Chrm2 (NM_203491) Mouse Untagged Clone

CAT#: MC216243

Chrm2 (untagged) - Mouse cholinergic receptor, muscarinic 2, cardiac (Chrm2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Chrm2
• Synonyms: AChR-M2; Chrm-2; M2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216243 representing NM_203491
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATAACTCAACTAACTCCTCGAACAATGGTTTGGCTATTACCAGTCCTTACAAGACATTTGAAGTGG
  TATTTATTGTCCTTGTGGCTGGATCCCTCAGTCTGGTGACCATCATTGGGAACATTCTAGTCATGGTTTC
  CATTAAAGTCAACCGCCACCTTCAGACTGTCAACAATTACTTCTTGTTCAGCCTGGCCTGTGCTGACCTC
  ATCATAGGTGTTTTCTCCATGAACTTGTATACCCTCTACACTGTGATTGGCTACTGGCCTTTGGGACCTG
  TAGTGTGCGACCTTTGGCTAGCCTTGGACTATGTTGTCAGCAATGCCTCCGTTATGAATCTTCTCATCAT
  CAGCTTTGATAGATACTTCTGTGTCACAAAACCTCTAACCTACCCAGTTAAGCGGACCACAAAAATGGCA
  GGCATGATGATTGCAGCTGCGTGGGTTCTTTCCTTCATCCTCTGGGCCCCAGCCATTCTCTTCTGGCAGT
  TCATCGTAGGGGTAAGGACTGTGGAAGACGGGGAGTGCTACATTCAGTTCTTTTCCAACGCTGCCGTCAC
  CTTTGGCACTGCCATTGCGGCTTTCTATCTGCCTGTCATCATCATGACTGTGCTCTATTGGCACATATCC
  CGGGCGAGCAAGAGCAGAATAAAGAAAGAAAAGAAGGAACCAGTGGCCAACCAAGACCCGGTGTCTCCGA
  GTCTAGTGCAAGGAAGAATTGTAAAGCCAAACAACAACAACATGCCTGGTGGTGATGGTGGCCTGGAGCA
  CAACAAGATCCAGAATGGCAAGGCTCCGCGGGACGGTGGGACTGAAAACTGCGTTCAGGGGGAGGAGAAA
  GAAAGCTCCAACGACTCCACGTCTGTCAGTGCCGTGGCCTCCAACATGAGAGATGATGAGATAACCCAGG
  ATGAAAACACGGTTTCCACTTCCCTGGGCCACTCCAAAGATGACAACTCTAGGCAGACATGCATCAAAAT
  TGTCACCAAGACCCAAAAGGGTGACGCATGCACACCAACAAGTACCACAGTAGAACTAGTGGGATCGTCA
  GGTCAGAATGGTGATGAAAAGCAGAACATTGTAGCCCGCAAAATTGTGAAGATGACCAAGCAGCCTGCCA
  AAAAGAAGCCTCCTCCATCCCGGGAAAAGAAAGTGACCAGGACAATCTTGGCTATCCTGTTGGCTTTCAT
  CATCACGTGGGCGCCATACAATGTCATGGTGCTCATCAATACCTTCTGTGCACCCTGCATCCCCAATACA
  GTGTGGACAATTGGCTACTGGCTCTGTTACATTAATAGCACCATCAACCCTGCCTGCTATGCACTTTGTA
  ACGCCACCTTCAAAAAGACTTTTAAGCACCTCCTTATGTGTCATTACAAGAACATAGGCGCTACAAGGTA
  A
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_203491
• Insert Size: 1401 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_203491.1, NP_987076.1
• RefSeq Size: 2036 bp
• RefSeq ORF: 1401 bp
• Locus ID: 243764
• UniProt ID: Q9ERZ4
• Cytogenetics: 6 B1
• Gene Summary: The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition. Signaling promotes phospholipase C activity, leading to the release of inositol trisphosphate (IP3); this then triggers calcium ion release into the cytosol (By similarity).[UniProtKB/Swiss-Prot Function]