Grsf1 (NM_178700) Mouse Untagged Clone

CAT#: MC216720

Grsf1 (untagged) - Mouse G-rich RNA sequence binding factor 1 (Grsf1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Grsf1
• Synonyms: BB232551; C80280; D5Wsu31e
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216720 representing NM_178700
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC
  GCCC
  
  ATGGCCGGGACGCGCTGGGTGCTAGGCGCGTTGCTCCGGGGCTGCGGCTGCAACTGCAGCAGCTGCCGGC
  GGACCGGCGCCGCCTGCCTGCCTTTCTACTCCGCCGCCGGCACCTTCCCGTCGGGCGTCTCTGGCCGCCG
  CCGCCTGCTGCTACTCCTCGGGGCCGCCGCGGCCGCCGCTTCGCAGACGCGGGGCCTGCAGCTCGGGCCT
  GCGGCCGCCGGGAGGCTGGCGGGTCCCATCCCTGCCCGTCCCTCCGCCGCCGCGGCCGCCGCCGCGTCCT
  ACTCGGCCCTGCGCGCCCCGCTGTTTCCGCGATCGCTGGCAGCCGCCGCGGGCCCGGCGCGGGGTTACAG
  TCAGGAATCCAAAACTACCTACCTGGAAGACCTCCCACCACTGCCTGAGTACGAGTTGAGCCCATCCAAG
  CTAGGAGACGAGGTGGATGATGTTTATCTCATTCGAGCTCAGGGGCTGCCGTGGTCCTGCACTGTGGAAG
  ATGTTCTTAACTTTTTCTCAGACTGCAGAATCCGAAACAGTGAGAATGGAATACATTTCCTCCTAAATAG
  AGATGGGAAACGGAGGGGCGATGCCTTAATAGAGATGGAGTCCGAGCAGGATGTACAGAAGGCCTTAGAA
  AAGCACCGGATGTACATGGGGCAGCGGTATGTGGAAGTGTATGAAATAAACAACGAAGATGTGGATGCCT
  TAATGAAGAGCCTGCAGGTCAAGCCTTCGCCTGTGCTCAGTGATGGTGTGGTCCGCCTGAGAGGACTTCC
  TTACAGCTGCAATGAAAAGGACATTGTGGACTTCTTTGCAGGACTGAACATAGTAGACATTACTTTTGTG
  ATGGACTATAGAGGGAGAAGAAAAACGGGGGAAGCCTATGTACAGTTTGAAGAACCAGAAATGGCCAACC
  AAGCGCTTCTAAAGCACAGGGAAGAAATTGGTAACCGATACATAGAGATATTTCCAAGCAGAAGGAATGA
  AGTTCGAACACATGTTGGATCTCATAAAGGAAAGAAAATGACATCGTCTCCTCCTACTAAATATATAACT
  GAACCAGAAGTGGTATTTGAGGAACATGAAGTAAATGAAGATATTCGGCCTATGACGGCTTTTGAGAGTG
  ATAAAGAGATAGAATTGCCTAAGGAGATGTCAGAAAAACTTCCAGAGGCTGTCGATTTTGGAACTCTGCC
  TTCCCTGCATTTTGTCCACATGAGAGGATTGCCTTTCCAAGCCAATGCCCAAGACATTATAAATTTCTTT
  GCTCCCCTGAAGCCTGTGAGAATCACCATGGAATACAGCTCCAGTGGGAAGGCCACTGGAGAAGCTGACG
  TGCACTTTGATACCCATGAGGATGCTGTTGCAGCCATGCTCAAGGATCGGTCCCATGTCCAACATAGGTA
  TATTGAGCTGTTCCTAAATTCATGTCCTAAAGGAAAATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: AscI-MluI
• ACCN: NM_178700
• Insert Size: 1440 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178700.5, NP_848815.2
• RefSeq Size: 2753 bp
• RefSeq ORF: 1440 bp
• Locus ID: 231413
• UniProt ID: Q8C5Q4
• Cytogenetics: 5 43.85 cM
• Gene Summary: Regulator of post-transcriptional mitochondrial gene expression, required for assembly of the mitochondrial ribosome and for recruitment of mRNA and lncRNA. Binds RNAs containing the 14 base G-rich element. Preferentially binds RNAs transcribed from three contiguous genes on the light strand of mtDNA, the ND6 mRNA, and the long non-coding RNAs for MT-CYB and MT-ND5, each of which contains multiple consensus binding sequences (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.