By similarity). Seems to regulate expression levels and/or subnuclear distribution of TOP1, via an unknown mechanism (PubMed:15486563, PubMed:17462629). May play a role in mesenchymal differentiation where it promotes myogenic differentiation and suppresses adipogenesis (PubMed:15486563, PubMed:17462629).[UniProtKB/Swiss-Prot Function]