Mmp1a (NM_032006) Mouse Untagged Clone

CAT#: MC216346

Mmp1a (untagged) - Mouse matrix metallopeptidase 1a (interstitial collagenase) (Mmp1a), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mmp1a
• Synonyms: Mco; Mcol-A; Mcola; MMP-1a
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216346 representing NM_032006
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTAGCCTTCCTTTGCTGTTGCTTCTCTGGGCTGCTAGCTCATACAGTTTCCCTGTGTTTCACAACG
  GAGACCGGCAAAATGTGGAGACAGTCTGGAAATACCTGGAAAACTACTACAACTTGGGCAAAAACATGCA
  AGCTAAAAACGTGAATGGCAAGGAGATGATGGCTGAAAAGCTGAGGCAAATGCAGCAGTTATTTGGGCTG
  AAAGTGACTGGAAATTCAGATCCTGAAACCCTGAGAGCTATGAAGAAGCCCAGGTGTGGGGTGCCTGATG
  TGGCCCCATATGCCATTACTCACAACAATCCTCGTTGGACCAAAACACATCTGACATACAGCATTTTAAA
  CTACACACCATATTTGCCAAAAGCAGTTGTGGAAGATGCCATCGCGAGAGCCTTTAGAGTCTGGAGTGAT
  GTGACACCACTTACGTTCCAAAGAGTCTTTGAGGAGGAAGGCGATATTGTGCTCTCCTTCCACAGAGGAG
  ACCATGGTGACAACAACCCATTTGATGGACCTAACTATAAGCTTGCTCACACTTTCCAGCCAGGCCCAGG
  TTTGGGGGGTGATGTTCATTATGACCTTGATGAGACGTGGACCAACAGCAGTGAAAATTTCAACTTGTTC
  TATGTTACGGCTCATGAACTGGGTCACTCCCTTGGGCTCACTCATTCTAGTGATATAGGAGCACTAATGT
  TCCCCAGTTACACGTGGTACACTGAAGACTTTGTGCTAAACCAGGATGATATTAATCGCATCCAGGACTT
  ATATGGACCTTCCCCAAATCCCATCCAGCCAACAGGTGCAACAACACCACATCCATGTAATGGTGATCTA
  ACCTTTGATGCTATAACTACATTTAGGGGAGAGGTGTTTTTCTTCAAAGGCAGGTTCTACATTCGGGTAA
  ATAGATTCATGCCAGAACCTGAGCTCAATTTAATAGGTATTCTCTGGCCAAATCTTCCAGTTAAACTTGA
  CGCTGCTTATGAAGCTAGTATGATAGATCAAGTCCGCTATTTCAAAGGCAGCAAAGTATGGGCTGTTCAA
  GAGCAGAGTGTACTGAGAGGATTCCCCAGAGACATCCACAGTTTCTTTGGCTTCCCTAGCAATGTGACAC
  ACATTGATGCTGCTGTTTGTGAGGAAGAGACTGGAAAAACATACTTCTTTGTTGACCACATGTACTGGAG
  GTATGATGAAAATACACAGTCTATGGATCCAGGTTATCCCAGATTAACAGCAGAAGACTTCCCTGGAATT
  GATGATAAAGTTGATGATGTTTTCCAAAAAGGAGAAAATTTCTATTTCTTTCACCAATCAGTTCAACACA
  GATTTAACCTCCAAATAAGAAGAGTTGATGATTCCCGTGATTCTAGTACATGGTTCAACTGCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_032006
• Insert Size: 1395 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032006.3, NP_114395.1
• RefSeq Size: 2025 bp
• RefSeq ORF: 1395 bp
• Locus ID: 83995
• UniProt ID: Q9EPL5
• Cytogenetics: 9 A1
• Gene Summary: This gene encodes a member of the matrix metalloproteinase family of extracellular matrix-degrading enzymes that are involved in tissue remodeling, wound repair, progression of atherosclerosis and tumor invasion. The encoded preproprotein undergoes proteolytic processing to generate a mature, zinc-dependent endopeptidase enzyme that degrades collagens. Mice lacking the encoded protein exhibit decreased susceptibility to chemical carcinogen-induced lung tumor development and angiogenesis. This gene is located in a cluster of other matrix metalloproteinase genes on chromosome 9. [provided by RefSeq, Feb 2016]