Gabrg2 (NM_008073) Mouse Untagged Clone

CAT#: MC216456

Gabrg2 (untagged) - Mouse gamma-aminobutyric acid (GABA) A receptor, subunit gamma 2 (Gabrg2), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gabrg2
• Synonyms: GABAA-R; Gabrg-2; gamma2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216456 representing NM_008073
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTTCGCCAAATACATGGAGCATTGGAAGCTCAGTCTACTCTCCTGTATTTTCACAGAAAATGACGC
  TGTGGATTCTGCTCCTGCTATCGCTCTACCCAGGCTTCACAAGCCAAAAGTCAGATGATGACTATGAAGA
  TTACGCTTCTAATAAAACATGGGTGTTGACTCCAAAAGTTCCAGAGGGTGATGTCACTGTCATCTTAAAC
  AACCTGCTGGAAGGGTATGACAACAAACTTCGACCTGACATCGGAGTGAAACCAACATTAATTCATACAG
  ATATGTATGTGAACAGCATTGGTCCAGTGAATGCTATCAATATGGAATATACAATTGATATTTTTTTTGC
  CCAAACCTGGTATGACAGACGTTTGAAATTTAACAGTACCATTAAAGTTCTCCGGTTGAATAGCAATATG
  GTGGGGAAAATCTGGATTCCAGACACTTTCTTCAGGAACTCCAAAAAGGCTGATGCTCACTGGATCACCA
  CTCCCAACAGGATGCTGAGAATTTGGAATGATGGTCGAGTTCTCTACACCTTAAGGCTAACAATTGATGC
  TGAGTGCCAGTTGCAATTACACAACTTCCCAATGGATGAACACTCCTGCCCCCTGGAGTTCTCCAGTTAT
  GGATATCCTCGTGAAGAAATTGTTTATCAATGGAAGCGCAGTTCTGTTGAAGTGGGTGACACAAGATCAT
  GGAGGCTGTATCAATTTTCCTTTGTTGGATTGAGGAATACAACTGAAGTAGTGAAGACAACTTCTGGTGA
  CTATGTGGTGATGTCTGTGTACTTCGATCTGAGCAGAAGAATGGGCTACTTCACCATCCAGACTTACATT
  CCCTGCACACTCATCGTGGTCCTGTCCTGGGTGTCCTTCTGGATCAATAAGGATGCTGTTCCTGCCAGAA
  CATCTTTAGGAATCACTACTGTCCTGACCATGACAACTTTAAGCACCATAGCCAGAAAATCTCTGCCCAA
  GGTCTCCTATGTCACAGCAATGGATCTCTTTGTATCTGTTTGCTTCATCTTTGTGTTTTCTGCTTTGGTG
  GAGTATGGCACCCTGCATTATTTTGTCAGCAACCGGAAGCCAAGCAAGGATAAAGACAAAAAGAAGAAAA
  ACCCTCTTCTTCGGATGTTTTCCTTCAAGGCCCCTACCATTGATATTCGTCCCAGATCAGCAACCATTCA
  AATGAACAATGCCACACACCTTCAAGAGAGGGATGAAGAATATGGCTATGAGTGTTTGGATGGCAAGGAC
  TGTGCCAGTTTCTTCTGCTGTTTTGAAGATTGCCGAACAGGAGCCTGGAGACATGGGAGGATACATATTC
  GCATTGCCAAAATGGACTCCTATGCTCGGATCTTCTTCCCTACCGCCTTTTGCTTGTTCAATCTTGTTTA
  CTGGGTCTCCTATCTTTATCTGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008073
• Insert Size: 1425 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC031762, AAH31762
• RefSeq Size: 2304 bp
• RefSeq ORF: 1425 bp
• Locus ID: 14406
• UniProt ID: P22723
• Cytogenetics: 11 24.8 cM
• Gene Summary: This gene encodes a gamma-aminobutyric acid (GABA)-A receptor subunit, which is a member of the ligand-gated ion channel family. GABA is the major inhibitory neurotransmitter in the adult central nervous system, and conversely exhibits an excitatory function during development. GABA-A receptors are pentameric, consisting of proteins from several subunit classes: alpha, beta, gamma, delta and rho. This gene encodes one of three gamma subunits in mammals, which contain the binding site for benzodiazepine drugs. Several mutations in this gene are associated with epileptic seizures, and genetic knockdown is associated with anxiety behavior. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1, also known as gamma 2L). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data.