Cpvl (NM_027749) Mouse Untagged Clone

CAT#: MC216514

Cpvl (untagged) - Mouse carboxypeptidase, vitellogenic-like (Cpvl), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cpvl
• Synonyms: 4933436L16Rik; HVLP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216514 representing NM_027749
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTCGTGCCAAGTGGAAGATGGTTGTTTCACTGATCTTGTTTATGGTCAGCCCTGGTGATGGACTGT
  TTCATGCAGTATACCGAAGTATCCTTGTTTCCCAGTCCTTCAAGGGGGATGCAGGACAGCCTCTTTTTCT
  TAGCCCATACATCAAAAACGGGAAGATCAAGGAGGGGCAAAGGAAGAGCATGGTCAGTCCGTTCCCTGGA
  ATGAACGACAAGAGTTATGCCGGCTACATCACGGTGAACCAGACTTACAACAGCAACCTCTTCTTCTGGT
  TCTTTCCGGCTCGGATGCAGCCTGAGGATGCCCCAGTAGTCCTCTGGCTTCAGGGTGGACCTGGAGGTTC
  ATCCATGTTTGGACTCTTTGTAGAACACGGGCCTTATATTATCACAAGTAACATGACCGTTGTGGCCAGA
  GACTTCCCTTGGACCTTTACCCTTTCCATGCTCTACATTGATAATCCGGTGGGTACAGGCTTCAGCTTCA
  CCGATCATTTCCAGGGATACGCCACCAGCGAGGATGATGTAGCCCAAGACTTGTACAGTGCCCTGATTCA
  GTTCTTCACGTTGTTTCCCGAGTATGCAAAGAATGATTTTTACGTCACTGGCGAGTCTTATGCAGGGAAA
  TATGTTCCGGCCCTAGCACACTATATCCACTCCCTCAACCCCGTGCGGAAGTTCAAGATTCGCCTAAAAG
  GAATTGCCATTGGAGATGCATACACTGATCCTGAATCGATTATAGGAGGGTATGCGGCATTCCTATATGA
  AATTGGCTTGTTGGATGAACAGCAGCAGAAGTACTTCCAGAAGCAGTGTAGCAAGTGCGTAAAATACATC
  AAAGAGCAGGAGTGGATGAAGGCCTTTGAAATCCTGGATAAGCTGCTAGATGGAGATGTAACAACTGGTT
  CATCTTTCTTCCAGAATGTGACAGGATGTACCAATTACTACAACATTTTACAGTGCACGGAACCCAAGGA
  GCAAAGTTACTTTGCAAAATTCTTGACGCTCCCCCAAGTGAGACAAGCCATCCACGTGGGAAACCAGAAC
  TTCAGTGACGGTGCTGAGGTTGAGAAGCACCTGCGAGAGGACACAGTGAAGTCGGTGAAGCCCTGGCTGT
  CTGAGATCATGAATTACTACAAGGTTCTCATCTACAATGGCCAACTGGACATCATCGTGGCGGCTGCCCT
  GACAGAGCGCTCCTTGATGGCCATGGACTGGAAGGGATCCCGAGCATACAGAAGGGCACGTCGAAAGGTC
  TGGAAGATCTTCAAATCGGATAATGAAGTGGCTGGTTATGTGAGGCGTGTGGGCAAATTCCATCAGGTAA
  TCGTCCGAGGTGGAGGACACATTTTGCCCTATGACCAGCCAATGAGATCTTTTGATATGATCAATCGCTT
  CATCTATGACAGAGGATGGGAACCTTATAATTCATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_027749
• Insert Size: 1437 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_027749.2, NP_082025.1
• RefSeq Size: 1708 bp
• RefSeq ORF: 1437 bp
• Locus ID: 71287
• UniProt ID: Q9D3S9
• Cytogenetics: 6 B3
• Gene Summary: This gene encodes a member of the serine carboxypeptidase family of proteases that cleave amino acids from the C-terminus of a protein substrate. The human ortholog of this gene, where it was first characterized, was found to be upregulated during the maturation of monocytes to macrophages. The encoded protein may be involved in antigen processing, digestion of phagocytosed proteins in the lysosome and lamellipodium formation. Disruption of this gene in mice was found to cause embryonic lethality. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Variants 1 and 2 encode the same isoform (1).