Vipas39 (NM_001142581) Mouse Untagged Clone

CAT#: MC216423

Vipas39 (untagged) - Mouse VPS33B interacting protein, apical-basolateral polarity regulator (Vipar), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Vipas39
• Synonyms: 6720456H09Rik; 9330175H22Rik; AI413782; hSPE-39; SPE-39; Spe39; Vipar
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216423 representing NM_001142581
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCGGACAAAAGGTGATGAGGAGGAATATTGGAACAGCTCCAAGTTCAAGGCTTTCACTTTTGATG
  ATGAAGATGATGAGCTCTCACAGTTAAAGGAATCCAAACGCGCAGTGAATAGCCTTCGAGACATCGTGGA
  TGACGACGATGACGATGACCTGGAGAGAGTCAGCTGGACTGGAGAACCTGTGGGAAGTATCTCATGGTCC
  ATCAAAGAGACTGCTGGGAGTAGCGGGTCAACCCCTGAGGGACGTGAACAGCTAAAGGGCCGAAACAGCT
  TCTACACACAGCTACCCAAACCTCCTTCTACTTACTCCCTCAGCAGCTTCTTCAGAGGTAGAACTAGACC
  TGGGAGTTTCCAGTCCCTTTCTGATGCTCTGTCAGACACACCTGCCAAAAGTTATGCTCCAGAGCTGGGG
  AGGCCTAAGGGAGAATACAGGGTCTGTTCACTAGAGCGCTTCCGCTCCCTCCAGGATAAGCTGCAGCTCC
  TAGAGGAGGCAGTAAGCATGCATGATGGGAACGTCATCACTGCAGTTCTGATCTTCCTGAAGAGGACCCT
  GAGCAAAGAGATCCTTTTTCGAGAGCTGGAGGTACGCCAGGTCGCACTGAGACATCTCATTCATTTTCTT
  AAAGAAATAGGGGACCAAAAGCTGCTTTTAGATCTCTTCAGGTTCCTAGATAGAGCAGAAGAACTGGCGC
  TATCCCATTATCGAGAGCACTTGAACATTCAGGACCCTGAAAAACGAAAAGAATTTCTTAAGACTTGCAT
  TGGTTTGCCATTTTCAGCAGAAGACGCTGCGCATGTACAAGACCATTACACCCTGCTGGAGCGCCAGATC
  ATCATCGAGGCAAACGACCGGCACCTGGAGTCTTCAGGGCAGACTGATATCTTCCGGAAGCACCCCCGTA
  AAGCTTCCATTCTCAACATGCCGTTAGTGACGACACTCTTCTACGCCTGCTTCTATCACTACACGGAGTC
  TGAGGGAACCTTCAGCAGTCCCATCAACCTGAAGAAAACATTCAAGATCCCAGACAAACAGTATGTGCTG
  ACAGCCTTGGCTGCGCGTGCCAAGCTGAGAGCCTGGAATGACGTTGATGCCCTGTTCACCACAAAGAACT
  GGTTGGGTTACACCAAGAAGAGAGCACCCATTGGCTTCCATCGAGTTGTGGAAATTTTGCACAAGAACAG
  TGCCCCTGTCCAGATACTGCAGGAATATGTCAATCTGGTGGAAGATGTGGACACCAAGTTGAACTTAGCT
  ACTAAGTTCAAGTGCCATGATGTCGTCATTGATACTTGCCGAGACCTGAAGGATCGTCAGCAGTTGCTTG
  CATACAGGAGCAAAGTAGACAAAGGATCCGCCGAGGAAGAGAAGATTGACGCCATTCTCAGCAGCTCGCA
  AATTCGATGGAAGAACTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001142581
• Insert Size: 1419 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001142581.1, NP_001136053.1
• RefSeq Size: 2495 bp
• RefSeq ORF: 1419 bp
• Locus ID: 104799
• UniProt ID: Q8BGQ1
• Cytogenetics: 12 D2
• Gene Summary: Proposed to be involved in endosomal maturation implicating in part VPS33B. In epithelial cells, the VPS33B:VIPAS39 complex may play a role in the apical RAB11A-dependent recycling pathway and in the maintenance of the apical-basolateral polarity (PubMed:20190753). May play a role in lysosomal trafficking, probably via association with the core HOPS complex in a discrete population of endosomes; the functions seems to be indepenedent of VPS33B (By similarity). May play a role in vesicular trafficking during spermatogenesis (By similarity). May be involved in direct or indirect transcriptional regulation of E-cadherin.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. It encodes isoform b, which is shorter than isoform a. Variants 2 and 3 both encode isoform b. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.