Dnaja3 (NM_023646) Mouse Untagged Clone

CAT#: MC216696

Dnaja3 (untagged) - Mouse DnaJ (Hsp40) homolog, subfamily A, member 3 (Dnaja3), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Dnaja3
• Synonyms: 1200003J13Rik; 1810053A11Rik; C81173; Tid-1; Tid1l
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216696 representing NM_023646
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCGTGGTGTTCCCCACGCTGGTTGAGGGTGGCAGTGGGGACTCCCCGGCTGCCGGCTGCAGCGG
  GGAGAGGGGTCCAGCAGCCCCAGGGGGGCGTAGTGGCAACATCCCTGTGTCGCAAGCTGTGCGTCTCTGC
  CTTCGGGCTTTCCATGGGCGCTCACGGCCCCCGGGCGCTGCTGACATTGAGACCCGGTGTCCGCCTCACA
  GGAACAAAAAGTTTCCCTTTTGTTTGTACCACCTCTTTCCACACAAGTGCTTCTTTGGCCAAAGATGATT
  ATTACCAGATCCTGGGAGTGCCCCGAAATGCCAGCCAGAAAGATATCAAGAAAGCCTATTATCAGCTTGC
  CAAGAAATATCATCCAGACACAAATAAGGATGATCCCAAAGCCAAGGAGAAGTTTTCCCAGCTGGCAGAA
  GCTTATGAGGTGTTGAGCGATGAAGTGAAGAGGAAGCAGTATGACGCTTACGGCTCTGCTGGCTTTGACC
  CTGGCACCAGCAGCTCTGGGCAGGGCTACTGGAGAGGAGGTCCCTCTGTTGACCCCGAGGAGTTGTTCAG
  GAAGATCTTTGGGGAGTTCTCATCTTCTCCTTTTGGTGATTTCCAGAATGTGTTTGATCAGCCTCAGGAA
  TACATCATGGAATTGACATTCAATCAAGCTGCCAAGGGTGTCAACAAAGAGTTCACTGTGAACATTATGG
  ATACCTGTGAGCGCTGTGACGGCAAGGGGAACGAGCCTGGAACCAAAGTGCAGCATTGCCATTACTGTGG
  CGGCTCGGGCATGGAAACTATCAATACAGGGCCTTTTGTGATGCGTTCCACATGTCGGAGATGTGGTGGC
  CGGGGCTCCATCATCACAAATCCCTGTGTGGTCTGCAGGGGAGCAGGACAAGCCAAGCAGAAGAAGCGCG
  TGACAATTCCTGTGCCTGCAGGAGTTGAAGATGGTCAGACTGTAAGGATGCCAGTGGGAAAACGAGAAAT
  TTTTGTCACATTCAGGGTGCAGAAAAGCCCTGTGTTCCGGAGGGACGGTGCAGACATCCACTCGGACCTC
  TTTATTTCAATAGCTCAAGCTATTCTTGGGGGCACAGCCAAAGCCCAGGGCCTGTATGAGACAATCAATG
  TGACGATCCCTGCAGGGATTCAGACAGACCAGAAGATTCGTTTGACTGGAAAAGGCATCCCCCGGATTAA
  TAGCTATGGCTATGGTGACCACTACATTCACATCAAGATCAGAGTTCCAAAGAGGCTGAGCAGTAGGCAG
  CAGAACCTGATCCTGAGCTATGCCGAGGATGAGACTGACGTGGAGGGGACGGTTAATGGAGTCACCCACA
  CAAGCACTGGTGGCAGAACCATGGATAGCTCCGCAGGAAGCAAGGATAGGCGAGAGGCTGGGGAGGACAA
  TGAGGGATTCCTTTCCAAACTTAAGAAAATATTTACTTCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_023646
• Insert Size: 1443 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023646.4, NP_076135.3
• RefSeq Size: 2666 bp
• RefSeq ORF: 1443 bp
• Locus ID: 83945
• UniProt ID: Q99M87
• Cytogenetics: 16 2.46 cM
• Gene Summary: Modulates apoptotic signal transduction or effector structures within the mitochondrial matrix. Affect cytochrome C release from the mitochondria and caspase 3 activation, but not caspase 8 activation. Isoform 1 increases apoptosis triggered by both TNF and the DNA-damaging agent mytomycin C; in sharp contrast, isoform 2 suppresses apoptosis. Can modulate IFN-gamma-mediated transcriptional activity (By similarity). Isoform 2 may play a role in neuromuscular junction development as an effector of the MUSK signaling pathway.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (1).