Bpi (NM_177850) Mouse Untagged Clone

CAT#: MC216741

Bpi (untagged) - Mouse bactericidal permeablility increasing protein (Bpi), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Bpi
• Synonyms: 9230105K17Rik; Bpifd1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216741 representing NM_177850
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCTGGGCCCCTGACAACGTGCGGAAATGGTCAGCCCTGTTGCTGCTGGCCATCATTGGCACAGCCC
  TGACAGCCGCCACCGACCCTGGCTTCGTGGCCATGATCTCCCAGAAGGGTCTGGACTTCGCGTGCCAGCA
  GGGAGTGGTTGAGTTGCAGAAGGAGCTACAGGCTATCAGTGTTCCTGACTTCTCTGGGGTCTTTAAGATC
  AAGCACCTGGGAAAGGGTTCCTACGAATTCTACAGCATGGCTGTGGATGGATTTCATATTCCTAATCCCA
  AGATCGAGATGCTGCCCAGTGATGGCCTCCGAGTGTTCATCAAAGATGCCAGCATCAAGATCAATGGAAA
  GTGGATGTCAAGGAAGAACTTCCTCAAGGCCGGTGGCAATTTTGAACTGAGCATCCAGGGCGTCTCCATC
  TCCACCGACCTGATTCTGGGTAGTGACTCCTCAGGCCACATCACCACCATCTGCTCCAACTGCGACAGTC
  ACATTGACAGTGTCCATATCAAAATCTCAGGCAGCATGCTGGGGTGGCTGATCCGACTGTTCCACAGGAA
  AATTGAGACTTCCCTGAAAAACATCATTTACAAAAAGATCTGCAAGATCGTGAGAGACTCTGTGTCCTCC
  AAACTGCAGCCTTATCTGAAGACCCTTTCTGTGATAACCAGAGTGGATGATGTGACCTCGGTTGACTACA
  GTCTGCTGGCTCCCCTGACAACCACGAACCAGTTCCTGGAGGGGCAGCTAAAGGGGGAGTTTTTCTGGAG
  GGGACACCGAGACCCACTTCCCATTCACCCGCCAGTAATGAGGTTTGTGCCCAACGGTGCATACATGGTA
  TGCATGGGCATCTCTGACTACTTCTTCAACACTGAGGTATTGGCTTACCAACAGTCTGGAACCTTGAAGA
  TGACCCTGGGGGGCCAACTGTTATCAAATAATGGCAGATTCCAGCTGAACACTGACTTCCTTAGGACCTT
  CCTGCCCAAGGTGGCCAAGATGTTCCCCAGCATGGGGGTGCAGCTGCTCATCTCAGCGCCCGTGCCTGTG
  CACCTGAGCATACAGCCCTCAGGCCTCTCATTCAATCCTAAGCTGGAGACCCAGGCCTTTGTGGTCCTCC
  CCAACGCCTCCCTGGTCCCTCTCTTTGTTCTTGGCATGGTAAGAAGGAAAACAAATGCCTCTTTGGAAGT
  GGATGCTGAGGAGAACAGGCTCGTGGGAGAGATGAAGCTTGGCAGCAGGTGGTTGCTGGAATTGAAGGAA
  TCCAAATTTGGCCCCTTTAAGGTGGAGTACCTGGAGGATGTCATAAACTACCTCGTATCCACACTAGTGC
  TGCCCAAGATCAATGAGAGGCTGCGTAGAGGCTTCCCTCTCCCTTTGCCTGCTGGGATCCGGTTCAGCCA
  CTTCACGTTTTACCCCTACCAGAATTTCCTGTTGCTTGAAGCGGATTTACACCTTATCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_177850
• Insert Size: 1461 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177850.3, NP_808518.1
• RefSeq Size: 1779 bp
• RefSeq ORF: 1461 bp
• Locus ID: 329547
• UniProt ID: Q67E05
• Cytogenetics: 2 H1
• Gene Summary: The cytotoxic action of BPI is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).