Hyal2 (NM_010489) Mouse Untagged Clone

CAT#: MC216462

Hyal2 (untagged) - Mouse hyaluronoglucosaminidase 2 (Hyal2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Hyal2
• Synonyms: AI256841; AU020858; AW555733
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216462 representing NM_010489
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGGCAGGACTAGGTCCCATCATCACACTGGCCCTAGTGCTGGAGGTAGCATGGGCCGGGGAGCTTA
  AGCCCACAGCGCCGCCCATCTTCACTGGCCGACCCTTTGTGGTAGCATGGAACGTGCCCACACAAGAATG
  TGCCCCACGCCACAAAGTGCCACTGGACCTTAGGGCCTTCGATGTGAAGGCTACACCGAATGAGGGTTTT
  TTCAACCAGAATATCACCACCTTCTACTACGACCGTCTAGGCCTGTATCCACGTTTTGATGCAGCCGGGA
  CATCTGTGCATGGCGGTGTGCCTCAGAACGGCAGCCTCTGTGCACACCTGCCCATGCTGAAGGAATCTGT
  GGAACGCTACATCCAGACCCAGGAGCCTGGGGGGCTGGCAGTCATTGACTGGGAGGAATGGCGGCCTGTA
  TGGGTTCGAAACTGGCAGGAGAAAGATGTTTACCGACAGTCTTCACGCCAGCTGGTGGCCAGTCGGCACC
  CTGACTGGCCATCAGACAGAGTAATGAAGCAGGCCCAGTACGAGTTTGAGTTCGCCGCTCGGCAGTTCAT
  GTTGAACACTCTCCGTTACGTCAAGGCAGTCAGACCCCAGCACCTGTGGGGCTTCTACCTCTTTCCTGAC
  TGCTACAATCACGATTATGTACAGAACTGGGAGAGCTACACGGGCCGCTGTCCCGATGTGGAGGTGGCAC
  GGAACGACCAGCTGGCCTGGCTCTGGGCTGAGAGCACGGCTCTCTTTCCCTCTGTGTACCTGGACGAGAC
  ACTGGCGTCCTCCGTACACAGCCGCAACTTTGTCAGTTTCCGTGTTCGGGAGGCCCTTCGAGTGGCTCAC
  ACCCACCATGCCAACCACGCCCTCCCCGTGTACGTCTTCACGCGTCCCACATACACCCGAGGACTCACGG
  GACTGAGCCAGGTGGACCTTATCTCTACCATCGGTGAGAGTGCCGCCCTGGGCTCAGCTGGCGTCATCTT
  CTGGGGCGACTCGGAAGACGCTTCAAGTATGGAGACCTGCCAATACCTCAAGAATTACCTAACTCAGCTG
  CTGGTTCCCTACATAGTCAACGTGTCCTGGGCCACCCAGTATTGCAGTTGGACCCAGTGCCATGGCCATG
  GGCGATGTGTGCGCCGCAACCCCAGCGCCAATACCTTCCTGCACCTCAATGCCAGCAGCTTCCGCCTAGT
  GCCTGGCCATACCCCCAGTGAACCCCAGCTTCGACCCGAGGGGCAGCTCAGCGAAGCCGACCTCAACTAC
  CTGCAGAAGCACTTTCGCTGCCAGTGCTATCTGGGCTGGGGTGGCGAGCAGTGCCAACGGAACTATAAGG
  GGGCAGCTGGAAATGCCAGCAGAGCCTGGGCTGGATCCCACCTCACCAGCCTGCTGGGTTTGGTAGCTGT
  GGCTCTCACCTGGACCTTATGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_010489
• Insert Size: 1422 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010489.2, NP_034619.2
• RefSeq Size: 1872 bp
• RefSeq ORF: 1422 bp
• Locus ID: 15587
• UniProt ID: O35632
• Cytogenetics: 9 58.12 cM
• Gene Summary: Hydrolyzes high molecular weight hyaluronic acid to produce an intermediate-sized product which is further hydrolyzed by sperm hyaluronidase to give small oligosaccharides. Displays very low levels of activity. Associates with and negatively regulates MST1R (By similarity).[UniProtKB/Swiss-Prot Function]