Ctsc (NM_009982) Mouse Untagged Clone

CAT#: MC216251

Ctsc (untagged) - Mouse cathepsin C (Ctsc), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Ctsc
• Synonyms: AI047818; CatC; D; DP; DPP1; DPPI
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216251 representing NM_009982
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTCCCTGGACCCACTCCTTGCGCGCCGTCCTGCTGCTGGTGCTTTTGGGAGTCTGCACCGTGCGCT
  CCGACACTCCTGCCAACTGCACCTACCCTGATCTGCTGGGCACCTGGGTGTTCCAGGTGGGCCCTAGAAG
  TTCCCGAAGCGACATTAACTGCTCGGTGATGGAAGCAACAGAAGAAAAGGTAGTGGTACACCTTAAGAAG
  TTGGATACTGCCTACGACGAGCTGGGCAATTCCGGGCATTTTACCCTCATTTACAACCAAGGCTTCGAGA
  TTGTGTTGAATGACTACAAATGGTTTGCGTTTTTCAAGTATGAAGTCAGAGGCCACACAGCTATCAGTTA
  CTGCCATGAGACCATGACTGGGTGGGTCCATGATGTGCTGGGCCGGAACTGGGCTTGCTTTGTTGGCAAG
  AAGGTGGAAAGTCACATTGAGAAGGTTAATATGAATGCAGCACATCTTGGAGGTCTCCAGGAAAGATATT
  CTGAAAGACTCTACACTCACAACCACAACTTTGTGAAGGCCATCAATACCGTTCAGAAGTCTTGGACTGC
  AACTGCATATAAGGAATATGAGAAAATGAGCCTGCGAGATCTGATAAGGAGAAGTGGCCACAGCCAAAGG
  ATCCCAAGGCCCAAACCTGCCCCGATGACTGATGAAATACAGCAACAAATTTTAAATTTGCCAGAATCTT
  GGGACTGGAGAAACGTCCAAGGCGTCAATTATGTTAGCCCTGTTCGAAACCAAGAATCTTGTGGAAGCTG
  CTACTCATTTGCCTCTATGGGTATGCTAGAAGCAAGAATTCGTATATTAACCAACAATTCTCAGACACCA
  ATCCTGAGTCCTCAGGAGGTTGTATCTTGCAGCCCCTATGCCCAAGGTTGTGATGGTGGATTCCCATACC
  TCATTGCAGGGAAGTATGCCCAAGATTTTGGGGTGGTGGAAGAAAGCTGCTTTCCCTACACAGCCAAAGA
  TTCTCCATGCAAACCAAGGGAGAATTGCCTCCGTTACTATTCTTCTGACTACTACTATGTGGGTGGTTTC
  TATGGTGGCTGCAATGAAGCCCTGATGAAGCTTGAGCTGGTCAAACATGGACCCATGGCAGTTGCCTTTG
  AAGTCCACGATGACTTCCTACACTACCACAGTGGAATCTATCACCACACTGGGCTGAGTGACCCTTTCAA
  CCCCTTCGAGCTGACAAATCATGCTGTTTTGCTTGTGGGCTATGGAAGAGATCCAGTTACTGGGATAGAA
  TACTGGATTATAAAGAACAGCTGGGGCTCTAACTGGGGGGAGAGTGGCTACTTCCGTATCCGCAGAGGAA
  CTGATGAATGTGCAATTGAGAGTATAGCCGTGGCGGCCATACCGATTCCTAAATTATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_009982
• Insert Size: 1389 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC067063, AAH67063
• RefSeq Size: 1866 bp
• RefSeq ORF: 1389 bp
• Locus ID: 13032
• UniProt ID: P97821
• Cytogenetics: 7 D3
• Gene Summary: This gene encodes a member of the peptidase C1 (papain) family of cysteine proteases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate multiple protein products. These products include the dipeptidyl peptidase 1 light, heavy, and exclusion domain chains, which together comprise one subunit of the homotetrameric enzyme. This enzyme has amino dipeptidase activity and may play a role in the activation of granzymes during inflammation. Homozygous knockout mice for this gene exhibit impaired granzyme activation and enhanced survival in a sepsis model. [provided by RefSeq, Aug 2015]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.