Smyd1 (NM_009762) Mouse Untagged Clone

CAT#: MC216431

Smyd1 (untagged) - Mouse SET and MYND domain containing 1 (Smyd1), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Smyd1
• Synonyms: 4632404M21Rik; Bop; C78565; Zmynd18
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216431 representing NM_009762
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAATAGGCAGCATGGAGAACGTGGAGGTCTTCACTTCCGAGGGCAAAGGCAGAGGTCTGAAGGCTA
  CGAAGGAGTTCTGGGCTGCGGATGTCATCTTTGCGGAGAGGGCTTATTCTGCAGTGGTTTTTGACAGCCT
  CATTAACTTCGTGTGCCACACCTGCTTCAAGAGGCAGGAGAAGCTCCATCGCTGCGGGCAGTGCAAGTTC
  GCCCATTACTGCGACCGCACGTGCCAGAAGGATGCTTGGCTGAATCACAAGAACGAGTGCGCTGCCATCA
  AGAAATATGGGAAAGTGCCCAACGAGAACATCAGGCTGGCCGCCCGCATCATGTGGCGGGTGGAGAGAGA
  GGGCACTGGGCTCACAGAGGGCTGCCTGGTGTCCGTGGATGACTTACAGAACCACGTGGAGCACTTTGGG
  GAGGAGGAGCAGAAGGAACTCCGAGTAGACGTGGACACGTTCTTGCAGTACTGGCCACCACAGAGCCAGC
  AGTTCAGCATGCAGTATATCTCACACATCTTTGGTGTGATCAACTGCAACGGTTTCACTCTCAGTGACCA
  GAGAGGGCTACAGGCAGTAGGTGTGGGCATCTTCCCCAACCTGGGCCTGGTGAACCATGATTGCTGGCCA
  AACTGCACTGTCATATTCAACAATGGCAAGATTGAGCTCCGGGCCCTGGGCAAGATCTCAGAAGGCGAGG
  AGCTGACTGTGTCCTACATAGACTTCCTGCACCTCAGTGAGGAGCGCAGGCGGCAGCTGAAGAAACAGTA
  CTACTTTGACTGCTCCTGTGAGCACTGCCAGAAGGGGCTGAAGGACGACCTCTTCCTGGCAGCGAAGGAA
  GACCCCAAGCCCTCCCAGGAAGTGGTGAAGGAGATGATACAATTCTCAAAGGATACACTGGAAAAAATAG
  ACAAGGCTCGCTCCGAGGGTTTGTATCACGAGGTTGTGAAGCTGTGTCGGGAGTGCCTGGAGAAGCAGGA
  GCCAGTGTTCGCCGACACCAACCTCTACGTGCTTCGGCTGCTCAGCATTGCATCAGAGGTCCTCTCCTAC
  CTCCAGGCCTATGAGGAGGCCTCACATTATGCCAGGAGGATGGTTGATGGCTACATGAAACTCTACCACC
  ATAACAATGCCCAACTGGGCATGGCTGTGATGAGGGCAGGGCTTACCAACTGGCATGCTGGTCACATCGA
  AGTGGGGCATGGGATGATCTGCAAAGCCTATGCTATTCTCCTGGTGACCCATGGACCCTCCCACCCTATC
  ACCAAAGACTTAGAGGCCATGCGGATGCAGACAGAGATGGAGCTGCGTATGTTCCGCCAAAACGAATTCA
  TGTATCACAAGATGCGAGAGGCTGCCCTGAACAACCAGCCCATGCAGGTCATGGCTGAGCCTAGCAATGA
  ACCAGCCCCCGCTCTGTTCCATAAGAAGCAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009762
• Insert Size: 1434 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009762.2, NP_033892.2
• RefSeq Size: 3340 bp
• RefSeq ORF: 1434 bp
• Locus ID: 12180
• UniProt ID: P97443
• Cytogenetics: 6 32.14 cM
• Gene Summary: Methylates histone H3 at 'Lys-4' (H3K4me). Acts as a transcriptional repressor. Essential for cardiomyocyte differentiation and cardiac morphogenesis.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an in-frame exon in the CDS, as compared to variant 1. The resulting isoform (2) lacks an internal segment, as compared to isoform 1. CCDS Note: The coding region has been updated to extend the N-terminus to one that is supported by available conservation data.