Kremen1 (NM_032396) Mouse Untagged Clone

CAT#: MC216539

Kremen1 (untagged) - Mouse kringle containing transmembrane protein 1 (Kremen1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kremen1
• Synonyms: AV002070; Kremen; Krm1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216539 representing NM_032396
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCCGCCCGCCGCCCGTCTCGCGCTGCTCTCCGCCGCTGCGCTCACTCTGGCGGCCCGGCCCGCGC
  CCGGTCCCCGCTCCGGCCCCGAGTGCTTCACAGCCAACGGTGCAGATTACAGGGGAACACAGAGCTGGAC
  AGCGCTGCAAGGTGGGAAGCCATGTCTGTTCTGGAACGAGACTTTCCAGCATCCGTACAACACGCTGAAG
  TACCCCAACGGGGAAGGAGGACTGGGCGAGCACAATTATTGCAGAAATCCAGATGGAGACGTGAGCCCTT
  GGTGCTACGTGGCCGAGCATGAGGACGGAGTCTACTGGAAGTACTGTGAAATTCCTGCCTGCCAGATGCC
  TGGAAACCTTGGCTGCTACAAGGATCATGGAAACCCACCTCCTCTCACGGGCACCAGTAAAACCTCTAAC
  AAGCTCACCATACAAACCTGTATCAGCTTCTGTCGGAGTCAGAGATTCAAGTTTGCTGGGATGGAGTCAG
  GCTATGCCTGCTTCTGTGGGAACAATCCTGACTACTGGAAGCACGGGGAGGCGGCCAGCACCGAGTGCAA
  TAGTGTCTGCTTCGGGGACCACACGCAGCCCTGCGGTGGGGACGGCAGGATTATCCTCTTTGACACTCTC
  GTGGGCGCCTGCGGTGGGAACTACTCAGCCATGGCAGCCGTGGTGTACTCCCCTGACTTCCCTGACACCT
  ACGCCACTGGCAGAGTCTGCTACTGGACCATCCGGGTTCCAGGAGCCTCTCGCATCCATTTCAACTTCAC
  CCTTTTTGATATCAGGGACTCTGCAGACATGGTGGAGCTGCTGGACGGCTACACCCACCGCGTCCTGGTC
  CGGCTCAGTGGGAGGAGCCGCCCGCCTCTGTCTTTCAATGTCTCTCTGGATTTTGTCATTTTGTATTTCT
  TCTCTGATCGCATCAATCAGGCCCAGGGATTTGCTGTGTTGTACCAAGCCACCAAGGAGGAACCGCCACA
  GGAGAGACCTGCTGTCAACCAGACCCTGGCAGAGGTGATCACCGAGCAAGCCAACCTCAGTGTCAGCGCT
  GCCCACTCCTCCAAAGTCCTCTATGTCATCACCCCCAGCCCCAGCCACCCTCCGCAGACTGCCCCAGGTA
  GCCATTCCTGGGCACCGTCAGTTGGGGCCAACAGCCACAGAGTGGAAGGATGGACTGTGTACGGCCTGGC
  GACCCTCCTCATCCTCACAGTCACAGCAGTTGTCGCAAAGATTCTTCTGCATGTCACATTTAAATCTCAT
  CGAGTCCCTGCATCAGGAGACCTTAGGGACTGTCGTCAGCCTGGGGCTTCTGGAGATATCTGGACCATTT
  TCTATGAACCTTCCACTACAATCTCCATCTTTAAGAAGAAGCTCAAGGGTCAGAGTCAACAAGATGACCG
  CAATCCCCTCGTGAGTGACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_032396
• Insert Size: 1422 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC082546, AAH82546
• RefSeq Size: 4943 bp
• RefSeq ORF: 1422 bp
• Locus ID: 84035
• UniProt ID: Q99N43
• Cytogenetics: 11 A1
• Gene Summary: Receptor for Dickkopf proteins. Cooperates with DKK1/2 to inhibit Wnt/beta-catenin signaling by promoting the endocytosis of Wnt receptors LRP5 and LRP6 (PubMed:12050670). In the absence of DKK1, potentiates Wnt-beta-catenin signaling by maintaining LRP5 or LRP6 at the cell membrane (By similarity). Can trigger apoptosis in a Wnt-independent manner and this apoptotic activity is inhibited upon binding of the ligand DKK1 (PubMed:26206087). Plays a role in limb development; attenuates Wnt signaling in the developing limb to allow normal limb patterning and can also negatively regulate bone formation (PubMed:18505822). Modulates cell fate decisions in the developing cochlea with an inhibitory role in hair cell fate specification (PubMed:27550540).[UniProtKB/Swiss-Prot Function]