Gabrb1 (NM_008069) Mouse Untagged Clone

CAT#: MC216452

Gabrb1 (untagged) - Mouse gamma-aminobutyric acid (GABA) A receptor, subunit beta 1 (Gabrb1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gabrb1
• Synonyms: AW061132; B230208N19Rik; Gabrb-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216452 representing NM_008069
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGACAGTACAAAATCGAGAGAGTTTGGGGCTTCTCTCTTTTCCCGTGATGGTTGCTATGGTTTGTT
  GTGCACACAGCTCCAATGAACCCAGCAACATGTCATACGTGAAAGAGACAGTGGACCGACTGCTCAAAGG
  ATATGACATTCGCTTGCGGCCGGACTTTGGAGGGCCCCCGGTGGACGTCGGGATGCGGATCGATGTCGCC
  AGCATAGACATGGTCTCGGAAGTGAATATGGATTATACACTCACCATGTATTTCCAGCAGTCTTGGAAGG
  ACAAGAGGCTTTCATATTCTGGAATCCCATTAAATCTGACCCTTGACAACAGAGTAGCTGACCAACTCTG
  GGTACCAGACACCTACTTTCTCAATGACAAGAAATCATTTGTGCATGGGGTCACAGTGAAAAACCGAATG
  ATCAGGCTGCATCCTGATGGAACTGTTCTCTATGGACTACGGATCACAACCACTGCAGCATGCATGATGG
  ATCTTCGAAGATACCCTCTGGATGAGCAAAACTGCACCCTGGAGATTGAAAGTTATGGCTATACCACGGA
  TGACATCGAATTTTACTGGAATGGAGGAGAGGGAGCAGTAACTGGGGTCAATAAAATTGAGCTTCCCCAA
  TTTTCAATTGTTGACTACAAGATGGTGTCCAAGAAGGTGGAGTTCACAACAGGGGCATATCCACGACTAT
  CACTAAGTTTTCGTCTAAAGAGAAACATTGGTTACTTCATTTTGCAGACCTACATGCCTTCCACACTGAT
  TACAATCCTGTCCTGGGTGTCGTTTTGGATCAACTATGATGCATCTGCAGCCAGAGTCGCACTAGGAATC
  ACTACGGTGCTGACCATGACCACCATCAGCACTCACCTCAGGGAGACTTTGCCGAAGATCCCTTACGTCA
  AAGCGATTGACATCTATCTCATGGGCTGTTTTGTGTTTGTGTTCCTGGCTCTACTGGAATATGCTTTTGT
  CAATTACATTTTCTTCGGAAAAGGCCCTCAGAAAAAGGGAGCGAGCAAACAAGACCAGAGTGCCAATGAA
  AAGAACAGACTGGAGATGAACAAAGTCCAAGTTGATGCCCACGGCAATATTCTCCTCAGCACCCTGGAAA
  TCAGGAACGAGACCAGTGGCTCCGAGGTGCTCACAGGTGTAAGTGACCCCAAGGCCACCATGTACTCGTA
  CGACAGCGCCAGCATCCAGTACCGCAAGCCACTGAGCAGCCGCGAGGGCTTCGGGCGTGGGCTGGACAGG
  CACGGGGTACCGGGCAAGGGGCGCATCCGCAGGCGCGCCTCGCAGCTCAAAGTGAAGATCCCCGACTTGA
  CTGACGTGAACTCCATAGACAAGTGGTCCCGAATGTTCTTCCCCATCACCTTTTCTCTTTTTAATGTCGT
  TTATTGGCTTTACTATGTACACTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_008069
• Insert Size: 1425 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008069.4, NP_032095.1
• RefSeq Size: 1860 bp
• RefSeq ORF: 1425 bp
• Locus ID: 14400
• UniProt ID: P50571
• Cytogenetics: 5 38.18 cM
• Gene Summary: Component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the vertebrate brain. Functions also as histamine receptor and mediates cellular responses to histamine (By similarity). Functions as receptor for diazepines and various anesthetics, such as pentobarbital; these are bound at a separate allosteric effector binding site. Functions as ligand-gated chloride channel.[UniProtKB/Swiss-Prot Function]