Kremen2 (NM_028416) Mouse Untagged Clone

CAT#: MC216164

Kremen2 (untagged) - Mouse kringle containing transmembrane protein 2 (Kremen2), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Kremen2
• Synonyms: 2900054E04Rik; Krm2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216164 representing NM_028416
  Red=Cloning site Blue=ORF
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGACACCACATCTGCAGGGCTTCCTCCTCCTCTTCCCATTGCTGCTGCGGCTGCACGGGGCCTCAG
  CAGGGAGCCTGCACAGTCCAGGCCTGTCCGAATGCTTCCAGGTGAACGGCGCTGACTACCGAGGCCACCA
  GAACTACACCGGCCCACGCGGAGCTGGACGCCCTTGTCTTTTCTGGGACCAGACACAGCAGCACAGCTAC
  AGCAGCGCCAGCGACCCTCAGGGCCGCTGGGGGTTGGGTGCACATAACTTCTGTAGGAACCCAGACGGTG
  ATGTGCAGCCCTGGTGCTACGTGGCAGAGACAGAAGAGGGCATCTACTGGCGCTACTGTGATATCCCCAC
  ATGTCACATGCCTGGGTACCTGGGCTGCTTCGTGGACTCTGGGGCACCCCCTGCTCTCAGTGGTCCCAGT
  GGCACCTCCACAAAGCTCACTGTCCAAGTGTGCCTTCGATTCTGCCGCATGAAGGGCTACCAGCTGGCTG
  GTGTGGAGGCTGGTTATGCCTGCTTCTGTGGCTCTGAAAGTGACCTGGCCCGCGGACGTCCAGCCCCTGC
  CACCGACTGTGACCAGATCTGTTTTGGCCACCCAGGCCAGCTCTGTGGAGGCGATGGACGACTAGGCATC
  TATGAAGTGTCTGTGGGCTCCTGCCAGGGAAACTGGTCGGCTCCTCAAGGAGTCATCTACTCCCCGGATT
  TTCCGGATGAGTATGGACCAGACCGGAACTGCAGCTGGGTATTGGGCCAACTGGGCGCTGTGCTAGAACT
  CACCTTCCGCCTCTTCGAGTTGGCTGATTCTCGAGACCGGCTGGAGCTACGCGACGTCTCGTCCGGCAAC
  CTGCTCCGTGCCTTCGACGGCGCCCATCCGCCGCCTCCGGGACCGCTGCGCCTGCGCACTGCTGCGCTGC
  TGCTCACCTTCCGCAGCGACGCAAGAGGCCATGCTCAAGGCTTCGCGCTCACCTACCGCGGGCTGCAGGA
  TACAGTGGAGGGCAGAGCATCTCCAGAGGATTCAACTGAGAGTCTCGCAGGGGACCCCGATGGGGCTAAC
  GCGAGCTGCAGCCCCAAGCCCGGAGCTGCACAGGCTTCGATAGGTGCCCGAGTCTTCTCCACCGTGACGG
  CCTTCTCTGTGCTGCTGCTGTTGCTCCTGTCCCTACTGCGTTTGCTGCGTCGACGGAGCTGTCTGCTGGC
  TCCAGGAAAAGGGTCTCTGGCCATGGGACCTTCCCGGGGCCCCGGGAGAAGCTGGGCTGTGTGGTACCGC
  CGGCCCCGAGGGGTGGCCCTGCCCTGTCCCCCAGGGGACTCTCAGGCTGAGGGTCCTGCTGCGGGCTACC
  GTCCCCTGAGTGCCTCCAGCCAGAGCTCCTTGCGCTCGCTCGTCTCTGCTCTCTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_028416
• Insert Size: 1386 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC120532, AAI20533
• RefSeq Size: 1579 bp
• RefSeq ORF: 1386 bp
• Locus ID: 73016
• UniProt ID: Q8K1S7
• Cytogenetics: 17 A3.3
• Gene Summary: Receptor for Dickkopf proteins. Cooperates with DKK1/2 to inhibit Wnt/beta-catenin signaling by promoting the endocytosis of Wnt receptors LRP5 and LRP6 (PubMed:12050670). Plays a role in limb development; attenuates Wnt signaling in the developing limb to allow normal limb patterning and can also negatively regulate bone formation (PubMed:18505822).[UniProtKB/Swiss-Prot Function]