Mfsd12 (NM_028657) Mouse Untagged Clone

CAT#: MC216523

Mfsd12 (untagged) - Mouse RIKEN cDNA F630110N24 gene (F630110N24Rik), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mfsd12
• Synonyms: C19orf28; F630110N24Rik; gr; Wdt1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216523 representing NM_028657
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGCCTCCGTCGGACGACGCAGGCCCGGGGCCGCCGCGGACGCTGTCCCTCGCCGCGCGGCTGAGCT
  TCGCGGTCGGCCACTTCCTCAACGACCTGTGCGCCGGCATGTGGTTCACCTACCTGCTACTATTCCTGCA
  CTCCGTGCGCGGGTACAGCTCGCGGGGCGCGGGGCTGCTGCTGCTGCTGGGCCAAGTGGCCGACGGGCTC
  TGCACGCCGCTCGTGGGCTACGAGGCGGACCGCGCCTCCTGCGTGCGCTGTGGGCCCCGCAAGGCCTGGC
  ACCTAGCGGGCACTGTCTGTGTCCTGCTCTCCTTCCCGTTCATCTTCAGCCCGTGCCTGGGCTGCGGGGA
  AGCCACGCCTGAATGGGCTGCCTTGCTGTACTATGGACCCTTCATTGTCGTCTTCCAGTTTGGCTGGGCT
  GCCACGCAGATTGCCCACCTGAGCCTCATCCCAGAGCTGGTGACCAGTGACCACGAGAAGGTGGAGCTCA
  CGGCCCTCAGGTATGCGTTCACTGTGGTGGCTAACATCACCGTGTACGGCGCAGCCTGGCTGCTGCTGCA
  CTTGCAGGGCTCTGCACACGGCGAGCAGGACATCAGTGTGGGTGACCAGCTAGGAGTCCAGGATGTGCCC
  GTGTTCCGGAACCTGGCCCTGCTTGTGGTGGGTGTGGGAGCCATCTTCTCTCTGCTCTTCCACTTGGGTA
  CCAAGGAGGGCCACAGGTCCCAGCACTGGGGGAACGAACCCAATGAGCACACCCCCCTGGTGGCCCCAGC
  GGCCCAGCCACTGCTGCTGTGGAAACACTGGCTTCGGGAGCCAGCTTTTTATCAGGTAGGCATGCTGTAC
  ATGACCACAAGGCTCATTGTGAACCTGTCTCAGACCTACATTGCCATGTACCTGACCTATTCTCTCAGCT
  TGCCCAAGAAGTTCATCGCCACCATCCCCCTGGTGATGTACTTGAGTGGCTTCTTCTCTTCCTTCCTCAT
  GAAGCCAGTCAACCGGCGCATAGGGAGGAATATGACCTACTTCACCGGGCTGCTGGTGATCCTGGCCTTC
  GCAGCTTGGGTGGCCCTGGCAGATAATCTGGGTGTGGCCGTGTACGGGGCTGCAGTGCTGCTAGGTGCAG
  GCTGTGCCACCATCCTTGTCACCTCCCTAGCCATGACAGCTGACCTCATTGGCCCACACACGCACAGTGG
  AGCCTTCGTGTATGGTGCTATGAGCTTTTCGGATAAGGTGGCCAACGGACTGGCAGTCATGGCTGTGCAG
  AGCCTGCATCCCTGTCCCTCTGAGCTGTGCTGTGGGGCCTGCATCAGCTTCTACCACTGGGTGATGACAG
  CAGTGACAGGCGGCGTGGGTGTGGCCGCGGCTCTGGCTCTCTGCAGCCTCCTCATCTGGCCCATCCGCAT
  TCGCAACCGGGACCCGCGAGACCGGCCTTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_028657
• Insert Size: 1431 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028657.3, NP_082933.2
• RefSeq Size: 1702 bp
• RefSeq ORF: 1431 bp
• Locus ID: 73822
• UniProt ID: Q3U481
• Cytogenetics: 10 C1
• Gene Summary: Transporter that mediates the import of cysteine into melanosomes, thereby regulating skin/hair pigmentation (PubMed:33208952). In melanosomes, cysteine import is required both for normal levels of cystine, the oxidized dimer of cysteine, and provide cysteine for the production of the cysteinyldopas used in pheomelanin synthesis, thereby regulating skin/hair pigmentation (PubMed:33208952). Also catalyzes import of cysteine into lysosomes in non-pigmented cells (By similarity).[UniProtKB/Swiss-Prot Function]