Nphp3 (NM_172460) Mouse Untagged Clone

CAT#: MC216333

Nphp3 (untagged) - Mouse nephronophthisis 3 (adolescent) (Nphp3), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nphp3
• Synonyms: 3632410F03Rik; AI550417; C230078J01; D330020E01Rik; pcy
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216333 representing NM_172460
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCACAGCCTCGTCGCTGGTGAGTCCCACTGGCGGGGAGGTGATCGAGGACACGTACGGGGCAGGCG
  GCGGCGAGGCCTGCGAGATCCCGGTGGAAGTGAAACCCAAGGCCCGCCTGCTGCGCAGCTCGTTCCGCAG
  GGGCGCGGGAGCCGGGCCCGGGTCGCTGCCTCGCGCGGCAGGGGGCGGCGGGCTGCTGGGCGCCAGCTTC
  AAGTCCACCGGCTCGTCTGTGCCCGAGCTAGAGTATGCGGCGGCCGAGTTCGAGCGGCTCAAGAAAGAGT
  ATGAGATCTTCCGGGTCAGCAAGAACCAGGAGTTGCTGTCCATGGGCCGCCGAGAGGCCAAGCTGGACAC
  GGAGAACAAGCGGCTGCGGGCGGAGCTGCAGGCACTTCAGAAAACTTACCAGAAGATACTTCGGGAAAAA
  GAGGGTGCGTTAGAAGCAAAATACCAAGCCATGGAGAGAGCGGTCACATTTGAACATGACAGAGACAGGG
  TTAAAAGGCAATTCAAGATTTTTAGGGAAACCAAAGAAAATGAAATTCAGGACTTACTGAGGGCCAAGCG
  AGAGTTGGAGAGCAAACTTCAGAGGCTGCAGGCTCAGGGGATCCAAGTGTTCGACCCTGGGGAGTCTGAC
  TCGGATGACAACTGCACAGATGTCACTGCTGCGGGAACGCAATGTGAGTACTGGGCCAGCAGAGCCCTGG
  GCAGCGAGCATTCCATAGGCAGCATGATCCAGCTCCCGCAGCCTTTCAGAGGCCCCGAGTTCGCCCACAG
  TTCTATAGATGTGGAAGGACCCTTTGCAAACATCAACCGAGATGACTGGGACGCTGCTGTAGCTGGTTTA
  TTACAGGCCACACCCTTGTTCTCACATTCCCTGTGGAGTCACCCTGTCAGGTGCTACCTCATCTACACAG
  ATGAAACACAGCCCGAAATGGAACTGTTCCTTAAGGACCATTCACCTAAACTTAAAAGAATGTGCGAGAC
  AATGGGATATTTCTTCCTCGCTGTTTATTTTCCATTAGATGTTGAAAATCAGTATCTCACTGTAAGAAAA
  TGGGAAATTGAGAAGAGTTCTTTAGTTATTTTATTCCTTCACTCAACATTACCCAGTTTTTTACTGGAAG
  ACTGTGAAGAAGCCTTCCTGCAAAACCCAGAAGGAAAGCCAGGATTAATCTACCACCGTTTGGAGGATGG
  GAAAGTCACTTGTGACTCTGTCCAGCAGTTCCTGGATCAAGTTTCCAACCTAGGCAAGACCACTAAAGCC
  AAGATCATTGAACACTCCGGAGATCCTGCAGAGGGAGTGTGTAAGATTTACGTGGGTGTGGAAAAGATTA
  TTAAACAGGTAAATATGCGTTTCTCTGTTAAGTTTTCATCTAAAATAGCTTCATGGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_172460
• Insert Size: 1389 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172460.1, NP_766048.1
• RefSeq Size: 2107 bp
• RefSeq ORF: 1389 bp
• Locus ID: 74025
• UniProt ID: Q7TNH6
• Cytogenetics: 9 56.11 cM
• Gene Summary: Required for normal ciliary development and function. Inhibits disheveled-1-induced canonical Wnt-signaling activity and may also play a role in the control of non-canonical Wnt signaling that regulates planar cell polarity. Probably acts as a molecular switch between different Wnt signaling pathways. Required for proper convergent extension cell movements.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses a different splice site in the 5' CDS and lacks all of the downstream CDS, compared to variant 1. The resulting protein (isoform b) is much shorter and has a distinct C-terminus when it is compared to isoform a.