Hnf4a (NM_008261) Mouse Untagged Clone

CAT#: MC216460

Hnf4a (untagged) - Mouse hepatic nuclear factor 4, alpha (Hnf4a), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Hnf4a
• Synonyms: Hnf; HNF-; HNF-4; Hnf4; Hnf4alpha; MODY; MODY1; Nr; Nr2a1; TCF-14; Tcf14
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216460 representing NM_008261
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGACTCTCTAAAACCCTTGCCGGCATGGATATGGCCGACTACAGCGCTGCCCTGGACCCAGCCTACA
  CCACCCTGGAGTTTGAAAATGTGCAGGTGTTGACCATGGGCAATGACACGTCCCCATCTGAAGGTGCCAA
  CCTCAATTCATCCAACAGCCTGGGCGTCAGTGCCCTGTGCGCCATCTGTGGCGACCGGGCCACCGGCAAA
  CACTACGGAGCCTCGAGCTGTGACGGCTGCAAGGGGTTCTTCAGGAGGAGCGTGAGGAAGAACCACATGT
  ACTCCTGCAGGTTTAGCCGACAATGTGTGGTAGACAAAGATAAGAGGAACCAGTGTCGTTACTGCAGGCT
  TAAGAAGTGCTTCCGGGCTGGCATGAAGAAGGAAGCTGTCCAAAATGAGCGGGACCGGATCAGCACGCGG
  AGGTCAAGCTACGAGGACAGCAGCCTGCCCTCCATCAACGCGCTCCTGCAGGCAGAGGTTCTGTCCCAGC
  AGATCACCTCTCCCATCTCTGGGATCAATGGCGACATTCGGGCAAAGAAGATTGCCAACATCACAGACGT
  GTGTGAGTCTATGAAGGAGCAGCTGCTGGTCCTGGTCGAGTGGGCCAAGTACATCCCGGCCTTCTGCGAA
  CTCCTTCTGGATGACCAGGTGGCGCTGCTCAGGGCCCACGCCGGTGAGCATCTGCTGCTTGGAGCCACCA
  AGAGGTCCATGGTGTTTAAGGACGTGCTGCTCCTAGGCAATGACTACATCGTCCCTCGGCACTGTCCAGA
  GCTAGCGGAGATGAGCCGTGTGTCCATCCGCATCCTCGATGAGCTGGTCCTGCCCTTCCAAGAGCTGCAG
  ATTGATGACAATGAATATGCCTGCCTCAAAGCCATCATCTTCTTTGATCCAGATGCCAAGGGGCTGAGTG
  ACCCGGGCAAGATCAAGCGGCTGCGGTCACAGGTGCAAGTGAGCCTGGAGGATTACATCAACGACCGGCA
  GTACGACTCTCGGGGCCGCTTTGGAGAGCTGCTGCTGCTGTTGCCCACGCTGCAGAGCATCACCTGGCAG
  ATGATCGAACAGATCCAGTTCATCAAGCTCTTCGGCATGGCCAAGATTGACAACCTGCTGCAGGAGATGC
  TTCTCGGAGGGTCTGCCAGTGATGCACCCCACACCCACCACCCCCTGCACCCTCACCTGATGCAAGAACA
  CATGGGCACCAATGTCATTGTTGCTAACACGATGCCCTCTCACCTCAGCAATGGACAGATGTGTGAGTGG
  CCCCGACCCAGGGGGCAGGCAGCCACTCCCGAGACTCCACAGCCATCACCACCAAGTGGCTCGGGATCTG
  AATCCTACAAGCTCCTGCCAGGAGCCATCACCACCATCGTCAAGCCTCCCTCTGCCATTCCCCAGCCAAC
  GATCACCAAGCAAGAAGCCATCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008261
• Insert Size: 1425 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC039220, AAH39220
• RefSeq Size: 4371 bp
• RefSeq ORF: 1425 bp
• Locus ID: 15378
• UniProt ID: P49698
• Cytogenetics: 2 84.32 cM
• Gene Summary: The protein encoded by this gene is a transcription factor involved in the development of the pancreas, liver, kidney, and intestines. The encoded protein also functions to maintain glucose homeostasis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2015]
  Transcript Variant: This variant (1) encodes the longest isoform (HNF4alpha2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.