Zc3hc1 (NM_172735) Mouse Untagged Clone

CAT#: MC216723

Zc3hc1 (untagged) - Mouse zinc finger, C3HC type 1 (Zc3hc1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Zc3hc1
• Synonyms: 1110054L24Rik; AU018540; AU019789; HSPC216; Nipa
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216723 representing NM_172735
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCTACCAGTGAGGGGCCGCTGTTTGCTGCGAGTATTGAAAAGACTTGGGGTTCAGTCGTTCGTT
  CCCCTGAAGGGACACCCCAGAAAGTTCGGGAGCTGATAGATGAAGGAATTGTCCCGGAAGAGGGAGGCAC
  AGAACCAAAGGACACAGCTGCCACATTCCAGTCAGTTGATGGATCGCCTCAAGCAGAACAGTCTCCATTG
  GAATCCACAAGCAAAGAAGCCTTCTTTCACAGAGTGGAAACCTTTTCTTCTTTGAAGTGGGCAGGGAAGC
  CCCCTGAGCTGTCTCCACTTATCTGCGCTAAATATGGTTGGGTCACAGTTGAATGTGACATGCTCAAGTG
  CTCCAGCTGCCAGGCTTTCCTCTGTGCCAGCCTGCAGCCCACGTTTGACTTTGGCAGATATAAGGAGCGA
  TGTGCTGAGCTCAAGAAATCCTTGTGCAGTGCCCATGAGAAGTTCTGTTTTTGGCCAGACAGCCCCTCTC
  CAGATAGATTTGGGATGTTGCCATTGGGTGAGCCTGCTGTTCTCATCAGTGAGTTCCTGGACCGGTTTCA
  AAGCCTTTGCCATTTGGACCTCCAACTTCCTTCTCTGAGGCCAGAGGACTTGAAAACTATGTGCTTGACA
  GAAGACGCGGTCAGTGCCCTGCTGCACCTGCTGGAGGATGAGCTTGATTTCCACGCCGATGACAGGAAAA
  CTACAAGCAAGTTAGGCTCCGACGTCCAAGTCCAAGCCACTGCGTGTGTTCTCTCTCTGTGCGGCTGGGC
  GTGTAGTTCCTTGGAACCTACTCAGCTCTCCCTGATAACATGTTACCAGTGTATGAGGAAGGTCGGTCTC
  TGGGGCTTTCAGCAGATTGAATCATCCATGACAGATCTGGAGGCTTCCTTTGGCCTGACCAGCTCCCCGA
  TTCCAGGTGTGGAGGGCCGGCCTGAACACTTCCCTCTGGTGCCTGAATCCCCTAGGCGGATGATGACCCG
  AAGCCAGGATGCTACAGTTTCTCCAGGCTCTGAGCAGTCTGAAAAAAGTCCTGGTCCTATTGTTTCCCGA
  ACCCGGAGTTGGGAGTCTTCCAGTCCTGTTGACCGGCCTGAGCTAGAGGCAGCCAGTCCTACTACCAGGA
  GCCGCCCAGTGACCCGAAGCATGGGAACAGGAGATTCTGCTGGCGTGGAAGTTCCCTCGAGCCCTCTCCG
  GAGAACCAAGCGAGCTCGCCTCTGCTCTTCCAGCAGCTCTGACACCTCTCCCCGAAGCTTCTTTGATCCC
  ACCTCTCAGCATAGAGATTGGTGCCCGTGGGTGAATATCACACTCGTCAAAGAAACCAAGGAGAATGGGG
  AAACAGAAGTGGATGCCTGTACCCCAGCAGAGCCAGGTTGGAAGGCAGTGTTGACCATCCTGTTGGCCCA
  CAAGCGTTCTAACCAGCCAGCTGAGACAGACTCCATGCTGAAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_172735
• Insert Size: 1446 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172735.2, NP_766323.1
• RefSeq Size: 1847 bp
• RefSeq ORF: 1446 bp
• Locus ID: 232679
• UniProt ID: Q80YV2
• Cytogenetics: 6 A3.3
• Gene Summary: Essential component of a SCF-type E3 ligase complex, SCF(NIPA), a complex that controls mitotic entry by mediating ubiquitination and subsequent degradation of cyclin B1 (CCNB1). Its cell-cycle-dependent phosphorylation regulates the assembly of the SCF(NIPA) complex, restricting CCNB1 ubiquitination activity to interphase. Its inactivation results in nuclear accumulation of CCNB1 in interphase and premature mitotic entry (By similarity). Overexpression may be able to protect from apoptosis induced by IL-3 withdrawal.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) contains an alternate exon in the 3' coding region, compared to variant 1. It encodes isoform 2, which has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.