Hsf4 (NM_011939) Mouse Untagged Clone

CAT#: MC216292

Hsf4 (untagged) - Mouse heat shock transcription factor 4 (Hsf4), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Hsf4
• Synonyms: HSF 4; HSTF 4; HSTF4; ldis1; mHSF4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216292 representing NM_011939
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGGAAGCGCCAGCTGCGCTGCCCACGGAGCCAGGCCCCAGCCCGGTACCTGCCTTCCTCGGCAAGC
  TATGGGCGCTGGTAGGCGACCCAGGCACCGACCACCTCATCCGCTGGAGCCCGAGTGGCACCAGCTTCCT
  CGTAAGTGATCAGAGCCGCTTCGCCAAGGAAGTGCTGCCCCAGTACTTCAAGCACAGCAACATGGCGAGC
  TTTGTTCGCCAACTCAACATGTATGGTTTTCGGAAGGTGGTGAGCATCGAGCAAGGTGGCCTGCTCAGAC
  CAGAGCGTGACCACGTTGAGTTTCAGCATCCGAGCTTCGTGCGAGGCCGCGAGCAGCTACTGGAGCGCGT
  GCGCCGCAAGGTACCTGCGCTGCGAGGCGATGACAGTCGATGGCGTCCGGAAGACCTGAGCCGACTGCTG
  GGAGAGGTGCAAGCTTTGAGAGGAGTGCAGGAGAGCACGGAGGCACGGCTGCAGGAACTCAGGCAGCAGA
  ACGAGATCTTGTGGCGAGAGGTGGTAACTTTGCGGCAGAGCCACAGTCAGCAGCACCGGGTCATCGGCAA
  GCTAATCCAGTGCCTGTTTGGGCCACTTCAGACAGGGCCCAGCAGTACAGGAGCCAAGAGAAAACTGTCC
  CTAATGCTAGATGAGGGGAGCGCCTGTTCAGCATCTGCCAAATTCAATGCCTGCCCTGTGTCTGGTGCTC
  TCCTCCAGGACCCCTACTTTATCCAGTCGCCTTCTCCTTGCAGCCCCTCCCAGAGACCACGTTGGGCCTC
  AGCCCTCACAGGGCCAGAGGGCCCATCATCTCTGACATCCCAGAAGATTCTCCATCTCCTGAAGGACACA
  GGCTTTCTCCCTCCGGTGGTTGCAGGAGCACCCCCACCGCTGCCCGTGGCTGTGGTGCAGGCCATCCTGG
  AAGGGAAAGGGAGCTACAGCCCTGAGGGGCCCAGGAGTGTACAACAGCCTGAACCAAGGGGCCCCAGGGA
  GGTACCTGACAGGGGAACTCTGGGCCTGGATCGGGGTAACCGGAGCCCAGAGAGTCTGCTACCCCCAATG
  CTGCTTCGGCCTGCCCCTGAAACTCTGGAGCCCGTGGCACCTGTGGATGTGCTCGGCCCTAGCCTGCATG
  GACGAGAATGGACCTTGATGGATCTGGACATGGAGTTGTCTCTGATGCAGCCCTTGGCACCAGAGACGGA
  TGAAGCAGAGCTGACGGTCAAGGAGTTGAATTCTTCTGGTGTAGGGAAAGACCACACACTGGGAACTCCA
  CTCATGCTAGATGTCCAGGCGGATTTGGAGGGTGCAGCCCTGTCTGTGCCTGGGGCTTTAACCCTGTACA
  ACGTCACCGAGAGCAACGCCTCCTACTTGGATCCTGGAGCCAGTCCCTCCTCTCCCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_011939
• Insert Size: 1389 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011939.2, NP_036069.1
• RefSeq Size: 1649 bp
• RefSeq ORF: 1389 bp
• Locus ID: 26386
• UniProt ID: Q9R0L1
• Cytogenetics: 8 D3
• Gene Summary: DNA-binding protein that specifically binds heat shock promoter elements (HSE). The HSF4A isoform represses transcription while the HSF4B isoform activates transcription.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) uses two alternate in-frame splice sites in that result in a frameshift in the central coding region, compared to variant 1. The encoded isoform (2) is shorter and has a distinct internal segment, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.