Pou2f2 (NM_001163555) Mouse Untagged Clone

CAT#: MC216644

Pou2f2 (untagged) - Mouse POU domain, class 2, transcription factor 2 (Pou2f2), transcript variant 3, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pou2f2
• Synonyms: Oct-2; Oct2a; Oct2b; Oct2c; Oct2d; Otf-2; Otf2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NM_001163555.1
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTCATTCCAGCATGGGGGCTCCAGAAATAAGAATGTCTAAGCCCCTGGAGGCCGAGAAGCAAAGTC
  TGGACTCCCCGTCAGAGCACACAGACACCGAAAGAAATGGACCCGACATTAACCATCAGAACCCCCAGAA
  TAAAGCGTCCCCATTCTCTGTGTCCCCAACTGGCCCCAGCACCAAGGTGGGCATTCTCTCTGGCCTCCAC
  TTAACATTCTGGGGTCCCGGACCCTGCCTCTCTCCTCCCCAGATCAAGGCTGAAGACCCCAGTGGCGATT
  CAGCCCCAGCAGCACCCCCGCCCCCCCAGCCGGCTCAGCCTCATCTGCCCCAGGCCCAACTCATGCTGAC
  GGGCAGCCAGCTAGCTGGGGACATACAGCAACTCCTCCAGCTCCAGCAGCTGGTGCTTGTCCCCGGCCAC
  CACCTCCAGCCACCTGCTCAGTTCCTGCTGCCACAGGCACAGCAGAGTCAGCCAGGCCTGCTACCAACGC
  CAAATCTATTCCAGCTACCTCAACAAACCCAGGGAGCTCTCCTGACCTCCCAGCCCCGGGCTGGGCTTCC
  TACACAGCCCCCGAAATGCTTGGAGCCGCCCTCCCACCCGGAGGAGCCCAGCGATCTGGAGGAGCTGGAA
  CAGTTTGCTCGCACCTTCAAGCAACGCCGCATCAAGCTGGGCTTCACACAGGGTGATGTGGGCCTGGCCA
  TGGGCAAGCTCTATGGCAACGACTTCAGCCAAACGACCATTTCCCGCTTCGAGGCCCTCAACCTGAGCTT
  CAAGAACATGTGTAAACTCAAGCCCCTCCTGGAGAAGTGGCTCAACGACGCAGAGACTATGTCTGTGGAT
  TCAAGCCTACCCAGCCCAAACCAGCTGAGCAGCCCCAGCCTGGGTTTCGACGGGCTGCCGGGGCGGAGAC
  GCAAGAAGAGGACCAGCATCGAGACGAATGTCCGCTTCGCCTTAGAGAAGAGTTTCCTAGCGAACCAGAA
  GCCTACCTCAGAGGAGATCCTGCTGATCGCAGAGCAGCTGCACATGGAGAAGGAAGTGATCCGCGTCTGG
  TTCTGCAACCGGCGCCAGAAGGAGAAACGCATCAACCCTTGCAGTGCGGCCCCCATGCTGCCCAGCCCGG
  GAAAGCCGACCAGCTACAGCCCTCACCTGGTCACACCCCAAGGGGGCGCAGGGACCTTACCATTGTCCCA
  AGCTTCTAGCAGTCTGAGCACAACAGTTACTACCTTATCCTCAGCTGTGGGGACGCTCCATCCCAGCCGG
  ACAGCAGGAGGGGGTGGGGGTGGGGGCGGAGCTGCGCCCCCCCTCAATTCCATCCCCTCTGTCACTCCCC
  CACCCCCGGCCACCACCAACAGCACAAACCCGAGCCCTCAAGGCAGCCACTCGGCTATTGGCTTGTCGGG
  CCTGAACCCCAGCGCGGGCCCTGGCCTCTGGTGGAACCCTGCCCCTTACCAGCCTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001163555
• Insert Size: 1458 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163555.1, NP_001157027.1
• RefSeq Size: 3315 bp
• RefSeq ORF: 1458 bp
• Locus ID: 18987
• UniProt ID: Q00196
• Cytogenetics: 7 13.73 cM
• Gene Summary: Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. Modulates transcription transactivation by NR3C1, AR and PGR. Isoform OCT2.5 activates the U2 small nuclear RNA (snRNA) promoter. Isoforms OCT2.1, OCT2.2 and OCT2.3 activate octamer-containing promoters. Isoforms OCT2.4 and OCT2.5 repress some promoters and activate others. Isoform OCT2.7 is unable to bind to the octamer motif, but can still activate the beta-casein gene promoter at low levels.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) has an additional segment in the coding region, as compared to variant 1. The resulting isoform (3) is longer and has the same N- and C-termini, as compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.