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Arhgap1 (NM_001145902) Mouse Untagged Clone

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SKU
MC216716
Arhgap1 (untagged) - Mouse Rho GTPase activating protein 1 (Arhgap1), transcript variant 1, (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
Tag Free tGFP DDK
AAV Particle:
DDK
  $503.00
4 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol Arhgap1
Synonyms B230365D05Rik; C76222; Cdc42GAP; p50rhoGAP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>MC216716 representing NM_001145902
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGAAGTGGTTGGGGAGCTGGGGGCCCCCGAGGGGGCGGTTCCCATTCCCATCTCGGAGCCGGATG
TTTGCCGAGCTTTGACAGGCGTGGCAGAGGAAGCATTGCCGGAACGAGCCATGGACCCTCTCTCAGAGCT
GCAAGATGACCTGACGCTGGATGACACCAGCCAAGCTCTGAACCAGTTGAAGCTGGCCTCCATTGATGAG
AAGAACTGGCCTTCTGATGAAATGCCAGACTTCCCCAAGTCAGATGACTCCAAAAGCAGCTCTCCAGAAC
CTGTGACACACCTGAAGTGGGATGACCCTTACTATGACATTGCCCGGCACCAGATTGTGGAAGTAGCCGG
TGATGATAAGTATGGGCGGAAGATCATTGTATTCAGTGCTTGCCGGATGCCCCCCAGCCACCAGCTGGAC
CACAGCAAGCTCTTGGGGTATTTGAAACACACATTGGACCAGTATGTGGAGAGTGACTACACACTCCTGT
ACCTGCACCATGGCCTGACCAGTGACAACAAGCCCTCCCTCAGCTGGCTACGGGATGCCTACCGAGAGTT
TGACCGCAAGTACAAGAAGAACATCAAGGCTCTGTACATCGTGCACCCAACCATGTTCATCAAAACTCTG
CTTATCCTCTTTAAGCCTCTCATCAGTTTCAAGTTTGGACGGAAGATCTTCTACGTGAATTACCTGAGTG
AGCTGAGCGAGCACGTGAAGCTGGAGCAGTTGGGGATCCCTCGCCAAGTGCTCAAGTATGATGACTTCCT
CAAATCCACACAGAAAAGCCCTGCAACAGCTCCCAAGCCCATGCCACCACGGCCCCCCCTGCCCAATCAG
CAGTTTGGGGTCTCCTTACAGCACCTCCAGGAGAAAAGCCCAGGCCAGGACCCCATTCCCATTGTCCTCA
GGGAGACCGTCGCCTACTTGCAGGCTCACGCTCTCACCACTGAGGGCATCTTCCGGAGGTCTGCCAACAC
CCAGGTTGTCCGGGAAGTGCAGCAGAAATACAATATGGGGCTGCCGGTGGACTTTGACCAGTACAACGAG
CTGCACTTGCCCGCTGTCATCCTCAAGACATTCCTTCGGGAGCTTCCTGAACCCTTGCTAACCTTTGACC
TGTACCCCCATGTTGTGGGCTTCCTCAATATTGATGAAAGCCAGAGAGTGGAAGTGACACAGCAGGTTCT
CCAAACTCTGCCAGAGGAGAACTACCAGGTGCTTCATTTCCTGACTGCCTTCCTGGTGCAGATATCTGCC
CACTGTGACCAGAACAAGATGACCAACACTAACTTGGCTGTAGTCTTCGGCCCTAACCTGCTCTGGGCTA
AAGATGCTGCCATCACCCTCAAGGCTATCAATCCCATCAACACCTTCACTAAGTTCCTCCTGGATCATCA
AGGGGAATTGTTCCCCAGCACAGATGCCCAGGGGGTCTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI |
ACCN NM_001145902
Insert Size 1440 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001145902.1, NP_001139374.1
RefSeq Size 2904 bp
RefSeq ORF 1440 bp
Locus ID 228359
UniProt ID Q5FWK3
Cytogenetics 2 E1
Summary GTPase activator for the Rho, Rac and Cdc42 proteins, converting them to the putatively inactive GDP-bound state. Cdc42 seems to be the preferred substrate (By similarity).UniProtKB/Swiss-Prot Function
Transcript Variant: This variant (1) encodes the longer isoform (1).
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Documents
Resources
"NM_001145902" in other vectors (4)
SKU Description Size Price
MG222984 Arhgap1 (tGFP-tagged) - Mouse Rho GTPase activating protein 1 (Arhgap1) transcript variant 1, (10ug) 10 ug
$657.00
MR222984 Arhgap1 (Myc-DDK-tagged) - Mouse Rho GTPase activating protein 1 (Arhgap1), transcript variant 1 10 ug
$289.00 $457.00
MR222984L3 Lenti ORF clone of Arhgap1 (Myc-DDK-tagged) - Mouse Rho GTPase activating protein 1 (Arhgap1), transcript variant 1 10 ug
$757.00
MR222984L4 Lenti ORF clone of Arhgap1 (mGFP-tagged) - Mouse Rho GTPase activating protein 1 (Arhgap1), transcript variant 1 10 ug
$757.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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