Thrb (NM_009380) Mouse Untagged Clone

CAT#: MC216482

Thrb (untagged) - Mouse thyroid hormone receptor beta (Thrb), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Thrb
• Synonyms: c-erbAbeta; Nr1a2; T3Rbeta; T3R[b]; Thrb1; Thrb2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216482 representing NM_009380
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACTACTGTATGCCAGAGGTACACGAAGTGTGCCCAGCTGCCAGCAGCAACTGCTACATGCAGGTCA
  CTGACTACCTCGCATATCTGGAAGACAGTCCGGCTCTCAGTGGTCGTGATGTCCAAGCTGTGCCCAGTAG
  CAGCATATATATGGAACAGGCCTGGGCAGTGAATCAGCCTTATACCTGTAGTTACCCTGGAAACCTGTTT
  AAAAGCAAGGACTCTGACTTGGACATGGCCCTCAGTCAGTCCAGCCAGCCTGCACATCTCCCTGAAGAAA
  AGCCTTTTCCTCAAGTGCAGTCGCCACCGCACTCACAAAAAAAAGGGTATATCCCCAGCTATTTAGACAA
  AGATGAGCTCTGTGTAGTGTGTGGGGACAAAGCCACAGGGTACCACTATCGCTGCATCACCTGTGAAGGC
  TGCAAGGGCTTCTTTAGAAGAACCATTCAGAAAAGTCTCCATCCATCTTATTCCTGTAAATATGAAGGAA
  AGTGCATCATAGACAAAGTCACCCGCAACCAGTGCCAGGAATGTCGCTTTAAGAAATGCATCTATGTTGG
  CATGGCAACAGACTTGGTGCTGGATGACAGCAAGAGGCTAGCCAAGCGGAAGCTTATAGAGGAGAACCGT
  GAGAAGAGGCGGCGGGAAGAGCTACAGAAATCAATTGGGCACAAGCCAGAACCCACGGATGAGGAGTGGG
  AGCTCATCAAGACAGTCACTGAGGCCCACGTGGCCACCAATGCCCAGGGCAGCCACTGGAAGCAGAAGCG
  GAAATTCCTGCCAGAAGACATTGGACAAGCACCCATCGTGAATGCCCCAGAAGGTGGCAAGGTTGACCTG
  GAAGCCTTCAGTCATTTTACAAAAATCATCACACCAGCAATTACCAGAGTGGTGGATTTTGCCAAAAAGT
  TGCCCATGTTTTGTGAGCTGCCATGTGAAGACCAGATCATCCTCCTCAAAGGCTGCTGCATGGAGATCAT
  GTCCCTTCGAGCTGCTGTGCGCTATGACCCAGACAGCGAGACTCTAACTTTGAATGGGGAAATGGCAGTG
  ACACGAGGCCAGCTGAAAAATGGGGGCCTTGGGGTGGTTTCAGATGCCATCTTTGACCTGGGCATGTCTC
  TGTCATCTTTCAACCTGGATGACACTGAAGTCGCCCTGCTTCAGGCCGTCCTGCTAATGTCTTCAGATCG
  CCCAGGGCTAGCCTGTGTTGAGAGAATAGAGAAATACCAAGACAGTTTCCTGTTGGCCTTTGAACACTAT
  ATCAATTACCGGAAGCACCATGTGACACACTTTTGGCCCAAACTCCTGATGAAGGTGACAGACCTGCGGA
  TGATTGGAGCTTGCCATGCCAGCCGCTTCCTCCACATGAAGGTGGAGTGCCCCACTGAGCTCTTCCCGCC
  TCTGTTCTTAGAAGTATTTGAGGACTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009380
• Insert Size: 1428 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009380.3, NP_033406.1
• RefSeq Size: 6083 bp
• RefSeq ORF: 1428 bp
• Locus ID: 21834
• UniProt ID: P37242
• Cytogenetics: 14 A2
• Gene Summary: Nuclear hormone receptor that can act as a repressor or activator of transcription. High affinity receptor for thyroid hormones, including triiodothyronine and thyroxine.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) encodes the longer isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.