Cyp2c23 (NM_001167905) Mouse Untagged Clone

CAT#: MC216193

Cyp2c44 (untagged) - Mouse cytochrome P450, family 2, subfamily c, polypeptide 44 (Cyp2c44), transcript variant 2, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cyp2c23
• Synonyms: AW107714; b2b386Clo; BC034834; Cyp2c44
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216193 representing NM_001167905
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTGCTGGGTCTCCCTACGCTTGCTCTCCTGGTTTTGGTGATGTCCCTCAGCCTCCTTTCAGTGT
  GGACAAAAATGCGCACTGGGGGGAGACTGCCCCCTGGCCCCACTCCACTTCCCATTATTGGGAATATACT
  GCAATTGGACCTCAAAGACATCCCTGCATCGCTTTCCAAGCTGGCCAAAGAGTATGGGCCTGTTTACACT
  CTGTACTTTGGATCCTGGCCTACCGTGGTCCTACATGGCTATGATGTGGTGAAGGAAGCGCTACTCAATC
  AAGGTGATGAGTTCCTTGGGAGAGGACCTCTGCCCATTATCGAAGATAGCCAGAAGGGACATGGAATTGT
  TTTCAGTGAAGGAGAGAGGTGGAAACTCCTGCGACGCTTCTCCCTCATGACGCTGAAGAACTTCGGAATG
  GGGAAGAGAAGCCTAGAGGAGAGGGTGCAGGAGGAAGCCCGGTGCCTCGTGGAGGAGTTACATAAAACAG
  AAGCTCAGCCCTTTGACCCCACCTTCATCCTGGCCTGTGCTCCCTGCAACGTGATCTGCTCCATCCTTTT
  CAACGAGCGATTCCCATACAATGACAAGACATTCCTCAACCTCATGGACCTTTTAAATAAAAATTTTTAC
  CAACTGAACTCTATATGGATCCAGATGTACAACCTATGGCCGACGATCATGAAGTACATACCTGGAAAGC
  ATAGAGAATTCTCAAAAAGGCTTGGTGGTGTTAAAAACTTCATCCTAGAAAAAGTGAAGGAGCACCAGGA
  GTCCCTGGACCCCGCCAACCCTCGGGATTACATCGACTGTTTCCTCAGCAAGATCGAGGAGGAGAAACAC
  AACCTGAAATCTGATTTTAATCTGGAGAATTTGGCCATCTGTGGGTCTAACTTGTTTACGGCAGGCACAG
  AGACAACCAGCACCACCCTGAGATTCGGGCTTCTGCTCCTTGTCAAGCATCCAGAGGTGCAAGCCAAAGT
  TCATGAAGAACTTGATCGTGTGATTGGACGCCACCAACCCCCCAGCATGAAGGACAAGATGAAGCTGCCC
  TATACAGATGCCGTGCTGCATGAGATTCAAAGATACATCACTCTCCTTCCTTCTAGTCTGCCCCATGCTG
  TGGTCCAGGACACAAAATTCAGACACTATGTCATCCCCAAGGGCACCGCTGTGTTCCCGTTTCTGTCTTC
  CATCTTGTTGGATCAAAAGGAGTTTCCCAACCCAGAGAAGTTTGATCCAGGACACTTTCTGGATAAAAAT
  GGCTGCTTCAAGAAAACAGACTACTTTGTTCCTTTCTCCCTCGGTAAATATACACGTTTTGGGCAACTCT
  TAGCGTCACTGAATTACGTCTTACGTGACCCACTGATGTAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001167905
• Insert Size: 1371 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001167905.1, NP_001161377.1
• RefSeq Size: 1596 bp
• RefSeq ORF: 1371 bp
• Locus ID: 226143
• UniProt ID: A0A1D5RLI1
• Cytogenetics: 19 C3
• Gene Summary: A cytochrome P450 monooxygenase involved in polyunsaturated fatty acids (PUFAs) metabolism and signaling (PubMed:15084647). Catalyzes preferentially the epoxidation of double bonds of PUFAs (PubMed:15084647). Converts arachidonoic acid primarily to stereospecific products (8R,9S)-epoxyeicosatrienoate (EET) and (11R,12S)-EET (PubMed:15084647). Via EETs may inhibit the epithelial sodium channels in nephron segments, leading to increased sodium excretion and stable systemic blood pressure (PubMed:24966089, PubMed:24368771). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed:15084647).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) differs in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.