Mmp13 (NM_008607) Mouse Untagged Clone

CAT#: MC216470

Mmp13 (untagged) - Mouse matrix metallopeptidase 13 (Mmp13), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mmp13
• Synonyms: Cl; Clg; Mmp; MMP-1; MMP-13; Mmp1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216470 representing NM_008607
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATTCAGCTATCCTGGCCACCTTCTTCTTGTTGAGCTGGACTCCCTGTTGGTCCCTGCCCCTTCCCT
  ATGGTGATGATGATGATGATGACCTGTCTGAGGAAGACCTTGTGTTTGCAGAGCACTACTTGAAATCATA
  CTACCATCCTGCGACTCTTGCGGGAATCCTGAAGAAGTCTACAGTGACCTCCACAGTTGACAGGCTCCGA
  GAAATGCAATCTTTCTTTGGCTTAGAGGTGACTGGCAAACTTGATGATCCCACCTTAGACATCATGAGAA
  AACCAAGATGTGGAGTGCCTGATGTGGGTGAATACAATGTTTTCCCTAGAACACTCAAATGGTCCCAAAC
  GAACTTAACTTACAGGATTGTGAACTATACTCCTGATATGTCCCATTCTGAAGTGGAGAAGGCCTTCAGA
  AAAGCCTTCAAGGTCTGGTCTGATGTGACACCACTGAATTTCACCAGAATCTATGATGGCACTGCTGACA
  TCATGATATCTTTTGGGACTAAAGAACATGGTGACTTCTACCCATTTGATGGACCTTCTGGTCTTCTGGC
  ACACGCTTTTCCTCCTGGACCAAACTATGGTGGGGATGCCCATTTTGATGATGATGAAACCTGGACAAGC
  AGTTCCAAAGGCTACAACTTGTTTATTGTTGCTGCCCATGAGCTTGGCCACTCCCTAGGTCTGGATCACT
  CCAAGGACCCAGGAGCCCTGATGTTTCCCATCTATACCTACACTGGCAAAAGCCATTTCATGCTTCCTGA
  TGATGACGTTCAAGGAATTCAGTTTCTTTATGGTCCAGGCGATGAAGACCCCAACCCTAAGCATCCCAAA
  ACACCAGAGAAGTGTGACCCAGCCCTATCCCTTGATGCCATTACCAGTCTCCGAGGAGAAACTATGATCT
  TTAAAGACAGATTCTTCTGGCGCCTGCACCCTCAGCAGGTTGAGGCTGAGCTCTTTTTGACAAAGTCCTT
  TTGGCCAGAACTTCCCAACCATGTGGATGCTGCATATGAACATCCATCCCGTGACCTTATGTTTATCTTT
  AGAGGGAGAAAATTCTGGGCTCTGAATGGTTATGACATTCTGGAAGGTTATCCCAGAAAAATATCTGACC
  TGGGATTCCCAAAAGAGGTGAAGAGACTGAGCGCTGCGGTTCACTTTGAGAACACGGGGAAGACCCTCTT
  CTTCTCTGAGAACCACGTGTGGAGTTATGATGATGTTAACCAGACTATGGACAAAGATTATCCCCGCCTC
  ATAGAAGAGGAATTCCCTGGAATTGGCAACAAAGTAGATGCTGTCTATGAGAAAAATGGCTATATCTACT
  TTTTCAATGGGCCCATACAGTTTGAATACAGTATCTGGAGTAATCGCATTGTGAGAGTCATGCCAACAAA
  TTCCATATTGTGGTGTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008607
• Insert Size: 1419 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008607.2, NP_032633.1
• RefSeq Size: 2675 bp
• RefSeq ORF: 1419 bp
• Locus ID: 17386
• UniProt ID: P33435
• Cytogenetics: 9 A1
• Gene Summary: This gene encodes a member of the matrix metalloproteinase family that plays a role in wound healing, skeletal development and bone remodeling. The encoded protein is activated by the removal of an N-terminal activation peptide to generate a zinc-dependent endopeptidase enzyme that can cleave various native collagens, including types I - IV, X and XIV. Mice lacking the encoded protein display profound defects in growth plate cartilage as well as a delay in the endochondral bone development. Lack of the encoded protein also impairs the wound healing process due to reduced keratinocyte migration and vascular density at the wound site. This gene is located in a cluster of other matrix metalloproteinase genes on chromosome 9. [provided by RefSeq, Jun 2015]