Chrne (NM_009603) Mouse Untagged Clone

CAT#: MC216784

Chrne (untagged) - Mouse cholinergic receptor, nicotinic, epsilon polypeptide (Chrne), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Chrne
• Synonyms: Ac; ACh; AChrepsilon; Acre; nAChRE
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216784 representing NM_009603
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGGGGCTCTGCTTGGTGCCCTGCTTCTCCTGACACTCTTTGGCAGAAGCCAGGGAAAGAATGAAG
  AGCTTAGCCTGTATCACCATCTCTTCGACAATTATGATCCAGAATGCCGGCCAGTTAGGAGACCTGAGGA
  CACTGTCACCATCACCCTCAAGGTCACCCTAACCAACCTCATCTCACTGAACGAGAAAGAAGAAACTCTG
  ACCACCAGTGTCTGGATTGGCATTGACTGGCACGACTATCGGCTCAACTACAGCAAGGACGATTTTGCAG
  GTGTAGGAATCCTCCGGGTCCCTTCAGAACATGTATGGCTGCCAGAGATTGTTCTAGAAAACAATATTGA
  TGGGCAGTTTGGAGTGGCCTACGACAGCAATGTTCTAGTCTATGAGGGAGGCTATGTGAGCTGGTTGCCC
  CCAGCCATCTACCGCAGCACCTGCGCAGTGGAGGTCACCTATTTCCCCTTTGACTGGCAGAACTGCTCTC
  TCATTTTTCGCTCCCAGACCTACAATGCTGAGGAGGTGGAGTTCATCTTTGCCGTGGATGACGACGGCAA
  TACCATCAACAAGATTGACATTGACACGGCAGCTTTTACCGAGAATGGAGAATGGGCCATAGACTACTGC
  CCAGGCATGATTCGCCGCTATGAGGGAGGTTCCACAGAAGGTCCTGGAGAAACTGACGTCATCTATACGC
  TCATCATCCGCCGGAAGCCGCTTTTTTACGTCATTAACATCATTGTGCCTTGCGTGCTCATTTCTGGCTT
  GGTGCTGCTCGCTTACTTCCTGCCTGCGCAGGCTGGTGGCCAGAAATGCACGGTCTCTATCAACGTCCTG
  CTAGCCCAGACTGTCTTCTTGTTTCTAATTGCCCAGAAAATTCCAGAGACTTCTCTGAGCGTGCCACTGC
  TGGGCAGGTATCTTATATTCGTCATGGTGGTTGCCACGCTCATTGTCATGAATTGCGTCATCGTGCTCAA
  CGTATCTTTGAGGACGCCAACGACTCATGCTACATCCCCTCGGCTGCGCCAGATTTTATTAGAGCTGCTG
  CCGCGTCTCCTGGGCTCGAGCCCACCCCCAGAGGATCCCCGAACTGCCTCACCAGCGAGGCGTGCCTCGT
  CTGTGGGCATTCTGCTCAGAGCGGAGGAGCTCATCTTGAAAAAGCCGCGGAGCGAACTCGTGTTTGAGGG
  TCAGAGGCATCGGCACGGAACTTGGACCGCAGCCCTCTGCCAGAACCTGGGTGCTGCAGCCCCAGAAATC
  CGCTGCTGTGTGGATGCTGTGAACTTTGTGGCTGAGAGCACAAGAGACCAGGAAGCCACTGGAGAGGAAC
  TGTCCGACTGGGTGCGTATGGGGAAGGCCCTGGACAATGTCTGTTTTTGGGCAGCTTTGGTGCTCTTCAG
  CGTTGGTTCTACTCTCATCTTCCTTGGGGGTTACTTCAACCAAGTTCCTGATCTCCCTTACCCACCGTGC
  ATCCAACCATGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009603
• Insert Size: 1482 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009603.1, NP_033733.1
• RefSeq Size: 1599 bp
• RefSeq ORF: 1482 bp
• Locus ID: 11448
• UniProt ID: P20782
• Cytogenetics: 11 43.14 cM
• Gene Summary: This gene encodes the epsilon subunit of the muscle-derived nicotinic acetylcholine receptor, a pentameric neurotransmitter receptor and member of the ligand-gated ion channel superfamily. The acetylcholine receptor changes subunit composition shortly after birth when the epsilon subunit replaces the gamma subunit seen in embryonic receptors. In mice, deficiency of this gene can lead to a decline in the number of nicotinic acetylcholine receptors at neuromuscular junctions and causes progressive muscle weakness, atrophy and premature death. Mutations in this gene serve as a pathophysiological model for human congenital myasthenia. Several alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Nov 2012]