Syn2 (NM_013681) Mouse Untagged Clone

CAT#: MC216649

Syn2 (untagged) - Mouse synapsin II (Syn2), transcript variant IIb, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Syn2
• Synonyms: 2900074L19Rik; AI836018; AI841723
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216649 representing NM_013681
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGAACTTCCTGAGGCGCCGGCTGTCGGACAGCAGCTTCATCGCCAACCTGCCCAATGGCTACATGA
  CTGACCTTCAGCGCCCGGAGCCGCAGCAGCCCCCTCCTGCCCCTGGCCCCGGTGCCGCTACGGCGTCGGC
  GGCCACCTCAGCTGCCTCGCCCGGTCCCGAGCGCAGGCCGCCACCCGCCCAGGCACCCGCGCCGCAGCCG
  GCGCCGCAGCCCGCGCCGACGCCGTCGGTGGGCAGCAGCTTCTTCAGCTCGCTGTCGCAAGCGGTGAAGC
  AGACGGCCGCCTCCGCCGGCCTGGTGGACGCGCCCGCACCTTCGGCCGCTTCCAGGAAGGCCAAGGTGCT
  GCTGGTGGTCGACGAGCCGCACACCGACTGGGCCAAATGCTTTCGGGGCAAAAAAATCCTTGGAGATTAT
  GACATCAAGGTGGAACAGGCAGAATTCTCAGAGCTTAACCTGGTGGCCCATGCAGATGGAACCTACGCCG
  TGGACATGCAGGTACTTCGGAATGGCACAAAGGTTGTCCGGTCCTTCAGACCAGACTTTGTGCTCATCCG
  ACAGCATGCATTTGGCATGGCAGAGAATGAAGACTTCCGCCACCTGGTCATTGGCATGCAGTACGCAGGC
  CTCCCCAGCATCAACTCTCTGGAGTCTATTTACAACTTCTGTGACAAGCCGTGGGTGTTTGCTCAGATGG
  TGGCCATCTTCAAGACACTGGGAGGGGAGAAATTCCCACTCATTGAGCAGACATACTACCCCAATCACCG
  AGAGATGCTTACGCTTCCTACGTTCCCTGTGGTGGTGAAGATTGGCCATGCTCACTCAGGCATGGGCAAG
  GTCAAAGTGGAAAATCACTACGACTTCCAGGACATTGCCAGTGTGGTGGCCCTCACCCAAACTTATGCCA
  CGGCAGAACCCTTCATCGACGCCAAGTATGACATCCGAGTTCAGAAGATTGGCAACAACTACAAGGCCTA
  CATGAGGACATCCATCTCAGGGAACTGGAAGACAAACACTGGCTCGGCGATGCTGGAGCAAATTGCCATG
  TCGGACCGGTACAAGCTCTGGGTAGATGCCTGCTCTGAAATGTTTGGTGGCCTGGACATCTGTGCAGTCA
  AAGCCGTGCATGGCAAAGATGGCAAAGACTACATTTTTGAGGTCATGGACTGCAGCATGCCATTGATTGG
  GGAACACCAAGTGGAGGACAGACAACTCATCACTGATCTAGTCATCAGCAAGATGAACCAGCTATTGTCC
  AGGACTCCTGCCCTGTCTCCACAGAGACCTTTAACCACCCAACAGCCACAGAGTGGAACACTTAAGGAAC
  CGGACTCCAGCAAGACCCCTCCTCAGCGGCCACCTCCCCAAGGTTGTTTACAGTATATTCTCGACTGTAA
  TGGCATTGCAGTAGGTCCAAAACAAGTCCAAGCTTCTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_013681
• Insert Size: 1440 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013681.3, NP_038709.1
• RefSeq Size: 3858 bp
• RefSeq ORF: 1440 bp
• Locus ID: 20965
• UniProt ID: Q64332
• Cytogenetics: 6 53.2 cM
• Gene Summary: Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. May play a role in noradrenaline secretion by sympathetic neurons.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (IIb) lacks two exons and its 3' terminal exon extends past a splice site that is used in variant IIa. This results in a novel 3' coding region and 3' UTR, compared to variant IIa. It encodes isoform IIb which is shorter and has a distinct C-terminus, compared to isoform IIa.