Cdc14b (NM_172587) Mouse Untagged Clone

CAT#: MC216713

Cdc14b (untagged) - Mouse CDC14 cell division cycle 14 homolog B (S. cerevisiae) (Cdc14b), transcript variant 1, (10ug)


  "NM_172587" in other vectors (4)

Reconstitution Protocol

USD 503.00

4 Weeks*

Size
    • 10 ug

Product Images

Frequently bought together (3)
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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
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Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Symbol Cdc14b
Synonyms 2810432N10Rik; A530086E13Rik; AA472821; Cdc14B1; CDC14B3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC216713 representing NM_172587
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGCGTAAGAGCGAGCGGCGCTCGGCTTGGGCCACCGCGCCCCCCTGCTCACGCCGCAGCTCGTCCT
CCTCTCCCGGCGTGAAGAAGAGCCGCAGCTCCACGCCGCAGGAGCTGCACCGCCTGGAGCAGCAGGACGA
CCTGTACCTGGACATCACGGATCGCCTTTGTTTTGCCATTCTCTACAGCAGACCAAAGAGTGCAACAAAC
GAACATTATTTCAGCATAGATAACGAGCTAGAGTATGAGAACTTCTATGCGGATTTTGGACCACTCAATC
TGGCCATGGTTTACAGATATTGTTGCAAGATAAATAAGAAGCTCAAGTCCATTACAATGCTGAGGAAGAA
AATTATCCATTTTACTGGCACTGATCAGAGAAAGCAAGCGAATGCCGCCTTCCTTGTCGGGTGTTATATG
GTTATATATTTGGGGAGGACACCAGAAGACGCGTATAGAACATTAATCTTCGGAGATACAGCCTATATTC
CTTTCAGAGATGCTGCCTATGGAAGCTGCAGTTTCTATATTACACTTCTCGACTGTTTTCACGCAGTAAA
GAAGGCAATGCAGTATGGCTTCTTTAATTTCAACTCATTTAACCTTGACGAATATGAACACTATGAAAAA
GCAGAAAATGGAGATTTCAACTGGATAATACCAGAGCGATTTCTTGCCTTCTGTGGGCCCCATTCAAGAT
CCAGGCTGGAAAGTGGTTACCACCAACATTCTCCGGAGACTTATATTCCATATTTTAAGAATCACAATGT
AACTACCATTATTCGTCTGAATAAAAGGATGTATGATGCTAAGCGCTTTACGGATGCTGGATTCGATCAC
CATGATCTTTTCTTTCCGGATGGCAGCACCCCTGCTGAGTCAATTGTCCAAGAATTTCTGGATATTTGTG
AAAATGTCAAGGGTGCCATTGCTGTGCATTGCAAAGCTGGCCTTGGTCGAACAGGCACTCTGATAGGCTG
CTACCTCATGAAGCATTACAGGATGACAGCAGCTGAGAGCATTGCCTGGCTCAGAATCTGTAGACCCGGC
TCAGTAATTGGGCCCCAGCAACAGTTTCTGGTCATGAAACAATCAAGCCTCTGGCTAGAAGGCGACTATT
TCCGTCAAAAGTTAAGAGGGCAGGAGAATGGCCCCCTCAGAGAAGCCTTCTCCAAACACCTTTCGGATGC
TGATGACCTGTCCTTAAATGGGCTTGAGAATCAAGACAATCAAGAGCCTGAGCCGTATAGTGATGATGAT
GAAGTCAGTGGAATGACACAAGGAGACAGACTTCGGGCCCTGAAAAGCCGGAGACAGCCGAAAGCCAGCG
CTATCCCCCTCACATGTCCCCTAGCTGTGCTGACCTCTGCACTGTGTAGTGTTGCCATCTGGTGGATTGT
GTGTGACTACATTCTTCCCACCCTGCTGTTCTGTCTTGATGGCTTCAGAACACAGTAG


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-RsrII     
ACCN NM_172587
Insert Size 1458 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_172587.3, NP_766175.3
RefSeq Size 5859 bp
RefSeq ORF 1458 bp
Locus ID 218294
UniProt ID Q6PFY9
Cytogenetics 13 B3
Gene Summary Dual-specificity phosphatase involved in DNA damage response. Essential regulator of the G2 DNA damage checkpoint: following DNA damage, translocates to the nucleus and dephosphorylates FZR1/CDH1, a key activator of the anaphase promoting complex/cyclosome (APC/C). Dephosphorylates SIRT2 around early anaphase. Dephosphorylation of FZR1/CDH1 activates the APC/C, leading to the ubiquitination of PLK1, preventing entry into mitosis. Preferentially dephosphorylates proteins modified by proline-directed kinases (By similarity).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Other Versions

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.