Trim60 (NM_153097) Mouse Untagged Clone

CAT#: MC216371

Trim60 (untagged) - Mouse tripartite motif-containing 60 (Trim60), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Trim60
• Synonyms: 2czf45; Rnf33
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216371 representing NM_153097
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTCCACAGCCCTAAAAATCTTGCAGGACAAATGTATCTGTTACATTTGTTCGGATTTCATGGAAG
  ACCCAGTGACCAGTAGGTGTGGGCACAACTTCTGTTTTGCCTGCCTCCGTTTGCTCTGGGATGATCTACA
  AGGCAACATCTTCTGTCCCGTCTGTCAAACCCCCTTTCCACCGAAAAGCTTCAGCAGGAACTACCAGTTC
  CGTAACATGACTGAAACCATTAGGCTCCTGCAAAAGAGACAGAGCAAGAGGAAGAGGCAGGAAGAACACA
  CAGTATGTCCGAAGCATGATCAGCCTCTGGTCTTGTTCTGTGTGAGGGATCGGGATGTCTTATGTACCCA
  GTGTAGCCTCTCTGTCGAACACCAGGGCCATTACACCTGCCCCATTAAGAAAGCCAGCTCTTATCACAGG
  AAAGTCCTAGAGAGCGCCATTGCAACGTTAAAGTTCGGAGTGAAACAAGTTGAAGAAAAGCTAGCTGTGC
  AGCACAGAAGGGTTCTTGGACTGAGGGAGGAGGCTCAATATCAAAAAATAGAGATACGTTATGAAATTGG
  GCAAATCAAACTGTTCCTACAAAGTGAGTACGAAGCGCATCTAAATGAGAGCCACATGGAGGAGCTGCGG
  AGTTTCTCAGAACTGAATGGATATCTTGAGACCCTGTTAGACCATGTTTCCACAGCAAAAGATCTGTTGA
  AGGAAGTGGAGGCCATACATGAAAGGTCAGATGTCACACTCCTGAGAGCCTACCACAAGTTGCAAAACCT
  TAAATCCCCCAAACCGTGGTTATTTAGGACAAAGCAATATGGCCTCAGTCTCCCAGCGCAGTATTCTGGC
  TTGAGCAGAATCATCAAGCAATTTCAAGCAGATGTGACTTTTGATCGTGACACAGCACACCCTCAGCTGG
  TTATTTCTGAGGATAGAAAATCTGTGTTTTATAAAGAAGCATGGCCATGTGTGTGTGCTAGCCCTCAGAA
  ATTTCACCTCTGGCCCGCTCTCCTGGGATGTAAAGGATTCGATTCTGGTCGACAGTACTGGGAAGTCAAA
  GTGGGAGACAAGCCCAGATGGACACTGGGTGTGTGTCAAGCCCACTTTTCTGGGGACTGGAGCAATCAGT
  CCTCAGGTTTCTGGGCAATTGGGCGGTATGCTGAGAATAGTTATGTCACCTATGGACCTCTAAGAACGGA
  GTTTCTGCCAGTGGTGCGACCCAGCAAGGTCGGCATATTTTTGGATTATGAATTGGGTGAGCTCTCCTTT
  TATAATATGAATGACAGGTCTCTTCTCTACACTTTCAGGAATTCTTTTACCAGCACCCTTTGGCCTTATT
  TCTATATAGGAACAGATTCTGAATCTCTTGAAATCTTAACCCACCCCACCCCAGACACAGGGTCTTACTA
  A
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_153097
• Insert Size: 1401 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153097.2, NP_694737.1
• RefSeq Size: 3633 bp
• RefSeq ORF: 1401 bp
• Locus ID: 234329
• UniProt ID: Q8VI40
• Cytogenetics: 8 B3.1