Nuf2 (NM_023284) Mouse Untagged Clone

CAT#: MC216225

Nuf2 (untagged) - Mouse NUF2, NDC80 kinetochore complex component, homolog (S. cerevisiae) (Nuf2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Nuf2
• Synonyms: 2410003C07Rik; AU044112; C85691; Cdca1; NUF2R
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216225 representing NM_023284
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAACTTTGTCATTCCCCAGATACAATGTAGCTGAGCTTGTGGTTCATATTCGCAATAAATTATTAA
  CAGGAGCAGATGGCAAAAACCTCTCGAAGAGCGATTTTCTTCCAAATCCGAAGTCTGATGTCTTGTACAT
  GATTTACATGAAAGCCTTACAGTTAGTGTATGGAGTCCGGCTGGAGCACTTCTACATGATGCCGATGAAC
  ATAGAAGTCACATATCCACATTTAATGGAGGGCTTCTTACCAGTCAGAAGTTTGTTCTTTTACATGGATT
  CCTTTATGCCCATTTGCCGGGTGAATGACTTTGAGATTGTCGACATTCTTAATCCAAGAACAAACCGGAC
  AAGTCGTTTTTTAAGTGGCATTATCAACTTTATTCACTTCAGAGAAACGTGCCTGGAGAAGTGTGAAGAA
  TTTCTTTTGCAAAATAAATCCTCTATGGTCAGAATGCAGCAGTTAAGCAATGTGCACCAGGAAGCTTTGA
  TGAAACTGGAAAAACTCAATACCGTTCCGGCTGAGGAGCGAGAGGAGTTCAAGCAGTTTATGGATGACAT
  TCAGGAGCTGCAGCACCTGCTGAATGAAGAGTTCAGACAGAAAACGACACTGCTGCAGGAGGAATATGCC
  AAAATGAAATCCGATATTTCAGAGAAAACCAAGCATTTAAATGAACAAAAGTTGTCATTAGTTTCTTTGA
  AAGAAGTTGAAGACAATTTGAAAAGCAAAATTGTGGACTCTCCAGAGAAGCTGAAGAACTATAAAGACAA
  GATGAAAGGCACCGTCCAGAAGCTCCGCAGTGCCAGGGAAAAAGTGATGGAGCAGTATGATATCTATAGA
  GATTCTGTGGATTGCTTGCCTTCCTGTCAGCTGGAGGTGCAGTTGTATCAAAAGAAATCACAGGACCTTG
  CAGATAATAGGGAGAAACTAAGCAGTCTCTTAAAGGAGAGCTTGAACTTGGAGGACCAGATTGAAAGTGA
  TTCATCGGAACTAAAGAAACTGAAGACTGAAGAAAATTCTCTCATAAGAATGACGACTGTAAAGAAGGAG
  AAGCTCGCCACCGCGCGATTCAAAATCAACAAGAAGCAGGAGGATGTGAAACATTACAAGCAGGCCATGA
  TTGAAGACTGCAATAAAGTTCAAGAAAAGAGAGATGCTGTCTGTGAGCAAGTAACCACTGTTAATCAAGA
  AATCCACAAGATTAAATCTGCAATTCAACAACTGAGAGATACCAAGAAAAGGGAGATATTGAAGTCTCAG
  GAGATCTTTGTAAACCTGAAAAGTGCTCTGGAGAAGTACCATGAGGGCATCGAGAAGGTAGCGGAGGAGC
  GCAGCGCTAAACTGGAGGAGAAGACGGCAGAGCTGAAGAAGAGGATGGTCAGAATGGTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_023284
• Insert Size: 1392 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023284.3, NP_075773.2
• RefSeq Size: 2405 bp
• RefSeq ORF: 1392 bp
• Locus ID: 66977
• UniProt ID: Q99P69
• Cytogenetics: 1 H2.3
• Gene Summary: Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules.[UniProtKB/Swiss-Prot Function]