Pax3 (NM_008781) Mouse Untagged Clone

CAT#: MC216638

Pax3 (untagged) - Mouse paired box gene 3 (Pax3), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Pax3
• Synonyms: Pax-3; Sp; Splchl2; splotch
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216638 representing NM_008781
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCACGCTGGCCGGCGCTGTGCCCAGGATGATGCGGCCCGGCCCGGGGCAGAATTACCCACGCAGCG
  GCTTCCCGCTGGAAGTGTCCACCCCTCTTGGCCAGGGCCGAGTCAACCAGCTCGGAGGAGTATTTATCAA
  CGGCAGGCCTCTGCCCAACCATATCCGCCACAAGATAGTGGAGATGGCCCACCATGGCATTCGGCCTTGC
  GTCATTTCTCGCCAGCTTCGCGTGTCCCATGGTTGCGTCTCTAAGATCCTGTGCAGGTACCAGGAGACAG
  GCTCCATCCGACCTGGTGCCATCGGCGGCAGCAAACCCAAGCAGGTGACAACGCCTGACGTGGAGAAGAA
  AATTGAGGAATACAAAAGAGAGAACCCGGGCATGTTTAGCTGGGAAATCAGAGACAAATTGCTCAAGGAC
  GCTGTCTGTGATCGGAACACTGTGCCCTCAGTGAGTTCTATCAGCCGCATCCTGAGGAGTAAATTTGGAA
  AAGGAGAAGAGGAGGAGGCGGATCTAGAAAGGAAGGAAGCAGAAGAAAGCGAGAAAAAGGCTAAACACAG
  CATCGATGGCATCCTGAGTGAGCGAGCCTCTGCACCTCAGTCAGATGAAGGCTCCGATATTGACTCTGAA
  CCTGATTTACCGCTGAAGAGGAAGCAGCGCAGGAGCAGAACCACCTTCACGGCAGAGCAGCTGGAGGAAC
  TGGAGCGGGCTTTCGAGAGAACCCACTACCCAGACATTTACACCAGGGAGGAGCTGGCCCAGAGGGCGAA
  GCTTACCGAGGCCCGAGTGCAGGTCTGGTTTAGCAACCGCCGTGCAAGATGGAGGAAACAAGCTGGAGCC
  AATCAACTGATGGCTTTCAACCATCTCATTCCGGGGGGATTCCCTCCCACCGCCATGCCGACCCTGCCAA
  CATACCAGCTGTCGGAGACCTCTTACCAGCCCACGTCTATTCCACAAGCCGTGTCAGATCCCAGTAGCAC
  CGTCCACAGACCTCAGCCGCTTCCTCCGAGCACTGTACACCAAAGCACTATTCCTTCGAACGCAGACAGC
  AGCTCTGCCTACTGCCTCCCCAGCACCAGGCATGGATTTTCAAGCTATACAGACAGCTTTGTGCCTCCAT
  CGGGGCCCTCCAACCCCATGAACCCCACCATCGGCAATGGCCTTTCACCTCAGGTAATGGGACTTCTGAC
  CAACCACGGTGGGGTACCGCACCAGCCTCAGACCGACTATGCTCTCTCCCCTCTCACTGGGGGCCTGGAA
  CCCACGACCACGGTGTCAGCCAGCTGCAGTCAGAGACTGGAACATATGAAGAATGTGGACAGTCTGCCCA
  CATCTCAGCCCTATTGTCCCCCCACCTATAGCACCGCAGGCTACAGTATGGACCCTGTCACAGGCTACCA
  GTATGGGCAGTATGGACAAAGTAAGCCTTGGACGTTCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008781
• Insert Size: 1440 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008781.4, NP_032807.3
• RefSeq Size: 3868 bp
• RefSeq ORF: 1440 bp
• Locus ID: 18505
• UniProt ID: P24610
• Cytogenetics: 1 39.79 cM
• Gene Summary: Transcription factor that may regulate cell proliferation, migration and apoptosis. Involved in neural development and myogenesis. Transcriptional activator of MITF, acting synergistically with SOX10 (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript but encodes the shorter isoform (a).