Trim11 (NM_053168) Mouse Untagged Clone

CAT#: MC216348

Trim11 (untagged) - Mouse tripartite motif-containing 11 (Trim11), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Trim11
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216348 representing NM_053168
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGCCCCAGACTTGTCCACCAACCTCCAGGAGGAGGCCACCTGCGCCATCTGCCTGGATTACTTCA
  CCGACCCGGTGATGACCGACTGCGGCCACAACTTCTGCCGCGAGTGCATCCGACGTTGCTGGGGCCAGCC
  CGAGGGCCCATACGCGTGCCCCGAGTGTCGCGAGCTGTCGGCGCAGAGGAACCTACGGCCCAACCGCCCG
  CTCGCCAAAATGGCAGAAATGGCACGACGCCTGCACCCGCCTTCTCCGGTCCCGCAGGGCGTCTGTGCGG
  CGCACCGCGAGCCGCTGACCACCTTCTGCGGCGACGACCTCAGCCTGCTGTGCCCCATCTGCGAGCGTTC
  GGAGCACTGGACTCACCGGGTGAGACCGCTGCAGGAGGCGGCGGACGACCTCAAGGGGAGGCTGGAGAAG
  TCACTGGAGCACCTACGGAAACAAATGGAGGACGCAATGCTGTTCCAAGCCCAGGCTGAGGAAACCTGTG
  CCTTGTGGCAGAAGATGGTGGAGAGCCAGCGGCAGAATGTGCTGGGCGAGTTTGAGAGGCTGCGCCGCCT
  GTTGGCAGAGGAGGAGCAACAGCTGCTGCAGAAGCTGGAGGAGGAAGAGCTGGAGGTTCTGCCGCGCCTG
  CGTGAAGGCGCTGCGAGGCTTGGCCAGCAAAGCACGCAGCTGGCAGCCCTCATCTCCGAGCTTGAGAGCC
  GCTGCCAGCTGCCAGCCCTGGGTTTACTGCAGGACATTAAGGACGCCCTGTGCAGGGTGCAGGATGTGAA
  GCTGCAGCCTCCAGCAGTGGTGCCCATGGAGCTGAGGACCGTGTGCCGGGTCCCAGGGCTGGTGGAGACC
  CTGCGGAGGTTCCGAGGGGACATAACCCTGGACCCAGACACCGCCAACCCTGAGCTGGTCTTATCTGAGG
  ACCGGAGGAGTGTGCAGCGTGGTGAACAGCGGCAGGCCCTGCCTGACAACCCAGAGCGGTTCGACCCCGG
  CCCTTGCGTGCTGGGCCAGGAGCGCATTACCTCTGGCCGCCACTACTGGGAGGTGGAAGTCGGGGACCAG
  ACCAGCTGGGCACTCGGCGTGTGTAAAGAAACTGCCAACAGGAAGGAGAAGGGGGAGCTGTCGGCTGGCA
  ATGGGTTCTGGATCCTGGTGTTCCTGGGGAGTTTCTATAATTCCAATGAGCCGGCCTTCTCCCCACTGCG
  GGACCCTCCCAAGCGTGTGGGGATTTTTCTGGACTATGAAGCTGGCCATCTCTCATTCTACAGTGCCACG
  GATGGGTCGCTGCTGTTTATCTTCCCCGAGACCCTGTTCTCAGGGACACTCCGGCCCCTCTTCTCACCTC
  TGTCAAGCAGCCCGACCCCTATGACTATCTGCAGGCTGATAGGTGTATCTGGGGACACCCTTGGCCCGCA
  GTGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-RsrII
• ACCN: NM_053168
• Insert Size: 1404 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053168.2, NP_444398.1
• RefSeq Size: 2279 bp
• RefSeq ORF: 1404 bp
• Locus ID: 94091
• UniProt ID: Q99PQ2
• Cytogenetics: 11 B1.3
• Gene Summary: E3 ubiquitin-protein ligase that promotes the degradation of insoluble ubiquitinated proteins, including insoluble PAX6, poly-Gln repeat expanded HTT and poly-Ala repeat expanded ARX. Mediates PAX6 ubiquitination leading to proteasomal degradation, thereby modulating cortical neurogenesis. May also inhibit PAX6 transcriptional activity, possibly in part by preventing the binding of PAX6 to its consensus sequences. May contribute to the regulation of the intracellular level of HN (humanin) or HN-containing proteins through the proteasomal degradation pathway. Mediates MED15 ubiquitination leading to proteasomal degradation. May contribute to the innate restriction of retroviruses. Upon overexpression, reduces HIV-1 and murine leukemia virus infectivity, by suppressing viral gene expression. Antiviral activity depends on a functional E3 ubiquitin-protein ligase domain. May regulate TRIM5 turnover via the proteasome pathway, thus counteracting the TRIM5-mediated cross-species restriction of retroviral infection at early stages of the retroviral life cycle.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an in-frame exon, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1.