Sec61a1 (NM_016906) Mouse Untagged Clone

CAT#: MC216417

Sec61a1 (untagged) - Mouse Sec61 alpha 1 subunit (S. cerevisiae) (Sec61a1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Sec61a1
• Synonyms: AA408394; AA410007; Sec61a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216417 representing NM_016906
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGATCAAATTTCTGGAAGTCATCAAGCCATTCTGTGTCATCTTGCCGGAAATTCAGAAGCCAGAGA
  GAAAGATTCAGTTTAAGGAGAAAGTTCTGTGGACAGCCATCACCCTCTTCATCTTCTTAGTATGCTGTCA
  GATCCCCCTGTTTGGCATCATGTCTTCAGACTCAGCTGACCCGTTCTACTGGATGCGAGTGATTCTGGCC
  TCTAACAGAGGTACCTTAATGGAGCTGGGCATCTCTCCTATTGTCACCTCCGGTCTTATCATGCAGCTCT
  TAGCTGGTGCCAAGATAATTGAAGTTGGGGACACCCCGAAAGACCGAGCTCTCTTCAATGGAGCCCAGAA
  GTTGTTTGGAATGATCATTACTATCGGTCAGTCCATTGTGTATGTGATGACGGGCATGTACGGGGACCCT
  TCGGAGATGGGTGCTGGAATCTGCCTGCTGATCACCATTCAGCTCTTTGTTGCTGGCTTAATTGTCCTAC
  TTTTGGATGAACTTCTGCAAAAAGGGTACGGCTTGGGCTCTGGCATTTCTCTCTTCATTGCGACTAACAT
  CTGTGAGACCATTGTGTGGAAGGCATTCAGCCCCACCACTGTCAACACTGGCCGAGGGATGGAATTCGAA
  GGCGCCATCATAGCGCTATTCCACCTGCTGGCCACCCGCACAGACAAGGTCCGAGCCCTTCGAGAGGCAT
  TCTACCGCCAGAACCTCCCCAACCTCATGAACCTGATTGCCACCATCTTCGTCTTTGCCGTTGTCATCTA
  TTTCCAGGGCTTCCGAGTGGACCTTCCAATCAAGTCGGCGCGTTACCGAGGCCAGTACAACACCTATCCC
  ATCAAGCTCTTCTACACCTCCAACATCCCCATCATCTTGCAGTCTGCTCTGGTGTCCAACCTGTATGTCA
  TCTCCCAGATGCTGTCAGCACGCTTCAGTGGCAACCTGCTGGTTAGCCTGCTGGGCACCTGGTCGGATAC
  ATCATCTGGGGGCCCAGCCCGAGCTTACCCCGTTGGTGGCCTGTGTTACTACCTGTCTCCTCCGGAATCG
  TTCGGCTCAGTGCTGGAGGACCCTGTCCATGCGGTCGTGTACATCGTGTTCATGCTGGGCTCCTGTGCAT
  TCTTCTCCAAAACATGGATCGAAGTCTCAGGTTCTTCTGCCAAAGATGTTGCAAAGCAGCTGAAGGAGCA
  GCAGATGGTGATGAGGGGCCACAGGGAGACCTCCATGGTGCATGAGCTCAACCGGTACATCCCCACAGCC
  GCAGCCTTTGGTGGGCTGTGCATTGGGGCCCTCTCTGTCCTGGCTGACTTCCTGGGTGCTATCGGGTCTG
  GAACTGGGATCCTGCTTGCGGTCACCATCATCTACCAGTACTTTGAGATCTTCGTCAAGGAACAGAGCGA
  GGTGGGCAGCATGGGAGCCCTTCTCTTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_016906
• Insert Size: 1431 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016906.4, NP_058602.1
• RefSeq Size: 3039 bp
• RefSeq ORF: 1431 bp
• Locus ID: 53421
• UniProt ID: P61620
• Cytogenetics: 6 D1
• Gene Summary: Component of SEC61 channel-forming translocon complex that mediates transport of signal peptide-containing precursor polypeptides across endoplasmic reticulum (ER). Forms a ribosome receptor and a gated pore in the ER membrane, both functions required for cotranslational translocation of nascent polypeptides. May cooperate with auxiliary protein SEC62, SEC63 and HSPA5/BiP to enable post-translational transport of small presecretory proteins. Controls the passive efflux of calcium ions from the ER lumen to the cytosol through SEC61 channel, contributing to the maintenance of cellular calcium homeostasis (By similarity). Plays a critical role in nephrogenesis, specifically at pronephros stage (PubMed:27392076).[UniProtKB/Swiss-Prot Function]