Mief2 (NM_001009927) Mouse Untagged Clone

CAT#: MC216198

Smcr7 (untagged) - Mouse Smith-Magenis syndrome chromosome region, candidate 7 homolog (human) (Smcr7), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Mief2
• Synonyms: AI482195; Gm11; Smcr7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216198 representing NM_001009927
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGAGTTCTCCCAGAAGCAGAGGAAACAGAGCGGCAGCGAGGGCCTGGGCAGTGTGGTGGACTTCC
  TCTTGGCTAATGCTCGATTGGTGCTAGGTGTGGGCGGGGCTGCAGTGCTGGGCATTGCTACCCTGGCTGT
  AAAGCGGCTCATCGACAGGGCCACCAGCCCTCCAGATGAGGATGACACCAAGGGGGACAGCTGGAAGGAA
  CTGAGCCTGCTGAGAGCCACATCGCCCCAAAAGCCCCAACCCCCTCCTGCTGCCTTCAGCCAGCCCCTAG
  CTACTGGGTCCCCCTCCCCCTCTGTGCCAGTGGAGCCCACGCCCATTCATTCTCCCACGACACCTAAGTT
  CAGCACTATAGCACCCCTGTGCCTGACGTTCCAGGAGAGGCTGCTGGCCTTCGAGCGCAAGCATGTGATT
  ACCCCAGAAGCCCACGTGACGTTGGCGAAACAGCTGGCTGGAGACATTGCCTTGGAGCTGCAGGCCTACT
  TGAGGAGCAAGTTCCCAGAACTGCCCTTTGGTGCACTCGTGCCGGGTGGGCCTCTCTACGACGGGCTCCA
  GGCAGGGACTGCTGAGCACGTGCGCCTGCTGGCGCCTCTGGAGTTGGAGCCGGGGCTATGGAGCCTGGTA
  CCTGGCGTGGACACTGTGGCCAGGGAGCCTCGATGCTGGGCTGTACGCAGGACTCAGCTTGAGTTTCATC
  CCCGTGGGTGCAGCCCATGGGACCGCTTCCTGGTGGGGGGCTACCTCTCCTCCCGAGTCTTGCTGGAGCT
  GCTACGGAAGGCCCTGTCAGCCTCTGTCAACTGGCCAGCCATTGGCAGCCTGCTCGGGTGTCTGATCTGG
  CCCGATGTGGCTTCGGAGGAGCTGCTGCTCAAAGTGCAACACGAGTGCCTGGAGTTCACTTTAGCTGTGC
  TCATGGTAGTCCCTGGGGCCAGCACTGATGACCGCCTGCTGCTGGCTTGGCCCCTAGAGGGGCTGGCCAG
  CAATCTCTGGCTGCAGGATCTATATCCAGTAGAGACCGCCCGTTTGCGGGCCCTGGATGACCAGGATGCT
  GGTACTCGGCGGAGGTTGTTGCTGCTGCTGTGTGGTATCTGTCGTGGCCATCCGGCTCTAGTGCGGCTGG
  GCTGGAGCCACCTGACTCAGGTGGTTCTGCATCTGGGTGAAGAGGAAGTGGCCTGGACTGAGGAGGCCTT
  GGGGGAACGGTTTCTGCAAGCCCTGGAGTTTCTGGTGGGCAGCTTGGAGCAGGCTAGCCTGCCCTGTCAC
  TTCAACCCCAGTGTGAACCTCTTGGGTAACTTCCGGGAAGAGGAGATTGATGACATTGGCTACGTGCTGT
  ACAGCGGCCTCCAGGTTCCTGAAAGCCTATTCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001009927
• Insert Size: 1365 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001009927.2, NP_001009927.1
• RefSeq Size: 2564 bp
• RefSeq ORF: 1365 bp
• Locus ID: 237781
• UniProt ID: Q5NCS9
• Cytogenetics: 11 B2
• Gene Summary: Mitochondrial outer membrane protein which regulates mitochondrial organization. It is required for mitochondrial fission and promotes the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface independently of the mitochondrial fission FIS1 and MFF proteins. Regulates DNM1L GTPase activity.[UniProtKB/Swiss-Prot Function]