Lsm14a (NM_025948) Mouse Untagged Clone

CAT#: MC216316

Lsm14a (untagged) - Mouse LSM14 homolog A (SCD6, S. cerevisiae) (Lsm14a), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Lsm14a
• Synonyms: 2700023B17Rik; AA407828; AU017544; Tral
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216316 representing NM_025948
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGGGGGCACCCCTTACATCGGCAGCAAGATCAGCCTCATCTCCAAGGCGGAGATCCGCTACGAGG
  GCATCCTCTACACCATCGACACCGAGAACTCCACGGTAGCCCTCGCCAAAGTTCGATCCTTCGGTACAGA
  AGACAGACCAACAGATCGTCCAATACCGCCTCGAGATGAAGTCTTTGAATATATTATATTCCGCGGGAGT
  GATATTAAAGACCTCACTGTTTGTGAGCCACCCAAACCACAATGTTCTTTGCCTCAGGACCCGGCTATTG
  TTCAGTCTTCACTAGGTTCGTCGTCATCTTCATTCCAGTCGGTGGGTTCCTATGGACCTTTTGGCAGGAT
  GCCAGCATACAGTCAGTTCAGTCCAAGTACATTAGTTGGGCAGCAGTTTGGTGCTGTTGGTGTTGCTGGA
  AACTCTTTGACATCCTTTGGAACAGAAGCATCAAATAGTGGTACCTTATCCCAGAGCAATGCAGTTGGTT
  CGGCCTTCACACAAGACACAAGATCTGTAAAACCACAGCTAGCCCAAGGTCGATCAAGCCCTCAGTTAGA
  CCCTTTGAGAAAAAGTCCAACCATGGAACAAGCAGTACAGACCGCCTCGGCCCACTTACCTGCTCCAGCA
  CCTGTTGGGAGAAGGAGCCCTGTACCAGCCAGGCCTTTGCCACCTACCAGCCAGAAAGCAATAGACAACC
  AAGAGCACAGGCGAGCTGAAGTACACAAAGTCCCAAGGCCAGAAAATGAGCAACTCAGAAATGACAAGAG
  GCAAGTAGTTCCTGGAGTTCCTTCTGCTCCAAGAAGAGGACGTGGGGGTCATCGAGGTGGCAGGGGAAGA
  TTTGGTATCCGTCGAGATGGTCCAATGAAATTTGAGAAAGACTTTGACTTTGAAAGTGCAAATGCACAAT
  TTAACAAGGAGGAAATTGACAGAGAGTTTCATAATAAACTTAAACTGAAAGAAGATAAACTTGAGAAACA
  AGAGAAACCTGTAAACGGTGAAGACAAAGGAGACTCAGGAGTCGACACTCAGAACAGTGAAGGGAATGCT
  GATGAGGAAGATCCCCTTGGGCCCAACTGCTACTATGACAAGACCAAGTCCTTCTTTGACAACATCTCTT
  GTGATGATAATAGAGAAAGGAGACCAACTTGGGCTGAAGAAAGAAGGTTAAATGCTGAAACTTTTGGCAT
  CCCGCTTCGTCCAAACCGTGGCCGTGGAGGCTACCGAGGCAGAGGAGGCCTTGGCTTCCGTGGTGGCAGA
  GGGCGTGGTAGTGGCCGAGGAGGAACCTTCACTGCCCCTCGAGGGTTTCGTGCTGGATTCAGAGGCGCTC
  GTGGTGGTAGGGAGTTTGCAGACTTTGAATATAGGAAAACCACGGCTTTTGGACCCTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_025948
• Insert Size: 1389 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025948.2, NP_080224.1
• RefSeq Size: 2724 bp
• RefSeq ORF: 1389 bp
• Locus ID: 67070
• UniProt ID: Q8K2F8
• Cytogenetics: 7 B1
• Gene Summary: Essential for formation of P-bodies, cytoplasmic structures that provide storage sites for non-translating mRNAs.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.