Alg1 (NM_145362) Mouse Untagged Clone

CAT#: MC216706

Alg1 (untagged) - Mouse asparagine-linked glycosylation 1 homolog (yeast, beta-1,4-mannosyltransferase) (Alg1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Alg1
• Synonyms: HMAT1; HMT1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216706 representing NM_145362
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCGTCCTGCGTGGCGCTGCTGGTTCTGGCGCTGCTGTTGCTGGTGCTGCTGCTGGGCTTGTGGA
  AGCGTGGGCGGCAAACGGGCAGGGCCCGGCACATGGTCGTCGTGGTGCTGGGCGACGTGGGCCGCAGCCC
  CCGCATGCAGTACCACGCGCTGTCATTGGCCCAGAGCGGCTTTTCGGTGACGCTGCTGGGGTTCTACAAC
  TCCAAACCTCGAGATGAACTCTTGCAGAATGATAGGATTCGGATTGTGAAGTTGACAGATCTGCGGGGCC
  TGGGAGCTGGGCCCCGAATTCTCCAGTATGGAGTCAAGGTTGTATTTCAGGCAGTGTACTTGCTGTGGAA
  GATGATGCGCATGGACCCTGCAGCCTACATCTTCCTCCAGAACCCTCCAGGTCTGCCTGCTATTGCCGTC
  TGCTGGTTTGTGGGCTGCATTTGTGGGAGCAAATTGGTCATCGACTGGCACAACTATGGCTACTCCATCA
  TGGGCTTGGTCCATGGCCCCCGCCACCCCATTGTGCTGCTGGCCAAATGGTATGAGAAGTTCTTCGGGCG
  CCTGTCCCACCTGAACCTGTGTGTGACCAATGCTATGCGGGAGGACCTGGCAGAGAACTGGTGTGTCAGG
  GCTGTGACGCTCTACGACAAGCCAGCATCTTTCTTTAAGGAGACACCCCTGGACCTGCAGCATGAACTCT
  TTATGAAGCTGAGCCACACGTACTCTCCTTTCCAGAGCTGCTCAGATCCCTCACATCCTGACACAGAGAG
  GTCGGCCTTCACTGAGAGGGATTGTCAGAGCGGGGTTGTGAGGCGTCTGCATGGGCGGCCAGCACTGCTC
  GTGAGCAGCACAAGCTGGACAGAGGACGAGGACTTCTCCATCCTGCTTCGAGCATTAGAAAAGTTTGAGC
  AACAAGCACTCACTGGAGACAGCCTCCCTTCCCTCGTCTGTGTGATAACAGGGAAGGGACCGCTCAGAGA
  GCACTACCGCCACCTCATCAGCCAGAAGCACCTTCAGCACGTCCGGTTCTGCACCCCATGGCTGGAAGCC
  GAGGACTACCCACTGCTTCTAGGGTCAGCGGATCTGGGTGTCTGTCTACACATGTCCTCCAGTGGTCTGG
  ACCTGCCCATGAAGGTGGTGGACATGTTTGGGTGCCACTTGCCTGTGTGTGCCGTGAACTTCAAGTGTCT
  GCACGAGCTAGTAAGACATGGAGAGAATGGTCTGGTCTTCAAGGATGCTGAGGAGCTGGCAGCTCAGCTG
  CAGATGCTTTTCTCAAAGTTCCCAGACCCTGCTGGAAAGCTAAGCCAGTTCCGGAAGAAACTGCAGGAGT
  CGGGGCAGCAACGCTGGGATGAGAGCTGGCAGCACACTGTGCTCCCTCTGCTCGCTCACTCACAGATGAC
  CCCCAGGCCCCATCCACCCTGTGGCCACCCTTCCTGCCGGGGCTTTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_145362
• Insert Size: 1449 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145362.2, NP_663337.2
• RefSeq Size: 1772 bp
• RefSeq ORF: 1449 bp
• Locus ID: 208211
• UniProt ID: Q921Q3
• Cytogenetics: 16 A1
• Gene Summary: Participates in the formation of the lipid-linked precursor oligosaccharide for N-glycosylation. Involved in assembling the dolichol-pyrophosphate-GlcNAc(2)-Man(5) intermediate on the cytoplasmic surface of the ER (By similarity).[UniProtKB/Swiss-Prot Function]