Eif2s3x (NM_012010) Mouse Untagged Clone

CAT#: MC216487

Eif2s3x (untagged) - Mouse eukaryotic translation initiation factor 2, subunit 3, structural gene X-linked (Eif2s3x), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Eif2s3x
• Synonyms: AA409828; AA547477; AI314668; Eif-2gx
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216487 representing NM_012010
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGGGGGTGAGGGTGGAGTGACTCTCGGTCAGCCCCATCTTTCCCGACAGGATCTCGCCACATTGG
  ATGTTACCAAGTTGACACCACTTTCACATGAAGTTATCAGCAGACAAGCTACAATTAATATTGGTACAAT
  TGGTCACGTAGCTCATGGGAAGTCCACTGTGGTGAAGGCTATTTCTGGAGTTCACACTGTCAGATTCAAA
  AATGAACTCGAAAGGAACATTACCATCAAGCTTGGATATGCTAATGCTAAGATTTATAAACTTGATGACC
  CAAGTTGTCCTAGACCAGAATGTTACAGATCGTGTGGAAGCAGCACACCAGATGAGTTTCCTACAGACAT
  TCCGGGGACCAAAGGGAACTTCAAGTTAGTCAGGCATGTTTCCTTTGTTGACTGTCCTGGCCATGATATT
  TTGATGGCTACGATGCTGAACGGTGCAGCAGTGATGGATGCGGCCCTTCTGTTGATAGCTGGTAACGAAT
  CATGCCCTCAGCCCCAGACTTCCGAACACCTGGCTGCCATAGAAATCATGAAACTCAAGCATATTTTGAT
  CTTACAGAATAAAATTGATCTGGTAAAAGAAAGTCAGGCTAAGGAACAATATGAGCAAATCCTCGCATTT
  GTGCAAGGAACCGTAGCAGAAGGGGCACCAATTATTCCAATTTCTGCTCAACTGAAGTACAATATTGAAG
  TTGTCTGTGAGTATATAGTGAAGAAAATACCAGTGCCCCCAAGAGACTTTACTTCAGAGCCCCGTCTCAT
  TGTCATCAGGTCATTTGATGTCAACAAACCTGGCTGTGAAGTCGATGACCTTAAGGGAGGTGTAGCTGGT
  GGTAGTATCCTAAAAGGAGTGTTAAAGGTAGGCCAGGAGATAGAAGTGAGACCCGGTATTGTCTCCAAAG
  ACAGTGAAGGAAAACTCATGTGTAAACCAATCTTTTCCAAAATTGTGTCACTTTTTGCGGAACATAATGA
  TCTTCAATATGCTGCCCCGGGTGGTCTCATTGGAGTTGGAACAAAGATCGACCCCACCTTGTGCCGAGCT
  GACAGAATGGTGGGACAAGTGCTCGGTGCAGTTGGAGCTTTACCTGAAATATTCACCGAACTGGAGATTT
  CCTATTTCCTGCTTAGACGTCTTCTGGGTGTACGTACTGAAGGAGACAAGAAAGCAGCAAAGGTCCAAAA
  GCTGTCTAAGAATGAAGTGCTTATGGTCAACATAGGCTCCCTGTCGACAGGAGGAAGAGTTAGTGCAGTC
  AAGGCTGATTTGGGCAAAATTGTTTTGACCAATCCCGTGTGCACAGAAGTAGGAGAAAAAATTGCCCTTA
  GCAGAAGAGTTGAGAAACACTGGCGTTTAATTGGTTGGGGTCAGATAAGAAGAGGAGTGACCATTAAGCC
  AACTGTAGATGATGACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_012010
• Insert Size: 1419 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012010.3, NP_036140.1
• RefSeq Size: 3587 bp
• RefSeq ORF: 1419 bp
• Locus ID: 26905
• UniProt ID: Q9Z0N1
• Cytogenetics: X 41.52 cM
• Gene Summary: As a subunit of eukaryotic initiation factor 2 (eIF2), involved in the early steps of protein synthesis. In the presence of GTP, eIF2 forms a ternary complex with initiator tRNA Met-tRNAi and then recruits the 40S ribosomal complex, a step that determines the rate of protein translation. This step is followed by mRNA binding to form the 43S pre-initiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF2 and release of an eIF2-GDP binary complex. In order for eIF2 to recycle and catalyze another round of initiation, the GDP bound to eIF2 must exchange with GTP by way of a reaction catalyzed by eIF2B (By similarity). Along with its paralog on chromosome Y, may contribute to spermatogenesis up to the round spermatid stage (PubMed:26823431).[UniProtKB/Swiss-Prot Function]