Mmp10 (NM_019471) Mouse Untagged Clone

CAT#: MC216469

Mmp10 (untagged) - Mouse matrix metallopeptidase 10 (Mmp10), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mmp10
• Synonyms: AV377895; MMP-10; SL-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_019471, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGCCACTAGCCATCCTGGCACTGCTGAGCCTACCAATCTGCTCAGCGTATCCTCTGCATGGGGCAG
  TGACACAAGGCCACCCAAGCATGGATCTTGCTCAGCAATACCTAGAAAAATACTACAACTTTAAAAAAAA
  TGAGAAACAAATTTTCAAAAGAAAGGACAGTAGTCCTGTTGTCAAAAAAATTCAAGAAATGCAGAAGTTC
  CTCGGGTTGGAGATGACAGGGAAGCTGGACTCCAACACTATGGAGCTGATGCACAAGCCCAGGTGTGGTG
  TTCCTGATGTTGGTGGCTTCAGTACCTTCCCAGGTTCGCCAAAATGGAGGAAATCCCACATCACCTACAG
  GATTGTGAATTATACACCAGATTTGCCAAGACAGAGTGTGGATTCTGCCATTGAGAAAGCTTTGAAGGTC
  TGGGAGGAGGTGACCCCACTCACTTTCTCCAGGATCTCTGAAGGAGAGGCTGACATAATGATCTCCTTTG
  CAGTTGGAGAACACGGAGACTTTTACCCTTTTGATGGGCCAGGACAGAGTCTGGCTCATGCCTACCCACC
  TGGCCCTGGATTTTATGGAGATGTTCACTTCGATGATGATGAGAAATGGACACTTGCACCCTCAGGGACC
  AACTTATTCCTGGTTGCAGCCCATGAACTTGGCCACTCCCTGGGTCTCTTTCATTCCGACAAGAAAGAAT
  CTCTGATGTACCCAGTCTACAGGTTCTCCACAAGCCCAGCTAACTTCCACCTTTCTCAAGATGATATAGA
  GGGCATTCAATCCCTGTATGGAGCCGGTCCCTCCTCCGATGCCACAGTGGTTCCTGTGTTGTCTGTCTCT
  CCAAGACCTGAGACCCCAGACAAATGTGATCCTGCTTTGTCCTTTGATTCAGTCAGCACGCTGAGAGGGG
  AAGTCCTATTCTTTAAAGACAGGTACTTCTGGCGCAGATCCCATTGGAATCCCGAGCCTGAATTTCATTT
  GATATCAGCATTTTGGCCCACTCTTCCTTCAGACTTAGATGCTGCCTATGAGGCTCACAACACGGACAGT
  GTTCTGATTTTTAAAGGAAGTCAGTTCTGGGCAGTCCGAGGAAATGAAGTCCAAGCAGGCTACCCAAAGG
  GGATCCACACTCTCGGTTTTCCTCCCACCGTGAAGAAGATTGATGCAGCTGTTTTTGAAAAGGAGAAGAA
  GAAAACGTACTTCTTTGTAGGGGACAAATACTGGAGATTTGATGAGACAAGACATGTTATGGATAAAGGC
  TTCCCAAGACAGATAACAGATGATTTTCCAGGAATTGAGCCACAAGTTGATGCTGTGTTACACGAATTTG
  GGTTTTTTTATTTCTTCCGAGGATCATCACAGTTCGAGTTTGACCCCAATGCCAGGACGGTGACACACAT
  ACTGAAGAGCAACAGCTGGCTGCTGTGCTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_019471
• Insert Size: 1431 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC130027, AAI30028
• RefSeq Size: 1582 bp
• RefSeq ORF: 1431 bp
• Locus ID: 17384
• UniProt ID: O55123
• Cytogenetics: 9 A1
• Gene Summary: This gene encodes a member of the matrix metalloproteinase family of extracellular matrix-degrading enzymes that are involved in tissue remodeling, wound repair, progression of atherosclerosis and tumor invasion. The encoded preproprotein undergoes proteolytic processing to generate a mature, zinc-dependent endopeptidase enzyme. The lack of encoded protein in mice promotes experimental lung cancer formation, exacerbates experimental colitis and promotes development of inflammation-associated colonic dysplasia. This gene is located in a cluster of other matrix metalloproteinase genes on chromosome 9. [provided by RefSeq, Feb 2016]