Poc1b (NM_027740) Mouse Untagged Clone

CAT#: MC216579

Poc1b (untagged) - Mouse POC1 centriolar protein homolog B (Chlamydomonas) (Poc1b), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Poc1b
• Synonyms: 4933430F16Rik; Wdr51b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216579 representing NM_027740
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCCGGCCTGGAGGATCCGATTCTGGAACGTTCTTTTAAAGGTCACAAAGCTGCCATCACCTCCG
  CTGACTTCAGCCCCAACTGCAAACAAATTGCTACTGCTTCGTGGGACACCTTTCTCATGCTATGGAGTTT
  GAAGCCACACGCTAGAGCTTACAGATACGTGGGCCACAAGGATGTTGTAACCAGCCTGCAGTTCTCGCCA
  CAAGGAAACCTATTAGCCTCTGCCTCGAGGGACAGAACTGTTAGACTGTGGGTTCTTGATAGGAAAGGGA
  AGTCTTCAGAATTTAAAGCACACACAGCCCCAGTTCGGAGTGTGGACTTTTCCGCTGATGGCCAGCTTTT
  GGTTACAGCATCTGAAGATAAATCCATTAAAGTGTGGAGTATGTTCCGTCAGCGCTTCCTCTACTCCCTG
  TATCGACACACACACTGGGTACGCTGCGCCAAATTTTCACCTGACGGAAGGTTGATCGTGTCATGTAGTG
  AGGATAAAACTATTAAAATTTGGGACACTACCAATAAGCAGTGCGTTAATAACTTCTCAGACTCTGTAGG
  ATTTGCAAATTTTGTGGATTTTAACCCCAACGGCACATGCATCGCTTCAGCAGGTTCTGACCATGCTGTG
  AAAATCTGGGATATAAGAATGAACAAATTACTCCAGCACTACCAAGTTCACAGCTGCGGTGTGAACTGTC
  TGTCCTTCCATCCTTTGGGTAACAGCCTTGTCACCGCCTCTTCTGATGGAACAGTTAAGATGCTGGATCT
  CATAGAGGGAAGACTCATATACACACTTCAAGGACATACGGGGCCCGTCTTTACCGTTTCATTTTCGAAG
  GACGGAGAGCTGCTTACATCAGGCGGGGCAGATGCGCAGGTCTTGATTTGGAGGACCAACTTCATTCACC
  TGCACTGTAAAGATCCTAAAAGAAACCTCAAAAGACTGCACTTTGAGGCCTCCCCACACCTTCTTGACAT
  CTACCCACGGTCACCGCATTCCCATGAAGACAGAAAGGAGACTATTGAAATTAATCCCAAGCGTGAGGTG
  ATGGATTTGCAGAGCTCGTCTCCCCCCGTGGTGGATGTCCTTTCTTTCGATTCAACCACAATGACGGATT
  CCACCTATCGAGCTGTGCCAGGCAAGGGTGAAGACATCTGTAGGTATTTCTTGAACCCTTTGTTAATGCC
  AGAATGTTCATCCACAACTGTGAAAAAGAGACCAGAAGATGTAAGTGATGTGCCCTCCGAAAGCCTGAGG
  AGCGTCCCGCTGGCGGTGGCTGACGCACTGGAGCACATTATGGAACAACTCAACATCCTAACCCAGACTG
  TTTCAATTCTGGAGCAGCGACTGTCTTTGACAGAAGATAAGCTGAGAGACTGCCTTGAGAATCAGCAAAA
  GCTTTTCAGCGCTGTCCAACAGAAGAGCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_027740
• Insert Size: 1431 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027740.6, NP_082016.1
• RefSeq Size: 2727 bp
• RefSeq ORF: 1431 bp
• Locus ID: 382406
• UniProt ID: Q8BHD1
• Cytogenetics: 10 C3- D1
• Gene Summary: Plays an important role in centriole assembly and/or stability and ciliogenesis. Involved in early steps of centriole duplication, as well as in the later steps of centriole length control. Acts in concert with POC1A to ensure centriole integrity and proper mitotic spindle formation. Required for primary cilia formation, ciliary length and also cell proliferation. Required for retinal integrity.[UniProtKB/Swiss-Prot Function]