Gramd1c (NM_153528) Mouse Untagged Clone

CAT#: MC216185

Gramd1c (untagged) - Mouse GRAM domain containing 1C (Gramd1c), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Gramd1c
• Synonyms: 4921521N14Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216185 representing NM_153528
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACACTTGTTATCAGTTGAAGAAAATGTGCAGCCAAGAAGTCCAGGAAGAAGCAGCGTGGATGACG
  CTGGGGAAAGAGATGAGAAGTTCTCCAAGGCCGTCAGCTTTACACAGGAGTCAGTTAGCAGAGCTTCAGA
  AACAGAGCCATTGGACGGGAACTCACCGAAAAGAGGACTAGGAAAAGAGGATTCCCAGAGCGAGAGAAAT
  GTGAGAAAAAGTCCTTCGCTAGCTTCAGAAAAGAGGATAAGCAGAGCACCCTCCAAGTCACTGGACTTGA
  ATAAGAATGAGTACCTTTCTCTGGATAAAAGCAGCACTTCAGATTCTGTGGACGAAGAAAATATTCCCGA
  GAAAGATCTTCAAGGAAGACTTTATATCAACCGTGTCTTTCACATCAGTGCTGAGAGAATGTTCGAACTG
  CTGTTCACTAGCTCACACTTTATGCAGAGATTTGCAAATTCTAGAAATATAATAGATGTGGTATCTACCC
  CCTGGACAGTTGAATCTGGAGGCAATCAGCTGCGAACCATGACCTATACCATAGTCCTCAGCAACCCGCT
  AACTGGGAAGTATACTGCTGCCACAGAAAAGCAGACCCTGTATAAAGAGAGCCGGGAAGCACAGTTCTAC
  CTGGTAGACTCCGAAGTGCTGACACATGATGTGCCCTATCACGACTACTTCTACACTTTGAACAGATACT
  GTATCGTGAGATCTGCAAAACAGAGATGCAGGCTGAGAGTCTCCACAGACTTGAAGTACAGGAAACAACC
  ATGGGGCCTTATCAAGTCTTTAATTGAGAAGAATTCCTGGAGTTCACTGGAGAGCTACTTCAAAAAGCTT
  GAATCCGATTTGTTAATGGAAGAGTCTGTGTTGAGTCAATCCATTGAAGATGCTGGAAAACATAGCAGCC
  TACGCCGGAGAAGGCGGACCTTGAACCGGACAGCAGAGCCCGTTCCAAAGCTGTCCTCTCAGCGCTCTTC
  CACAGATTTGGGCTTCGAGGCCAAAGTAGATGTTACAGGAAAGAGAAAGACCGTGGACAGTTATGACACC
  GCCCTTATTGTGGTGATGAGCATATTCTTGCTGCTGCTCGTTCTGCTGAATGTGACACTATTTCTGAAGC
  TGTCAAAGATAGAACACGCTACCCAGTCCTTCTACCAGCTTCACCTCCAGGGAGAAAAATCTTTAAATTT
  AGTCTCTGACAGGTTCTCAAGAACAGAAAATATTCAAAAGAACAAAGATCAGGCCCACCGCCTAAAAGGA
  GTACTCCAAGATTCCATAGTGATGTTGGAACAGCTGAAGAGCTCACTCATTATGCTTCAAAAAACCTTTG
  ATTTACTAAATAAGAACAAGTCTGGGGTGGCTGTGGAGAGCTAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_153528
• Insert Size: 1374 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153528.2, NP_705756.1
• RefSeq Size: 3506 bp
• RefSeq ORF: 1374 bp
• Locus ID: 207798
• UniProt ID: Q8CI52
• Cytogenetics: 16 B4
• Gene Summary: Cholesterol transporter that mediates non-vesicular transport of cholesterol from the plasma membrane (PM) to the endoplasmic reticulum (ER) (PubMed:30220461). Contains unique domains for binding cholesterol and the PM, thereby serving as a molecular bridge for the transfer of cholesterol from the PM to the ER (PubMed:30220461). Plays a crucial role in cholesterol homeostasis and has the unique ability to localize to the PM based on the level of membrane cholesterol (PubMed:30220461). In lipid-poor conditions localizes to the ER membrane and in response to excess cholesterol in the PM is recruited to the endoplasmic reticulum-plasma membrane contact sites (EPCS) which is mediated by the GRAM domain (PubMed:30220461). At the EPCS, the sterol-binding VASt/ASTER domain binds to the cholesterol in the PM and facilitates its transfer from the PM to ER (PubMed:30220461).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes isoform 1. Variants 1 and 2 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.