Celf4 (NM_001174074) Mouse Untagged Clone

CAT#: MC216182

Celf4 (untagged) - Mouse CUGBP, Elav-like family member 4 (Celf4), transcript variant 6, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Celf4
• Synonyms: A230070D14Rik; Brul4; BRUNOL-4; Brunol4; C130060B05Rik
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216182 representing NM_001174074
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTATATAAAGATGGCCACGTTAGCAAACGGACAGGCTGACAACGCGAGCCTCAGTACCAACGGGCTAG
  GCAGCAGCCCGGGCAGCGCCGGGCATATGAACGGATTAAGCCACAGCCCGGGGAACCCGTCGACCATTCC
  CATGAAGGACCACGATGCCATCAAGCTGTTCATTGGGCAGATCCCCCGAAACCTGGATGAGAAGGACCTC
  AAGCCCCTCTTCGAGGAGTTCGGCAAGATCTACGAGCTTACGGTTCTGAAGGACAGGTTCACAGGCATGC
  ACAAAGGCTGCGCTTTCCTCACCTACTGCGAGCGTGAGTCAGCGCTGAAGGCCCAGAGCGCGCTGCACGA
  GCAGAAGACCCTGCCCGGGATGAACCGGCCGATCCAGGTGAAGCCTGCGGACAGCGAGAGCCGAGGAGGT
  AGTAGCTGCCTGCGCCAGCCCCCTTCACAAGATAGAAAACTCTTCGTGGGTATGCTCAACAAGCAACAAT
  CTGAGGACGACGTGCGCCGCCTCTTCGAGGCCTTCGGGAACATCGAGGAGTGCACTATCCTGCGCGGGCC
  GGACGGCAACAGCAAGGGGTGCGCCTTTGTGAAGTACTCCTCCCATGCCGAGGCACAAGCCGCCATCAAC
  GCTCTACATGGCAGCCAGACCATGCCTGGAGCCTCCTCCAGCCTGGTGGTCAAGTTTGCAGACACTGACA
  AGGAGCGCACAATGCGACGGATGCAGCAGATGGCTGGCCAGATGGGCATGTTCAACCCCATGGCCATCCC
  CTTCGGAGCCTATGGCGCCTATGCTCAGGCAATGCAGCAGCAAGCAGCCCTCATGGCATCGGTCGCGCAA
  GGAGGCTACCTGAATCCCATGGCTGCCTTCGCTGCCGCCCAAATGCAGCAGATGGCGGCCCTCAACATGA
  ATGGCCTGGCAGCCGCACCTATGACCCCAACCTCAGGTGGCAGCACCCCTCCAGGCATCACTGCACCAGC
  TGTGCCTAGCATCCCATCCCCCATTGGGGTGAACGGCTTCACGGGCCTCCCCCCTCAGGCCAATGGGCAG
  CCTGCTGCGGAAGCTGTGTTTGCCAATGGCATTCACCCCTACCCAGCACAGAGCCCCACCGCAGCCGACC
  CCCTGCAGCAGGCCTACGCTGGAGTGCAGCAGTATGCAGGACCTGCCTACCCTGCTGCCTATGGTCAGAT
  TAGCCAGGCCTTTCCTCAGCCACCGCCAATGATTCCCCAGCAACAGAGAGAAGGCTTCGTGAGCTTCGAC
  AACCCGGCCAGCGCACAGACCGCCATCCAGGCCATGAACGGCTTCCAGATAGGCATGAAGAGGCTCAAGG
  TGCAGCTGAAGCGGCCCAAAGACGCCAATCGCCCGTACTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001174074
• Insert Size: 1371 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001174074.1, NP_001167545.1
• RefSeq Size: 3863 bp
• RefSeq ORF: 1371 bp
• Locus ID: 108013
• UniProt ID: Q7TSY6
• Cytogenetics: 18 A2
• Gene Summary: RNA-binding protein implicated in the regulation of pre-mRNA alternative splicing. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Promotes exclusion of both the smooth muscle (SM) and non-muscle (NM) exons in actinin pre-mRNAs. Activates the splicing of MAPT/Tau exon 10. Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (6) encodes isoform (51, also known as F). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.