Mgat4c (NM_001162368) Mouse Untagged Clone

CAT#: MC216504

Mgat4c (untagged) - Mouse mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme C (putative) (Mgat4c), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mgat4c
• Synonyms: 9130411I17Rik; GntIVh
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216504 representing NM_001162368
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTAAATTTTATCAAATGAAATATATTTTTCAAATATTGGATAAAATGAGATGCTTGCGAAAACGGT
  CTACAGTGTCATTCTTGGGGGTTCTTGTTGTTTTTCTACTATTCATGAACTTGTACATTGAAGATAGCTA
  TGTTCTGGAAGGCGACAAGCAACTTATAAGGGAAACATCGACACACCAACTTAATTCTGAACGCTATGTT
  CACACTTTCAAGGATTTATCAAACTTCTCAGGAACCATAAATGTCACTTATCGCTATCTGGCTGCCACAC
  CTTTACAGAGAAAACGATATCTCACAATTGGACTTTCATCAGTGAAACGAAAAAAAGGAAATTATTTACT
  TGACACAATCAAGTCAATTTTTGAACAGTCCAGCTATGAAGAACTAAAGGAAATTTCAGTCGTAGTACAT
  CTAGCAGACTTCAATTCATCATGGCGAGATGCCATGGTCCAAGACATTACACAGAAATTTGCCCATCATA
  TTATTGCAGGAAGATTAATGGTTATACATGCTCCTGAAGAATATTATCCAGTTCTGGATGGTCTTAAAAG
  AAATTACAATGACCCAGAAGATAGAGTCAGATTTCGCTCCAAGCAAAACGTAGATTATGCTTTTCTGCTA
  AATTTCTGTGCCAATACTTCTGACTATTACGTGATGCTTGAAGACGATGTTCGGTGTTCCAGAAATTTCT
  TAACTGCCATCAAGAAAGTCATAGCATCCTTGGAAGGAACATACTGGGTAACTCTTGAGTTCTCTAAACT
  TGGCTACATTGGTAAACTCTATCATTCTCACGATCTCCCACGTCTGGCCCATTTCTTATTAATGTTCTAT
  CAAGAAATGCCCTGCGATTGGCTATTGACTCATTTCCGAGGGCTGCTGGCTCAGAAAAATGTGATTCGAT
  TTAAACCTTCTCTCTTTCAGCACATGGGGTATTATTCATCCTATAAAGGAACAGAGAATAAACTGAAGGA
  TGATGACTTTGAAGAAGAGTCCTTTGACATCCCTGATAACCCCCCAGCGAGTTTCTACACCAACATGAAT
  GTCTTTGAAAACTATGAAGCAAGCAAGGCTTACAGTAGTGTTGATGAGTATTTTTGGGGAAAGTCACCTT
  CAATGGGAGATACGTTCGTTATTGTATTTGAAAATCCGATTACAATTAAAAAAATTAAAGTGAATACTGG
  AACAGAGGACCGGCAGAATGACATCTTACAACATGGAGCCCTAGATGTTGGGGAAAAACTTATTTTTAGC
  AAACAAATAAGACAATGTGATACTTACTTAAGACTAGGGGAATTCAAAAATGGATACTTTGAAATGTCAG
  ATGTGAATCAAAAAATTCCCTTTGACATACATTGCATGAGGATATGTGTTACCAAAACACAGAAAGAATG
  GCTGATAATTAGAAGCATCAGTATTTGGACTTCCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001162368
• Insert Size: 1437 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001162368.1, NP_001155840.1
• RefSeq Size: 3862 bp
• RefSeq ORF: 1437 bp
• Locus ID: 67569
• UniProt ID: Q9D306
• Cytogenetics: 10 D1
• Gene Summary: Glycosyltransferase that participates in the transfer of N-acetylglucosamine (GlcNAc) to the core mannose residues of N-linked glycans. Catalyzes the formation of the GlcNAcbeta1-4 branch on the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans. Essential for the production of tri- and tetra-antennary N-linked sugar chains. Does not catalyze the transfer of GlcNAc to the Manalpha1-6 arm to form GlcNAcBeta1-4Manalpha1-6 linkage ('GnT-VI' activity) (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) differs in the 5' UTR compared to variant 4. All three variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.