Rufy4 (NM_001034060) Mouse Untagged Clone

CAT#: MC216757

Rufy4 (untagged) - Mouse RUN and FYVE domain containing 4 (Rufy4), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Rufy4
• Synonyms: F930048N03Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216757 representing NM_001034060
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCTTCATATGCTCACAGGACAAGCTGAAGACCTCACTAGGAAAAGGTCGTGCCTTTATCCGACTCT
  GCCTTGCCCGAGGCCAGCTGGCTGAGTCGATGCAGCTGTGCCTCCTGAACCCACAACTCACCAGGGAATG
  GTATGGCCCTCGGAGCCCTCTGTTGTGCGCTGAGCTCCAGGAAGACATCTTGGACTCTCTCTATGCCCTC
  AATGGAGTTGCCTTCAACTTGGACCTGCAGCGGCCAGACCTGGATGAAGCCTGGCCCATGTTCTCAGAGT
  CCCGCTGTTCCAGTCCCAGCCGGACCGGGAAAAGAAGACCCGGGAAACCCAAAGGTTTTCCAGAGGAGGT
  GAGATGCTCCAGGGGAGAGCAGCTGCAGGAACCAGACACTGGAGGAACCAGCTGTCTGCAGGATGCAACC
  AGAGAAGACCGCACACCTGACCTCTGTAAACCCTTGCAGCCAAGCCATCTTCCCACCTTTCTGGAAGAGA
  AGAGGGAGGATTCCAGAAGCCTCAGTTGTCCCCAGAGCACTTGGGAAACAGAAAGGGAAGGATTTCAGCT
  AGACCAGAAGGATGGAGGCCCAAAGCCCAGGAAATTCCTGGAAAACTCAACAGCCAGCATCCAGCAACAG
  AGGAGCAGGGCTAAGGATGTTAAAATGCAGCTGACAGGAAGGAAAGTTGAAGGAAAAGGAAGTCTGTCGG
  GGACAGAAGATCAGAGAACAACAGAAGGGATCCAGAAAAGGGCAGCAGATTGGGACCTTGGCCAGGGGCT
  GATGGCCCCCGGTCTTCAGGGAAGAGAAGATGCAGAGTTAGGATACAGGTGTGAGTGGAATCAGCCTGAT
  GTTCTCAGGCAATCCTGGGTCCTGGGAACAAAGAAAAGTTCTCCCACAGAGAAGCCACAAGAGTGGACAG
  GAGTGACCAGTGGAACCATGCAGGAGGATGGGTCAGAAGTGCCCTTGCAGCAGGAGGTGATCAAGGACCC
  TGGGTATGGGCTCCAGCTGGCAAAGGAACAGGCCCAGTGCCAGGAGCAGCTGCGGGCACAGGAAGCAGAA
  CTTCAGGCGCTCCAGGAGCAGCTCAGCAGGTGTCAAAAAGAAAGAGCCCTGTTGCAAGTGAAGCTGGAGC
  AGAAGCAGCAGGAGGCTGAGAGGAGGGACGCCATGTATCAGACAGAGCTTGAAGGGCAGAGGGACCTGGT
  CCAGGCCATGAAAAGGAGGGTGTTGGAACTGATCCATGAGAAGGACCTTCAGTGGCAGAGACTCCAGCAG
  CTGTCTACTGTGGCCCCTGGACACTGCATAGGCTGCAACAAGGTCTTCAGGCGTCTGTCTCGGCGGTATC
  CTTGCAGGCTCTGTGGAGGCCTGGTTTGCCACGCCTGCTCTGTAGACTACAAGAAGAGAGAGCGTTGCTG
  CCCAACCTGTGCCCAGCAGGAAGAAATCCAGGACACCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001034060
• Insert Size: 1440 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001034060.3, NP_001029232.2
• RefSeq Size: 2306 bp
• RefSeq ORF: 1440 bp
• Locus ID: 435626
• UniProt ID: Q3TYX8
• Cytogenetics: 1 C3
• Gene Summary: Positively regulates macroautophagy in primary dendritic cells. Increases autophagic flux, probably by stimulating both autophagosome formation and facilitating tethering with lysosomes. Binds to phosphatidylinositol 3-phosphate (PtdIns3P) through its FYVE-type zinc finger.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate splice site and and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.