Gpr22 (NM_175191) Mouse Untagged Clone

CAT#: MC216331

Gpr22 (untagged) - Mouse G protein-coupled receptor 22 (Gpr22), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gpr22
• Synonyms: 2900068K05Rik; AW061316
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216331 representing NM_175191
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGAATTGTCAATGACATCATGTATCAATTTGTGGAAAACTACCATGGTGAGCCCAAAGGCCCATA
  AAGCAACAGTAAACAGGCAAAACACCAACTGCTCCAAAAGAATGTGTTTTTCTCCTGTTCTGGAAATCAA
  CATGCAGTCTGAATCAAACGTCACGGTGCGAGATGACATTGATGACATCGACACCAATATGTACCAACCA
  CTGTCATACCCACTAAGCTTTCAAGTGTCTCTCACTGGATTTCTCATGTTAGAGATCGTGCTGGGGCTTG
  GCAGCAACCTTACCGTCCTGGTACTTTACTGCATGAAATCCAACTTAATCAACTCTGTCAGTAACATTAT
  TACAATGAACCTCCATGTACTTGATGTCATAATTTGTGTGGGATGCATTCCTCTAACTATAGTGATCCTT
  CTGCTCTCACTGGAGAGTAACACTGCTCTCATCTGCTGTTTCCACGAAGCTTGTGTTTCCTTTGCAAGTG
  TTTCGACAGCAATCAACGTTTTTGCTATTACTCTGGACAGATATGACATCTCTGTAAAACCTGCAAACAG
  AATTCTGACAATGGGCAGAGCTGTAATGCTAATGACATCCATTTGGATTTTTTCTTTCTTCTCATTCCTG
  ATTCCCTTCATTGAAGTAAATTTTTTCAGTCTTCAAAGTGGAAATACATGGGCAAACAAGACACTGCTGT
  GTGTCAGTACAAGTGAATACTATACTGAGCTCGGGATGTACTATCACCTTTTGGTGCAGATCCCCATCTT
  CTTCTTCACAGTTATAGTCATGTTGATCACATACACTAAGATACTCCAGGCTCTTAACATCCGCATAGGC
  ACTAGATTCTCAACAGGACAGAAGAAGAAAGCCCGAAAGAAAAAGACAATCTCTCTAGCTACACATGAGA
  CCACAGACATGTCACAAAGCAGTGGTGGGAGGAATGTCGTGTTTGGTGTGAGAACTTCAGTTTCTGTAAT
  AATTGCCCTCCGGCGAGCCGTGAAACGCCACCGGGAACGACGAGAACGGCAGAAAAGAGTCTTCAAAATG
  TCGTTATTGATTATTTCTACATTTCTTCTCTGTTGGACACCAATTTCTGTTTTAAATACCACCATTCTAT
  GTTTAGGCCCAAGTGACCTTTTAGTAAAATTAAGATTGTGTTTTCTAGTCATGGCTTATGGAACAACGAT
  ATTCCACCCTCTCTTGTATGCATTCACCAGACAAAAGTTTCAAAAGGTCTTAAAGAGTAAGATGAAAAAG
  CGAGTTGTTTCCATAGTTGAAGCTGATCCCATGCCTAATAACGCTGTAATACACAACTCATGGATAGATC
  CTAAAAGAAACAAAAAGGTTACCTATGAAGACAGTGAAATAAGAGAGAAATGTTTAGTACCTCAGGTTGT
  CACAGACTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_175191
• Insert Size: 1410 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175191.4, NP_780400.2
• RefSeq Size: 4587 bp
• RefSeq ORF: 1410 bp
• Locus ID: 73010
• UniProt ID: Q8BZL4
• Cytogenetics: 12 A3
• Gene Summary: Orphan G-protein coupled receptor. Seems to act through a G(i)/G(o) mediated pathway (By similarity). May be involved in ciliogenesis (By similarity).[UniProtKB/Swiss-Prot Function]