Chn2 (NM_001163640) Mouse Untagged Clone

CAT#: MC216325

Chn2 (untagged) - Mouse chimerin (chimaerin) 2 (Chn2), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Chn2
• Synonyms: 1700026N20Rik; 4930557O16Rik; ARHGAP3; Bch
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216325 representing NM_001163640
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCATCCAGCAACTCTAGTCTGTCCGGCTCCTCCGTGTCCTCTGATGCTGAGGAATACCAGCCTC
  CAATATGGAAATCATACTTGTATCAGCTGCAGCAAGAGGCACCTCGTCCCAAGAGGATCATTTGTCCCCG
  GGAGGTGGAAAACAGACCAAAATATTATGGAAGAGAGTTTCATGGGATCATCTCTCGGGAGCAGGCTGAT
  GAGCTTCTAGGCGGCGTGGAAGGTGCCTACATCCTTCGAGAAAGCCAGCGGCAGCCAGGATGCTACACGC
  TGGCACTGAGGTTTGGGAACCAGACCTTAAACTACCGGCTCTTCCACGACGGGAAGCACTTTGTGGGTGA
  GAAGAGGTTCGAATCCATTCACGACCTGGTGACAGATGGCTTGATCACACTCTACATAGAGACCAAGGCT
  GCTGAGTACATCGCGAAAATGACCACAAATCCCATCTACGAGCACATCGGATACGCCACTCTCCTCAGAG
  AAAAAGTGTCCCGAAGGCTGAGCAGGTCTAAAAACGAATCAAGGAAAGCTAGCGTCAGCAACGAGGAGCA
  CACGCCAGTGGAGAAGATCTCTTCACTGGTCCGAAGAGCTGCCCTCACACACAACGACAACCACTTCAAC
  TATGAGAAGACACACAACTTCAAGGTGCACACATTCCGGGGCCCACACTGGTGTGAATACTGTGCCAATT
  TTATGTGGGGGCTCATCGCCCAGGGAGTCCGGTGTTCAGATTGTGGGTTAAACGTACACAAACAGTGCTC
  CAAGCACGTCCCCAATGACTGCCAGCCAGATCTCAAGAGGATCAAGAAAGTGTACTGCTGTGACCTCACC
  ACGCTTGTCAAAGCTCACAACACTCAGAGACCCATGGTGGTGGACATATGTATTCGGGAAATTGAAGCAA
  GAGGATTAAAGTCAGAAGGGCTTTACAGAGTCTCTGGATTCACAGAACACATTGAAGATGTCAAGATGGC
  GTTTGACAGAGATGGTGAAAAGGCGGATATCTCTGCCAACATCTACCCAGACATAAACATCATCACTGGA
  GCCCTGAAACTGTACTTCCGAGACTTACCCATCCCTATCATCACATATGACACCTACTCCAAATTTATAG
  AGGCAGCAAAAATCTCCAATGCGGATGAGAGGCTGGAAGCTGTCCATGAGGTCCTGATGCTGCTGCCTCC
  TGCCCACTATGAGACCCTGCGCTACCTGATGATCCACCTGAAAAAGGTTACCATGAATGAAAAGGACAAT
  TTAATGAATGCCGAAAATCTGGGGATCGTGTTTGGGCCCACCCTGATGAGACCCCCTGAGGACAGCACCC
  TCACCACCCTCCATGACATGCGGTACCAAAAGCTGATCGTGCAGATTTTAATAGAAAACGAAGATGTTCT
  GTTTTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001163640
• Insert Size: 1407 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163640.1, NP_001157112.1
• RefSeq Size: 3150 bp
• RefSeq ORF: 1407 bp
• Locus ID: 69993
• Cytogenetics: 6 B3
• Gene Summary: GTPase-activating protein for p21-rac.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses an alternate, upstream transcription initiation site, uses a distinct start codon and contains several additional exons, compared to variant 1. The resulting protein (isoform 2, also referred to as beta2-chimerin) has a much longer and distinct N-terminus compared to isoform 1.