Ctsa (NM_001038492) Mouse Untagged Clone

CAT#: MC216412

Ctsa (untagged) - Mouse cathepsin A (Ctsa), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ctsa
• Synonyms: AU019505; P; Pp; PPCA; Ppgb
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216412 representing NM_001038492
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCGGAACCGCGCTGTCTCCACTGCTCTTGTTGCTGCTCCTGTCCTGGGCGTCCCGGAACGAAGCAG
  CTCCGGACCAGGATGAAATCGATTGTCTCCCCGGCCTGGCCAAGCAGCCCTCTTTCCGGCAATACTCCGG
  CTACCTCAGAGCATCGGACTCCAAGCACTTCCACTACTGGTTTGTGGAGTCGCAGAACGACCCAAAGAAC
  AGCCCCGTGGTGCTTTGGCTTAACGGGGGTCCCGGCTGCAGCTCGCTCGATGGGCTGCTTACAGAGCACG
  GCCCCTTTCTGATCCAGCCAGATGGTGTCACCCTGGAGTACAACCCCTATGCTTGGAACCTGATTGCCAA
  CGTGCTGTATATCGAGTCCCCAGCTGGGGTGGGCTTCTCCTACTCGGATGACAAGATGTACGTGACCAAT
  GACACAGAGGTGGCGGAGAACAATTATGAAGCCCTTAAAGACTTCTTCCGCCTCTTTCCGGAATACAAGG
  ACAACAAACTTTTCCTGACAGGAGAGAGCTATGCTGGCATCTACATCCCCACCTTGGCTGTACTGGTCAT
  GCAGGATCCTAGCATGAATCTTCAGGGGCTGGCTGTGGGCAATGGACTTGCCTCCTATGAGCAGAACGAC
  AACTCCCTGGTCTACTTTGCCTACTACCATGGCCTTCTGGGGAACAGACTTTGGACTTCACTGCAGACCC
  ACTGCTGCGCTCAGAACAAGTGTAACTTCTATGACAACAAAGACCCAGAGTGTGTAAACAATCTCCTGGA
  AGTGTCTCGAATTGTGGGCAAATCTGGCCTCAACATCTACAATCTCTATGCTCCGTGTGCTGGTGGGGTG
  CCCGGCAGACATAGATATGAGGACACACTTGTAGTCCAGGATTTTGGCAACATCTTCACTCGCCTGCCAC
  TTAAGCGGAGATTTCCTGAGGCACTGATGCGTTCTGGGGACAAGGTACGCTTGGATCCTCCCTGCACCAA
  CACCACAGCCCCTTCCAACTACCTCAACAACCCCTATGTTCGGAAGGCTCTCCACATCCCCGAGTCGCTG
  CCCCGCTGGGACATGTGCAACTTCTTGGTGAATTTACAGTACCGCCGCCTCTACCAAAGCATGAACTCCC
  AGTACCTGAAGCTGCTCAGTTCACAGAAATACCAGATCCTGCTCTACAACGGAGATGTGGACATGGCCTG
  CAACTTCATGGGCGATGAGTGGTTTGTGGATTCGCTCAACCAGAAGATGGAGGTGCAGCGCCGGCCCTGG
  CTAGTGGACTACGGGGAGAGCGGAGAACAGGTAGCTGGTTTCGTGAAGGAGTGTTCACACATCACCTTCC
  TCACCATCAAGGGTGCCGGACACATGGTCCCCACGGACAAGCCTCGAGCTGCTTTTACCATGTTCTCGAG
  GTTCCTGAACAAAGAGCCTTACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001038492
• Insert Size: 1425 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001038492.2, NP_001033581.1
• RefSeq Size: 3548 bp
• RefSeq ORF: 1425 bp
• Locus ID: 19025
• UniProt ID: P16675
• Cytogenetics: 2 85.27 cM
• Gene Summary: This gene encodes a glycoprotein with deamidase, esterase and carboxypeptidase activities. The encoded protein associates with and provides a protective function to the lysosomal enzymes beta-galactosidase and neuraminidase. Deficiency of the related gene in humans results in galactosialidosis. The proprotein is processed into two shorter chains. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013]
  Transcript Variant: This variant (2) differs in the 5' UTR, and initiates translation at an alternate downstream in-frame start codon, compared to variant 1. The encoded isoform (b) has a shorter N-terminus, compared to isoform a.